Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance and/or biomass and/or yield in plants expressing same

Patent metadata

Field	Value
Publication number	US-10155957-B2
Application number	US-201615278086-A
Country	US
Kind code	B2
Filing date	Sep 28, 2016
Priority date	Jul 24, 2007
Publication date	Dec 18, 2018
Grant date	Dec 18, 2018

Abstract

Provided are methods of increasing tolerance of a plant to abiotic stress, and/or increasing biomass, growth rate, vigor and/or yield of a plant. The methods are effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 90% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:201, 207, 212, 202-206, 208-211, 213-391, 1655, 961-1529, and 1660-1663. Also provided are polynucleotides, nucleic acid constructs, polypeptides and transgenic plants expressing same which can be used to increase tolerance of a plant to abiotic stress, and/or increase biomass, growth rate, vigor and/or yield of a plant.

First claim

What is claimed is:

1. A method of increasing abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency of a plant as compared to the abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency of a control plant of the same species which is grown under the same growth conditions, comprising:
(a) over-expressing within the plant a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 271, and
(b) selecting from plants resultant of step (a) a plant exhibiting an increased biomass, growth rate, seed yield, abiotic stress tolerance and/or nitrogen use efficiency as compared to a control plant of the same species which is grown under the same growth conditions, wherein the abiotic stress is selected from the group consisting of salinity stress and nitrogen deficiency,

thereby increasing the abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency of the plant as compared to the abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency of the control plant of the same species which is grown under the same growth conditions.

2. A method of producing a crop comprising growing a crop plant over-expressing a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 271, wherein said crop plant is derived from parent plants selected for increased abiotic stress tolerance, increased seed yield, increased biomass, increased growth rate and/or increased nitrogen use efficiency as compared to the abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency of a control plant of the same species which is grown under the same growth conditions, wherein the abiotic stress is selected from the group consisting of salinity stress and nitrogen deficiency, and said crop plant having said increased abiotic stress tolerance, said increased seed yield, said increased biomass, said increased growth rate and/or said increased nitrogen use efficiency, thereby producing the crop.

3. The method of claim 2 , wherein said growing said crop plant over-expressing said polypeptide is performed under salinity conditions.

4. The method of claim 2 , wherein said growing said crop plant over-expressing said polypeptide is performed under nitrogen deficient conditions.

5. A method of selecting a plant having increased abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency as compared to a control plant of the same species which is grown under the same growth conditions, the method comprising:
(a) providing plants transformed with a nucleic acid construct comprising a polynucleotide comprising a nucleic acid sequence encoding a polypeptide, wherein said polypeptide comprises the amino acid sequence set forth by SEQ ID NO: 271, and a heterologous promoter for directing transcription of said nucleic acid sequence in a plant cell, and;
(b) selecting from said plants a plant having increased abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency as compared to a control plant of the same species which is grown under the same growth conditions, wherein the abiotic stress is selected from the group consisting of salinity stress and nitrogen deficiency,
thereby selecting the plant having increased abiotic stress tolerance, seed yield, biomass, growth rate and/or nitrogen use efficiency as compared to the control plant of the same species which is grown under the same growth conditions.

6. The method of claim 5 , further comprising:
(c) growing a crop of said plant transformed with the nucleic acid construct.

7. The method of claim 5 , wherein said growing comprises seeding seeds and/or planting plantlets of said plant transformed with the nucleic acid construct.

8. The method of claim 5 , wherein said nucleic acid sequence is set forth by SEQ ID NO: 1559 or 81.

9. A method of increasing root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or increased seed yield of a plant as compared to the root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or seed yield of a control plant of the same species which is grown under the same growth conditions, comprising:
(a) over-expressing within the plant a polypeptide comprising the amino acid sequence set forth by SEQ ID NO: 271, and
(b) selecting from plants resultant of step (a) a plant exhibiting an increased root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or seed yield as compared to a control plant of the same species which is grown under the same growth conditions,
thereby increasing the root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or seed yield of the plant as compared to the root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or seed yield of the control plant of the same species which is grown under the same growth conditions.

10. The method of claim 9 , wherein said selecting in step (b) is performed under normal conditions.

11. The method of claim 9 , wherein said selecting in step (b) is performed under an abiotic stress, wherein said abiotic stress is selected from the group consisting of salinity stress and nitrogen deficiency.

12. The method of claim 9 , wherein said selecting said plant exhibiting said increased root length is performed under normal conditions.

13. The method of claim 9 , wherein said selecting said plant exhibiting said increased root coverage is performed under nitrogen deficient conditions.

14. The method of claim 9 , wherein said selecting said plant exhibiting said increased growth rate of rosette area is performed under a salinity stress.

15. The method of claim 9 , wherein said selecting said plant exhibiting said increased growth rate of rosette diameter is performed under a salinity stress.

16. The method of claim 9 , wherein said selecting said plant exhibiting said increased growth rate of rosette area is performed under normal conditions.

17. The method of claim 9 , wherein said selecting said plant exhibiting said increased growth rate of rosette diameter is performed under normal conditions.

18. The method of claim 9 , wherein said selecting said plant exhibiting said increased seed yield is performed under a salinity stress.

19. The method of claim 9 , wherein said selecting said plant exhibiting said increased seed yield is performed under normal conditions.

20. A method of selecting a plant having increased root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or seed yield as compared to a control plant of the same species which is grown under the same growth conditions, the method comprising:
(a) providing plants transformed with a nucleic acid construct comprising a polynucleotide comprising a nucleic acid sequence encoding a polypeptide, wherein said polypeptide comprises the amino acid sequence set forth by SEQ ID NO: 271, and a heterologous promoter for directing transcription of said nucleic acid sequence in a plant cell, and;
(b) selecting from said plants a plant having increased root length, root coverage, growth rate of rosette area, growth rate of rosette diameter, and/or seed yield as compared to a control plant of the same species which is grown under the same growth conditions,
thereby selecting the plant having increased root length, root coverage, gr

Assignees

• Evogene Ltd

Inventors

• Ronen Gil
• Karchi Hagai
• Diber Alex
• Vinocur Basia Judith
• Ayal Sharon
• Emmanuel Eyal
• Gang Michael
• Dimet Dotan

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title