Polypeptides having immunoactivating activity and methods of producing the same

Patent metadata

Field	Value
Publication number	US-10160789-B2
Application number	US-201214005388-A
Country	US
Kind code	B2
Filing date	Mar 14, 2012
Priority date	Mar 14, 2011
Publication date	Dec 25, 2018
Grant date	Dec 25, 2018

Abstract

Isolated polypeptides are provided that comprise a cholera toxin B subunit variant having one or more modifications to increase the expression of the polypeptide in a plant cell. Nucleic acids sequences, vectors, and plant cells for expressing the cholera toxin B subunit variant polypeptides are also provided. Further provided are methods for producing the cholera toxin B subunit variant polypeptides that include the steps of transforming a plant cell with a nucleic acid encoding the cholera toxin B subunit variant polypeptides; expressing the variant polypeptides; and purifying the polypeptides. Still further provided are methods of isolating the variant polypeptides that include the steps of obtaining a plant cell expressing the cholera toxin B subunit variant polypeptides; extracting the cholera toxin B subunit variant polypeptides from the plant cell; and purifying the cholera toxin B subunit variant polypeptides. Methods of eliciting an immune response are also provided.

First claim

What is claimed is:

1. An isolated polypeptide produced in a plant cell, the isolated polypeptide comprising the sequence of SEQ ID NO: 4.

2. The polypeptide of claim 1 , wherein the polypeptide further comprises a secretory signal peptide selected from the group consisting of a rice alpha-amylase secretory signal peptide, a Nicotiana plumbagenifolia calreticulin secretory signal peptide, an apple pectinase secretory signal peptide, and a barley alpha-amylase secretory signal peptide.

3. The polypeptide of claim 2 , wherein the secretory signal peptide has an amino acid sequence selected from the group consisting of SEQ ID NOS: 18, 20, 22,and 24.

4. The polypeptide of claim 2 , wherein the secretory signal peptide comprises the rice alpha-amylase secretory signal peptide.

5. A pharmaceutical composition comprising the polypeptide of claim 1 and a pharmaceutically-acceptable vehicle, carrier, or excipient.

6. The pharmaceutical composition of claim 5 , wherein the pharmaceutical composition further comprises an adjuvant.

Assignees

• Matoba Nobuyuki
• Hamorsky Krystal
• Univ Louisville Res Found Inc

Inventors

• Matoba Nobuyuki
• Hamorsky Krystal

Classifications

• A61K2039/542

  oral/gastrointestinal · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K2319/02

  containing a signal sequence · CPC title

• C07K14/28 Primary

  from Vibrionaceae (F) · CPC title

• C12N15/8258

  for the production of oral vaccines (antigens) or immunoglobulins · CPC title