Preparation method of yeast cell immobilization medium and application thereof

Patent metadata

Field	Value
Publication number	US-10144925-B2
Application number	US-201214651071-A
Country	US
Kind code	B2
Filing date	Dec 13, 2012
Priority date	Dec 13, 2012
Publication date	Dec 4, 2018
Grant date	Dec 4, 2018

Abstract

Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium.

First claim

The invention claimed is:

1. A preparation method of a yeast cell immobilization medium, which comprises the following steps:
(1) boiling a fiber material in a medium consisting of boiling water followed by drying;
(2) soaking the fiber material in a surface modifier aqueous solution at a concentration of 1-100 g/L, adjusting a pH of the solution to 7.0 with hydrochloric acid, and then fully rinsing the fiber material in deionized water followed by drying;
(3) soaking the fiber material in a cross-linking agent aqueous solution at a concentration of 1-100 g/L, and then fully rinsing the fiber material in deionized water followed by drying; and
(4) attaching the fiber material to a supporting framework,
wherein, said surface modifier is succinimide, and said cross-linking agent is 2-methyl-1,3-malondialdehyde.

2. The preparation method according to claim 1 , wherein said fiber material is one or more selected from the group consisting of cotton fabric; activated carbon fabric; polyester fiber; silk; bamboo fiber; polyvinyl alcohol fiber; non-woven fabrics; polyurethane foam; bagasse and cornstalk.

3. The preparation method according to claim 2 , wherein said polyvinyl alcohol fiber is vinylon.

4. The preparation method according to claim 2 , wherein said non-woven fabrics is selected from a group consisting of dacron, polypropylene fiber, nylon, spandex and acrylic.

5. The preparation method according to claim 2 , wherein said activated carbon fabric is activated carbon fabric cotton.

6. The preparation method according to claim 1 , wherein a concentration of said hydrochloric acid is 0.5-2 mol/L.

7. The preparation method according to claim 1 , wherein said supporting framework is steel wire gauze.

8. The preparation method according to claim 1 , wherein a concentration of succinimide is 10 g/L, and a concentration of 2-methyl-1,-3 -malondialdehyde is 10 g/L.

9. A preparation method of a yeast cell immobilization medium, which comprises the following steps:
(1) boiling a fiber material in a medium consisting of boiling water for 0.1-10 hours followed by drying at 20-100° C., and storing it at 4° for use;
(2) soaking the fiber material in a surface modifier aqueous solution at a concentration of 1-100 g/L for 1-50 hours, adjusting a pH of the solution to 7.0 with hydrochloric acid, fully rinsing the fiber material in deionized water followed by drying at 60° C., and storing it at 4° C. for use;
(3) soaking the fiber material in a cross-linking agent aqueous solution at a concentration of 1-100 g/L for 1-50 hours, fully rinsing the fiber material in deionized water followed by drying at 60° C., and storing it at 4° C. for use; and
(4) tilinq the fiber material on a supporting framework to make it attached to the supporting framework, and then rolling the framework into a cylinder,
wherein, said surface modifier is succinimide, and said cross-linking agent is 2-methyl-1,3-malondialdehyde.

10. The preparation method according to claim 9 , wherein said fiber material is one or more selected from the group consisting of cotton fabric; activated carbon fabric; polyester fiber; silk; bamboo fiber; polyvinyl alcohol fiber; non-woven fabrics; polyurethane foam; bagasse and cornstalk.

11. The preparation method according to claim 10 , wherein said polyvinyl alcohol fiber is vinylon.

12. The preparation method according to claim 10 , wherein said non-woven fabrics is selected from the group consisting of dacron, polypropylene fiber, nylon, spandex and acrylic.

13. The preparation method according to claim 10 , wherein said activated carbon fabric is activated carbon fabric cotton.

14. The preparation method according to claim 9 , wherein a concentration of said hydrochloric acid is 0.5-2 mol/L.

15. The preparation method according to claim 9 , wherein said supporting framework is steel wire gauze.

16. The preparation method according to claim 9 , wherein a concentration of succinimide is 10 g/L, and a concentration of 2-methyl-1,3-malondialdehyde is 10 g/L.

17. The preparation method according to claim 9 , wherein boiling is 1 hour in step (1), soaking is 2 hours in step (2), and soaking is 2 hours in step (3).

Assignees

• Nanjing University Of Technology

Inventors

• Ying Hanjie
• Chen Yong
• Liu Qingguo
• Wu Jinglan
• Chen Xiaochun
• Xie Jingjing

Classifications

• C12N11/12 Primary

  Cellulose or derivatives thereof · CPC title

• D06M13/418

  Cyclic amides, e.g. lactams; Amides of oxalic acid · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12N11/08 Primary

  the carrier being a synthetic polymer · CPC title

• D06M13/123

  Polyaldehydes; Polyketones · CPC title