Microfabricated elastomeric valve and pump systems

Patent metadata

Field	Value
Publication number	US-10155250-B2
Application number	US-201615173262-A
Country	US
Kind code	B2
Filing date	Jun 3, 2016
Priority date	Jun 28, 1999
Publication date	Dec 18, 2018
Grant date	Dec 18, 2018

Abstract

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

First claim

What is claimed is:

1. The method of using a microfluidic device, comprising:
flowing a sample into a looped channel of the microfluidic device;
pumping the sample around the loop channel with a peristaltic action, wherein the peristaltic action comprises sequential activation of a series of individually addressable valves arranged along the looped channel.

2. The method of claim 1 , wherein the series of individually addressable valves comprise three valves.

3. The method of claim 2 , wherein each of the three valves is an intersection of an individually addressable control line over the looped channel.

4. The method of claim 2 , wherein the activation of the three valves corresponds to a successive “101, 100, 110, 010, 011, 001” pattern.

5. The method of claim 1 , wherein the microfluidic device is an elastomeric device.

6. The method of claim 1 , wherein the pumping passes the sample over a DNA array.

7. The method of claim 1 , wherein the pumping mixes a sample.

8. The method of claim 1 , wherein the microfluidic device comprises a sorting apparatus.

9. The method of claim 8 , further comprising cell sorting on the microfluidic device.

10. The method of claim 1 wherein the microfluidic device comprises a cell pen or cell cage.

11. The method of claim 10 , wherein the microfluidic device comprises a cell pen.

12. The method of claim 11 , further comprising storing a cell in the cell pen.

13. The method of claim 10 , wherein the microfluidic device comprises a cell cage.

14. The method of claim 13 , further comprising storing a cell in the cell cage.

15. The method of claim 14 , further comprising providing a continuous intake of food and expulsion of wastes to maintain cell viability.

16. The method of claim 10 , further comprising disrupting cells in the microfluidic device.

Assignees

• California Inst Of Techn

Inventors

• Chou Hou-Pu
• Quake Stephen R

Classifications

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title

• B01J2219/00355

  peristaltic · CPC title

• F16K99/0059

  actuated by a pilot fluid · CPC title

• B01L2400/0655

  pinch valves · CPC title