Luminescent Iridium(III) complex and its uses thereof for the G-quadruplex-based switch-on rapid detection of lead ions

Patent metadata

Field	Value
Publication number	US-10145825-B2
Application number	US-201615291041-A
Country	US
Kind code	B2
Filing date	Oct 11, 2016
Priority date	Oct 12, 2015
Publication date	Dec 4, 2018
Grant date	Dec 4, 2018

Abstract

A luminescent Ir(III) complex it used to develop a label-free G-quadruplex-based assay for lead ions in a liquid or aqueous solution. In particular, the present invention describes method for monitoring lead ion concentration in water.

First claim

What we claim is:

1. A system for detecting lead ions in a liquid comprising:
at least one luminescent iridium(III) complex and
at least one G-quadruplex-forming sequence;

wherein said at least one G-quadruplex-forming sequence is a single stranded oliogmer in the absence of lead ions and said at least one G-quadruplex-forming sequence forms a G-quadruplex structure in the presence of lead ions and wherein said at least one G-quadruplex-forming sequence reacts with said at least one luminescent iridium(III) complex to emit luminescent emission and wherein said at least one luminescent iridium(III) complex is Ir(III) complex, [Ir(epyd) 2 (dclphen)]PF 6 , wherein epyd =2-(4-ethylphenyl)pyridine; and dclphen =4,7-dichloro-1,10-phenanthroline.

2. The system according to claim 1 wherein said at least one G-quadruplex-forming sequence is PS2.M, 5′-GTGGGTAGGGCGGGTTGG-3′ (SEQ ID NO. 1).

3. The system according to claim 1 wherein said liquid is water.

4. The system according to claim 1 further comprising at least one spectrometer for measurement of said luminescent emission.

5. A method for detecting lead ions in a liquid comprising:
providing the system of claim 1 ;
introducing said liquid to the at least one G-quadruplex-forming sequence to form a first mixture;
adding the at least one luminescent iridium(III) complex to the first mixture to form a second mixture; and measuring luminescent emission of the second mixture.

6. The method of claim 5 further comprising incubating the first mixture at temperature of 32° C. to 99° C. and cooling the first mixture at −18° C. to 32° C.

7. The method according to claim 5 wherein said at least one G-quadruplex-forming sequence is PS2.M, 5′-GTGGGTAGGGCGGGTTGG′ (SEQ ID NO. 1).

8. The method according to claim 5 wherein said liquid is water.

9. The method according to claim 5 wherein luminescent emission is measured using at least one spectrometer using an excitation wavelength range of between 200-400 nm and a emission spectrum is recorded in a wavelength of 460-770 nm.

Assignees

• Univ Hong Kong Baptist Univ
• Univ Macau

Inventors

• Ma Dik-Lung
• Lin Sheng
• Leung Chung-Hang

Classifications

• G01N31/22 Primary

  using chemical indicators (G01N31/02 takes precedence) · CPC title