Composition for inducing differentiation

Patent metadata

Field	Value
Publication number	US-10155804-B2
Application number	US-201515307441-A
Country	US
Kind code	B2
Filing date	Apr 30, 2015
Priority date	Apr 30, 2014
Publication date	Dec 18, 2018
Grant date	Dec 18, 2018

Abstract

To provide a differentiation-inducing composition and a method for producing the differentiation-inducing composition, the present disclosure describes use of a degradation product containing at least a portion of the triple helical domain of collagen or atelocollagen.

First claim

The invention claimed is:

1. A differentiation-inducing composition for inducing cell differentiation,
the differentiation-inducing composition comprising:
a degradation product of a collagen or an atelocollagen,
the degradation product containing at least a portion of a triple helical domain of the collagen or the atelocollagen,
the degradation product resulting from:
cleavage of a chemical bond between X 1 and X 2 , between X 2 and G, or between X 4 and G in an amino acid sequence in (1) below within the triple helical domain,
cleavage of a chemical bond between X 1 and X 2 , between X 2 and G, between X 4 and G, between G and X 7 , or between X 14 and G in an amino acid sequence in (2) below within the triple helical domain, or
cleavage of a chemical bond between Y 1 and Y 2 in an amino acid sequence in (3) below at an amino terminus of the triple helical domain,
the amino acid sequence in (1) or (2) being at an amino terminus of the triple helical domain,
a G in each of the amino acid sequences of (1) to (3) which G is at or closest to an amino terminus of the each of the amino acid sequwnces (1) to (3) being a G within a native triple helical domain which G is at or closest to an amino terminus of the native triple helical domain,
(1) -G-X 1 -X 2 -G-X 3 -X 4 -G-X 5 -X 6 -G- (SEQ ID NO: 1),
(2) -G-X 1 -X 2 -G-X 3 -X 4 -G-X 5 -X 6 -G-X 7 -X 8 -G-X 9 -X 10 -G-X 11 -X 12 -G-X 13 -X 14 -G (SEQ ID NO: 14),
(3) -Y 1 -Y 2 -Y 3 -G-Y 4 -Y 5 -G-Y 6 -Y 7 -G-Y 8 -Y 9 -G- (SEQ ID NO: 13),
wherein
G represents glycine,
X 1 and Y 4 each represent proline,
X 2 and Y 5 each represent methionine,
X 3 and Y 6 each represent proline or leucine,
X 4 and Y 7 each represent alanine or serine,
X 5 and Y 8 each represent proline or serine,
X 6 and Y 9 each represent arginine, and
X 7 to X 14 and Y 1 to Y 3 each represent an amino acid.

2. The differentiation-inducing composition according to claim 1 ,
wherein
the cleavage in the amino acid sequence in (1), (2), or (3) is within at least one of an α1chain and an α2 chain of the collagen or the atelocollagen.

Assignees

• Univ Kinki

Inventors

• Morimoto Koichi
• KUNII Saori
• Yamamoto Ei
• Ito Hiroyuki
• Kuboki Yoshinori

Classifications

• A23J3/06

  Gelatine · CPC title

• A61K38/17

  from animals; from humans {(enzyme inhibitors A61K38/005)} · CPC title

• A23J3/34

  using enzymes · CPC title

• C07K14/78 Primary

  Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] · CPC title

• C12P21/06

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title