Assays for measuring nucleic acids
US-2024226890-A1 · Jul 11, 2024 · US
Methods and devices for detection and measurement of analytes
US10145844B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10145844-B2 |
| Application number | US-201615189263-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 22, 2016 |
| Priority date | Jan 31, 2012 |
| Publication date | Dec 4, 2018 |
| Grant date | Dec 4, 2018 |
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Abstract
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Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for optimizing sensitivity and/or selectivity of a sensor for one or more analytes, comprising linking one or more types of aptamers to a substrate with a self-assembled monolayer (SAM) linkage, the SAM linkage having a desired linking spacing and/or length to form a functionalized surface on the substrate, wherein the SAM linkage is formed using dithiobis-N-succinimidyl propionate (DTSP) and (1-mercapto-11-undecyl) tri(ethylene glycol) (PEG3), wherein PEG3 prevents non-specific adsorption of proteins, and wherein a carboxylic moiety on DTSP forms a stable bonding with the aptamer. 2. The method of claim 1 , wherein at least one packing density and/or length of the SAM linkage affects a surface plasmon resonance (SPR) signal. 3. The method of claim 1 , wherein at least one packing density and/or length of the SAM linkage affects electrochemical impedance spectroscopy (EIS) signals.
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Classifications
Haemoglobins; Myoglobins · CPC title
involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title
being a microscope, e.g. atomic force microscopy [AFM] · CPC title
Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith {; Nucleic acids binding to non-nucleic acids, e.g. aptamers} · CPC title
- C12Q1/6825Primary
Nucleic acid detection involving sensors · CPC title
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Frequently asked questions
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- What does patent US10145844B2 cover?
- Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.
- Who is the assignee on this patent?
- Univ Toledo
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6825. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 04 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).