Imaging flow cytometer using spatial-temporal transformation
US-2024094129-A1 · Mar 21, 2024 · US
US10142566B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10142566-B2 |
| Application number | US-201615145031-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 3, 2016 |
| Priority date | Feb 1, 2013 |
| Publication date | Nov 27, 2018 |
| Grant date | Nov 27, 2018 |
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An image acquisition system 1 includes: a light source 3 which outputs illumination light; an optical scanner 7 which scans a sample S with the illumination light; an optical scanner control unit 9 ; a detection optical system 15, 17 which focuses fluorescence from the sample S; an imaging device 19 which has a light receiving surface 19 c in which a plurality of pixel rows 19 d are arranged, and an imaging control section 19 b , and which can perform signal readout of each of the plurality of pixel rows 19 d from the light receiving surface 19 c ; and a calculation unit 21 which calculates an interval of signal readout between adjacent pixel rows 19 d , based on a moving speed of an illuminated region on the light receiving surface 19 c ; the imaging control section 19 b controls signal readout of each pixel row 19 d , based on the interval of the signal readout thus calculated.
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The invention claimed is: 1. An imaging method for using a rolling readout type camera including a light receiving section in which a plurality of pixel rows are arranged, comprising: setting a first parameter for setting a time interval of start timing of signal readout period of each of adjacent pixel rows; setting a first count of a driving clock defining a time interval of start timing of signal readout period of each of adjacent pixel rows based on the set first parameter; and controlling signal readout of each of pixel rows based on the set first count. 2. The imaging method according to claim 1 , wherein controlling generates a readout start signal of each of the pixel rows based on the set first count. 3. The imaging method according to claim 1 , further comprising: generating a readout start signal and a reset signal to control an exposure period in each of the pixel rows. 4. The imaging method according to claim 3 , wherein a length of the exposure period is a time set so as to control a number of simultaneously exposed pixel rows is a determined number of pixel rows. 5. The imaging method according to claim 3 , wherein a length of the exposure period is a time set based on a determined number of pixel rows. 6. The imaging method according to claim 1 , further comprising: scanning an object with an illumination light by an optical scanner; capturing an image of the object by the rolling readout type camera. 7. The imaging method according to claim 6 , further comprising: synchronizing the rolling readout type camera and the optical scanner. 8. The imaging method according to claim 6 , wherein the first parameter is set so as to synchronize the rolling readout type camera and the optical scanner. 9. A rolling readout type camera, the camera comprising: a light receiving section in which a plurality of pixel rows are arranged; and an imaging control section configured to control signal readout of each of pixel rows of the light receiving section and to perform signal readout by rolling readout of each pixel rows; and an external signal receiving section coupled to the imaging control section and for receiving an external signal, wherein the external signal receiving section is coupled to a calculation unit which generates the external signal including a first data calculated by the calculation unit, wherein the imaging control section controls the signal readout based on a first count of a driving clock defining a time interval of start timing of signal readout period of each of adjacent pixel rows, and wherein the first count of the driving clock is a count set based on the first data for setting a time interval of start timing of the signal readout period of each of adjacent pixel rows. 10. The rolling readout type camera according to claim 9 , wherein the imaging control section generates a readout start signal of each of the pixel rows based on the set first count. 11. The rolling readout type camera according to claim 9 , wherein the imaging control section controls an exposure period in each of the pixel rows by generating a readout start signal and a reset signal. 12. The rolling readout type camera according to claim 11 , wherein a length of the exposure period is a time set so as to control a number of simultaneously exposed pixel rows is a determined number of pixel rows. 13. The rolling readout type camera according to claim 11 , wherein a length of the exposure period is a time set based on a determined number of pixel rows. 14. An image acquisition system for scanning an object with illumination light to acquire an image of the object, comprising: an optical scanner configured to scan the object with an illumination light; the rolling readout type camera of claim 9 configured to capture the image of the object. 15. The image acquisition system according to claim 14 , wherein the optical scanner and the rolling readout type camera are synchronized. 16. The image acquisition system according to claim 14 , wherein the parameter is set so as to synchronize the rolling readout type camera and the optical scanner. 17. The image acquisition system according to claim 14 , wherein image acquisition system is a light sheet microscopy system. 18. An imaging method for using a rolling readout type camera including a light receiving section in which a plurality of pixel rows are arranged, comprising: setting a parameter for setting a time interval of start timing of signal readout period of each of adjacent pixel rows; setting a time interval of start timing of signal readout period of each of adjacent pixel rows based on the set parameter; generating a clock, wherein the clock has a frequency corresponding to the set time interval. 19. An imaging method for using a rolling readout type camera including a light receiving section in which a plurality of pixel rows are arranged, comprising: setting a first parameter for setting a time interval of start timing of signal readout period of each of adjacent pixel rows; setting a first count of a driving clock based on the set first parameter; setting a time of a delay defining the time interval of start timing of signal readout period of each of adjacent pixel rows based on the set first parameter; and controlling signal readout of each of pixel rows based on the set first count and the set time of the delay period. 20. The imaging method according to claim 19 , wherein controlling generates a readout start signal of each of the pixel rows based on the set first count and the set delay period. 21. The imaging method according to claim 19 , further comprising: generating a readout start signal and a reset signal to control an exposure period in each of the pixel rows. 22. The imaging method according to claim 21 , wherein a length of the exposure period is a time set so as to control a number of simultaneously exposed pixel rows is a determined number of pixel rows. 23. The imaging method according to claim 21 , wherein a length of the exposure period is a time set based on a determined number of pixel rows. 24. The imaging method according to claim 19 , further comprising: scanning an object with an illumination light by an optical scanner; capturing an image of the object by the rolling readout type camera. 25. The imaging method according to claim 24 , further comprising: synchronizing the rolling readout type camera and the optical scanner. 26. The imaging method according to claim 24 , wherein the first parameter is set so as to synchronize the rolling readout type camera and the optical scanner. 27. A rolling readout type camera, the camera comprising: a light receiving section in which a plurality of pixel rows are arranged; and an imaging control section configured to control signal readout of each of pixel rows of the light receiving section and to perform signal readout by rolling readout of each pixel rows, wherein the imaging control section controls the signal readout based on a first count of a driving clock and a time of a delay defining a time interval of start timing of signal readout period of each of adjacent pixel rows, and wherein the time of a delay is a value set based on a determined first parameter for setting a time interval of start timing of the signal readout period of each of adjacent pixel rows. 28. The rol
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