Electron microscopy sample support including porous metal foil

Patent metadata

Field	Value
Publication number	US-10157725-B2
Application number	US-201414911723-A
Country	US
Kind code	B2
Filing date	Aug 12, 2014
Priority date	Aug 13, 2013
Publication date	Dec 18, 2018
Grant date	Dec 18, 2018

Abstract

Some embodiments are directed to an electron microscopy sample support including: a support member; and a metal foil including a porous region. The support member is configured to give structural stability to the metal foil, and the porous region of the metal foil is configured to receive an electron microscopy sample. Also disclosed is a method of manufacturing such an electron microscopy sample support, a method of imaging using such an electron microscopy sample support and an apparatus operable to perform such imaging. The disclosed microscopy specimen support reduces particle motion and/or sample charging in electron microscopy, and thus improve information available from electron micrographs. Appropriately designed and constructed supports may lead to an increased resolution per particle and increased accuracy of angular assignments in 3D reconstructions of, for example, biological specimens, enabling the determination of structures of smaller and more difficult proteins than was previously possible using EM techniques.

First claim

The invention claimed is:

1. An electron microscopy sample support, comprising:
a metal support member including a plurality of spaced support elements arranged to form a mesh; and
a metal foil including a porous region;
the support member being configured to give structural stability to the metal foil, and the porous region of the metal foil being configured to receive an electron microscopy sample,
wherein the support member, the plurality of spaced support elements, and the metal foil are all formed from the same metal.

2. The electron microscopy sample support according to claim 1 , wherein the metal foil is arranged to be in ohmic contact with the support member.

3. The electron microscopy sample support according to claim 1 , wherein the metal foil includes a metal having a high conductivity.

4. The electron microscopy sample support according to claim 1 , wherein the metal foil includes a metal having a high secondary electron generation yield.

5. The electron microscopy sample support according to claim 1 , wherein the metal foil includes a metal having a high mechanical stability.

6. The electron microscopy sample support according to claim 1 , wherein the metal foil includes a non-reactive metal.

7. The electron microscopy sample support according to claim 1 , wherein the metal foil includes a metal compatible with a biological electron microscopy sample.

8. The electron microscopy sample support according to claim 1 , wherein the metal foil includes at least one of a: gold, platinum, palladium, rhodium or hafnium metal foil.

9. The electron microscopy sample support according to claim 1 , wherein the porous region of the metal foil includes a layer of metal including a plurality of holes.

10. The electron microscopy sample support according to claim 9 , wherein each of the holes are dimensioned to receive at least one the electron microscopy sample.

11. The electron microscopy sample support according to claim 1 , wherein the metal foil has a thickness selected to be at least the smallest dimension of the electron microscopy sample.

12. The electron microscopy sample support according to claim 1 , wherein the support member includes a plurality of spaced support elements.

13. The electron microscopy sample support according to claim 12 , wherein the plurality of spaced support elements are arranged to form a mesh.

14. The electron microscopy sample support according to claim 11 , wherein the porous region of the metal foil is arranged to extend across a region of the plurality of spaced support elements.

15. The electron microscopy sample support according to claim 1 , wherein the support member includes a metal.

16. The electron microscopy sample support according to claim 15 , wherein the metal includes at least one of: gold, platinum, palladium, rhodium, or hafnium.

17. The electron microscopy sample support according to claim 1 , wherein the support further includes a graphene layer.

18. The electron microscopy sample support according to claim 17 , wherein the graphene layer is configured to extend across pores in the porous region of the metal foil.

19. The electron microscopy sample support according to claim 17 , wherein the graphene layer is configured to be in ohmic contact with the metal foil.

20. The electron microscopy sample support according to claim 17 , wherein the graphene layer is configured to support the electron microscopy sample.

21. The electron microscopy sample support according to claim 1 , wherein pores in the porous region are configured to receive a radiation sensitive material to be examined using electron microscopy.

22. A method of manufacturing an electron microscopy sample support, the method comprising:
providing a metal support member including a plurality of spaced support elements arranged to form a mesh; and a metal foil including a porous region;
configuring the support member to give structural stability to the metal foil;
configuring the porous region of the metal foil to receive an electron microscopy sample; and
forming all of the support member, the plurality of spaced support elements, and the metal foil from the same metal.

23. A method of imaging an electron microscopy sample, comprising:
configuring the electron microscopy sample on the support according to claim 1 ;
arranging the support in an electron beam of an microscope; and
collecting image data for analysis.

24. An imaging apparatus operable to provide an electron microscopy image of a sample, the apparatus comprising:
an electron microscopy sample mounted on the support according to claim 1 ;
a microscope providing an electron beam arranged to be incident on the support; and
a collection device operable to collect image data for analysis.

25. The electron microscopy sample support according to claim 1 , wherein the metal foil has a mechanical strength at a selected thickness which is sufficient to reduce motion of the metal foil during exposure to less than 2×10 −9 m perpendicular to the metal foil.

26. The electron microscopy sample support according to claim 1 , wherein the metal foil has the mechanical strength at the selected thickness which is sufficient to reduce motion of the metal foil during exposure to less than 2×10 −10 m in a plane of the metal foil.

27. The electron microscopy sample support according to claim 1 , wherein the metal foil has a minimum thickness greater than an average size of evaporated metal crystal grains of the foil.

28. The electron microscopy sample support according to claim 1 , wherein the metal foil is gold and has a thickness of at least 5×10 −8 m.

Assignees

• Medical Res Council
• Res & Innovation Uk

Inventors

• Passmore Lori A
• Russo Christopher J

Classifications

• H01J2237/2004

  Biological samples · CPC title

• H01J37/20 Primary

  Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support · CPC title

• H01J2237/2001

  Maintaining constant desired temperature · CPC title

• H01J2237/26

  Electron or ion microscopes · CPC title

• H01J37/06

  Electron sources; Electron guns · CPC title