Methods and compositions related to thioesterase enzymes
US-9587231-B2 · Mar 7, 2017 · US
Acetyl-CoA carboxylases
US10155937B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10155937-B2 |
| Application number | US-201615269382-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 19, 2016 |
| Priority date | Mar 15, 2013 |
| Publication date | Dec 18, 2018 |
| Grant date | Dec 18, 2018 |
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Abstract
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The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
First claim
Opening claim text (preview).
What is claimed is: 1. A genetically modified microorganism comprising a heterologous gene encoding an acetyl-CoA carboxylase (ACCase) polypeptide fusion wherein the polypeptide fusion comprises at least two subunits of an ACCase enzyme selected from the group consisting of accA-accB, accA-accC, accA-accD, accB-accC, accB-accD, accC-accD, accA-accB-accC, accA-accB-accD, accA-accC-accD, accB-accC-accD and accA-accB-accC-accD, wherein the genetically modified microorganism further comprises a genetic modification to a nucleotide encoding a functional D-lactate dehydrogenase, pyruvate formate-lyase, phosphate acetyltransferase, pyruvate oxidase, methylglyoxal synthase, acetate kinase, phosphotransacetylase-acetate kinase, or any combination thereof. 2. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for D-lactate dehydrogenase comprises IdhA from E. coli. 3. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for pyruvate formate-lyase comprises pflB from E. coli. 4. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for phosphate acetyltransferase comprises pta from E. coli. 5. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for the pyruvate oxidase comprises poxB from E. coli. 6. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for methylglyoxal synthase comprises mgsA from E. coli. 7. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for acetate kinase comprises ackA from E. coli. 8. The genetically modified microorganism of claim 1 , wherein the nucleotide encoding for phosphotransacetylase-acetate kinase comprises pta-ack from E. coli. 9. A genetically modified microorganism comprising a heterologous gene encoding an acetyl-CoA carboxylase (ACCase) polypeptide fusion wherein the polypeptide fusion comprises at least two subunits of an ACCase enzyme selected from the group consisting of accA-accB, accA-accC, accA-accD, accB-accC, accB-accD, accC-accD, accA-accB-accC, accA-accB-accD, accA-accC-accD, accB-accC-accD and accA-accB-accC-accD, wherein the genetically modified microorganism is capable of producing a C4 to C18 fatty acid chain product. 10. The microorganism of claim 9 , wherein the fatty acid chain product is selected from the group consisting of fatty acid methyl esters and fatty acids.
Assignees
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Classifications
with NAD or NADP as acceptor (1.4.1) · CPC title
Genes encoding for enzymes or proenzymes · CPC title
Saturated compounds having only one carboxyl group and containing hydroxy or O-metal groups · CPC title
- C12N9/93Primary
Ligases (6) · CPC title
Hydroxy-carboxylic acids · CPC title
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Frequently asked questions
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- What does patent US10155937B2 cover?
- The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
- Who is the assignee on this patent?
- Cargill Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/93. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 18 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).