Starch-derived clathrate-forming compositions
US-11959114-B2 · Apr 16, 2024 · US
Method and compositions for improved lignocellulosic material hydrolysis
US10144941B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10144941-B2 |
| Application number | US-201514851812-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 11, 2015 |
| Priority date | Dec 22, 2011 |
| Publication date | Dec 4, 2018 |
| Grant date | Dec 4, 2018 |
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- Title
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- Abstract
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- Assignees and inventors
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Abstract
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A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.
First claim
Opening claim text (preview).
We claim: 1. A microbial host cell comprising at least one exogenous nucleic acid molecule encoding a Streptomyces sp ActE (SActE) enzyme, wherein said enzyme is selected from the group consisting of: SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CMB33), SActE_0482 (GH5), SActE_0265 (GH10), SActE_2347 (GH5), SActE_0357 (CE4), SActE_0358 (GH11), SActE_1310 (PL3), SActE_3717 (GH9), SActE_4638, SActE_4738 (GH16), SActE_4755 (GH64), SActE_5457 (GH46), SActE_5647 (GH87), and SActE_5978 (PL1), and wherein the enzyme is expressed in the microbial host cell. 2. The microbial host cell of claim 1 , wherein enzyme: SActE_0237 (GH6) comprises the amino acid sequence SEQ ID NO: 1, SActE_0236 (GH48) comprises the amino acid sequence SEQ ID NO: 2, SActE_3159 (CBM33) comprises the amino acid sequence SEQ ID NO: 3, SActE_0482 (GH5) comprises the amino acid sequence SEQ ID NO: 4, SActE_0265 (GH10) comprises the amino acid sequence SEQ ID NO: 5, SActE_2347 (GH5) comprises the amino acid sequence SEQ ID NO: 6, SActE_0357 (CE4) comprises the amino acid sequence SEQ ID NO: 7, SActE_0358 (GH11) comprises the amino acid sequence SEQ ID NO: 8, SActE_1310 (PL3) comprises the amino acid sequence SEQ ID NO: 9, SActE_3717 (GH9) comprises the amino acid sequence SEQ ID NO: 10, SActE_4638 comprises the amino acid sequence SEQ ID NO: 11, SActE_4738 (GH16) comprises the amino acid sequence SEQ ID NO: 12, SActE_4755 (GH64) comprises the amino acid sequence SEQ ID NO: 13, SActE_5457 (GH46) comprises the amino acid sequence SEQ ID NO: 14, SActE_5647 (GH87) comprises the amino acid sequence SEQ ID NO: 15, and SActE_5978 (PL1) comprises the amino acid sequence SEQ ID NO: 16. 3. The microbial host cell of claim 1 , wherein enzymes SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CBM33), SActE_0482 (GH5), and SActE_3717 (GH9) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 4. The microbial host cell of claim 1 , wherein enzymes SActE_0265 (GH10), SActE_0357 (CE4), SActE_0358 (GH11), and SActE_5978 (PL1) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 5. The microbial host cell of claim 4 , said microbial host cell further comprising an exogenous nucleic acid molecule encoding xylose isomerase SActE_5230. 6. The microbial host cell of claim 1 , wherein enzyme SActE_2347 (GH5) is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 7. The microbial host cell of claim 1 , wherein enzyme SActE_1310 (PL3) is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 8. The microbial host cell of claim 1 , wherein chondroitin/alginate lyase SActE_4638 is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 9. The microbial host cell of claim 1 , wherein enzyme SActE_5647 (GH87) is expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 10. The microbial host cell of claim 1 , wherein enzymes SActE_4738 (GH16) and SActE_4755 (GH64) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 11. The microbial host cell of claim 1 , wherein enzymes SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CBM33), SActE_0482 (GH5), SActE_0265 (GH10), SActE_2347 (GH5), SActE_0358 (GH11), SActE_1310 (PL3), and SActE_3717 (GH9) are expressed in the microbial host cell from the at least one exogenous nucleic acid molecule. 12. The microbial host cell of claim 1 , wherein said microbial host cell is selected from the group consisting of Streptomyces lividans, Trichoderma reesei, Saccharomyces cerevisiae , and Escherichia coli. 13. A Streptomyces sp. ActE strain host cell comprising at least one exogenous nucleic acid molecule encoding a Streptomyces sp ActE (SActE) enzyme, wherein said enzyme is selected from the group consisting of: SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CMB33), SActE_0482 (GH5), SActE_0265 (GH10), SActE_2347 (GH5), SActE_0357 (CE4), SActE_0358 (GH11), SActE_1310 (PL3), SActE_3717 (GH9), SActE_4638, SActE_4738 (GH16), SActE_4755 (GH64), SActE_5457 (GH46), SActE_5647 (GH87), and SActE_5978 (PL1), and wherein the enzyme is expressed in the Streptomyces sp. ActE strain host cell. 14. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzyme: SActE_0237 (GH6) comprises the amino acid sequence SEQ ID NO: 1, SActE_0236 (GH48) comprises the amino acid sequence SEQ ID NO: 2, SActE_3159 (CBM33) comprises the amino acid sequence SEQ ID NO: 3, SActE_0482 (GH5) comprises the amino acid sequence SEQ ID NO: 4, SActE_0265 (GH10) comprises the amino acid sequence SEQ ID NO: 5, SActE_2347 (GH5) comprises the amino acid sequence SEQ ID NO: 6, SActE_0357 (CE4) comprises the amino acid sequence SEQ ID NO: 7, SActE_0358 (GH11) comprises the amino acid sequence SEQ ID NO: 8, SActE_1310 (PL3) comprises the amino acid sequence SEQ ID NO: 9, SActE_3717 (GH9) comprises the amino acid sequence SEQ ID NO: 10, SActE_4638 comprises the amino acid sequence SEQ ID NO: 11, SActE_4738 (GH16) comprises the amino acid sequence SEQ ID NO: 12, SActE_4755 (GH64) comprises the amino acid sequence SEQ ID NO: 13, SActE_5457 (GH46) comprises the amino acid sequence SEQ ID NO: 14, SActE_5647 (GH87) comprises the amino acid sequence SEQ ID NO: 15, and SActE_5978 (PL1) comprises the amino acid sequence SEQ ID NO: 16. 15. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzymes SActE_0237 (GH6), SActE_0236 (GH48), SActE_3159 (CBM33), SActE_0482 (GH5), and SActE_3717 (GH9) are expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 16. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzymes SActE_0265 (GH10), SActE_0357 (CE4), SActE_0358 (GH11), and SAclE_5978 (PL1) are expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 17. The Streptomyces sp. ActE strain host cell of claim 16 , said Streptomyces sp. ActE strain host cell further comprising an exogenous nucleic acid molecule encoding xylose isomerase SActE_5230. 18. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzyme SActE_2347 (GH5) is expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 19. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzyme SActE_1310 (PL3) is expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 20. The Streptomyces sp. ActE strain host cell of claim 13 , wherein chondroitin/alginate lyase SActE_4638 is expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 21. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzyme SActE_5647 (GH87) is expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 22. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzymes SActE_4738 (GH16) and SActE_4755 (GH64) are expressed in the Streptomyces sp. ActE strain host cell from the at least one exogenous nucleic acid molecule. 23. The Streptomyces sp. ActE strain host cell of claim 13 , wherein enzymes SAct
Assignees
Inventors
Classifications
Beta-mannosidase (3.2.1.25), i.e. mannanase · CPC title
acting on beta-1,4-glucosidic bonds · CPC title
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
Beta-mannosidase (3.2.1.25), i.e. mannanase · CPC title
Cellulose 1,4-beta-cellobiosidase (3.2.1.91) · CPC title
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Frequently asked questions
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- What does patent US10144941B2 cover?
- A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.
- Who is the assignee on this patent?
- Wisconsin Alumni Res Found
- What technology area does this patent fall under?
- Primary CPC classification C12P19/14. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 04 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).