Method and microfluidic system for processing organic cells and manufacturing method for producing a microfluidic system for processing organic cells

Patent metadata

Field	Value
Publication number	US-10160943-B2
Application number	US-201514691752-A
Country	US
Kind code	B2
Filing date	Apr 21, 2015
Priority date	Apr 25, 2014
Publication date	Dec 25, 2018
Grant date	Dec 25, 2018

Abstract

A method for processing organic cells includes providing a microfluidic system having a chamber comprising a stationary phase in the form of a plurality of microparticles. The method further includes letting a plurality of organic cells in a mobile phase into the chamber across a first opening of the microfluidic system. The method further includes accumulating the organic cells in a tapered section of the chamber that is upstream of a filter of the microfluidic system that is impermeable to the microparticles. The method further includes flushing a lysis agent into the chamber to resuspend the microparticles and the organic cells in the chamber for a disruption of the organic cells.

First claim

What is claimed is:

1. A method for processing organic cells, comprising:
providing a microfluidic system having a chamber including a stationary phase in the form of a plurality of microparticles, the microfluidic system further including a filter that is impermeable to the microparticles, the filter having a first side arranged toward the chamber and an opposing second side;
letting a plurality of organic cells in a mobile phase into the chamber across a first opening of the microfluidic system;
accumulating the organic cells in a section of the chamber that is upstream of the filter; and
flushing a lysis agent through the filter from the second side toward the first side into the chamber to resuspend the microparticles and the organic cells in the chamber for a disruption of the organic cells causing a release of a nucleic acid from the organic cells in the chamber.

2. The method according to claim 1 , wherein flushing the lysis agent into the chamber further includes flushing the lysis agent into the chamber across a second opening of the microfluidic system that is downstream of the filter.

3. The method according to claim 1 , wherein, after flushing the lysis agent into the chamber, the microparticles are set into motion by coupling one of ultrasound and shockwaves into the chamber.

4. The method according to claim 1 , further comprising at least one of (i) filling the microparticles across the first opening into the chamber, and (ii) packing the microparticles in a tapered section of the chamber.

5. The method according to claim 2 , further comprising introducing a binder across the second opening into the chamber to enable a nucleic acid released from the cells on the basis of the disruption to bind to the microparticles.

6. The method according to claim 5 , further comprising packing the microparticles having the nucleic acids bound to the microparticles in a tapered section of the chamber.

7. The method according to claim 5 , further comprising eluting the nucleic acids from the microparticles and transporting the nucleic acid from the chamber through the filter and one of the second opening and a further opening.

8. The method according to claim 5 , further comprising introducing a cleaning agent through the second opening into the chamber to clean the microparticles having the nucleic acids bound to the microparticles.

9. The method according to claim 1 , wherein, after disrupting the organic cells, nucleic acids are purified on a separate membrane.

10. A method for processing organic cells, comprising:
providing a microfluidic system having a chamber including a stationary phase in the form of a plurality of microparticles, the microfluidic system further including a filter that is impermeable to the microparticles, the filter located in a channel, the filter having a first side arranged toward the chamber and an opposing second side; the channel comprising a second opening of the microfluidic system arranged between the chamber and the filter, the second opening in contact with the microparticles;
letting a plurality of organic cells in a mobile phase into the chamber across a first opening of the microfluidic system;
accumulating the organic cells in a section of the microfluidic system that is upstream of the filter; and
flushing a lysis agent in an upstream direction through the channel into the chamber, the channel arranged to allow the lysis agent to flow into the section of the chamber in which the microparticles are situated to resuspend the microparticles and the organic cells in the chamber for a disruption of the organic cells causing a release of nucleic acids from the organic cells in the chamber.

Assignees

• Bosch Gmbh Robert

Inventors

• Dorrer Christian
• Faltin Bernd

Classifications

• B01L2300/0681

  Filter · CPC title

• C12M29/04

  Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title

• C12M47/06

  Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003) · CPC title

• C12N1/06

  Lysis of microorganisms · CPC title

• C12M25/16

  Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00) · CPC title