Modified live vaccine of Mycoplasma bovis, methods of producing modified live Mycoplasma bovis vaccines, combination vaccines and methods of treatment
US-9339533-B2 · May 17, 2016 · US
Method for detecting Streptococcus bacterium contained in milk
US10139409B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10139409-B2 |
| Application number | US-201415105481-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 17, 2014 |
| Priority date | Dec 18, 2013 |
| Publication date | Nov 27, 2018 |
| Grant date | Nov 27, 2018 |
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- Title
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Abstract
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The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of various Streptococcus bacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is a Streptococcus bacterium or not by using the milk, as well as a detection method using an immunochromatographic device. There is provided a method for lysing a Streptococcus bacterium, which comprises the step of mixing a lysis agent containing a lytic enzyme with milk obtained form a livestock animal to lyse a Streptococcus bacterium existing in the milk. The lytic enzyme is preferably at least one selected from the group consisting of lysozyme, labiase, and β-N-acetylglucosaminidase.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for lysing a Streptococcus bacterium contained in milk, which comprises the step of mixing a lysis agent containing lysozyme, labiase, and a nonionic surfactant with the milk to lyse the bacterium existing in the milk, wherein, in the step of mixing the lysis agent with the milk to lyse the bacterium existing in the milk, final concentration of the nonionic surfactant is not higher than 3%. 2. The lysis method according to claim 1 , wherein the bacterium is Streptococcus uberis or Streptococcus agalactiae. 3. The lysis method according to claim 1 , wherein the nonionic surfactant comprises a polyoxyethylene alkyl phenyl ether and/or a polyoxyethylene sorbitan fatty acid ester. 4. The lysis method according to claim 1 , wherein, in the step of mixing the lysis agent with the milk to lyse the bacterium existing in the milk, final concentration of the nonionic surfactant is not lower than 0.03% and not higher than 3%. 5. The lysis method according to claim 4 , wherein, in the step of mixing the lysis agent with the milk to lyse the bacterium existing in the milk, final concentration of lysozyme is not lower than 0.5 mg/ml and not higher than 200 mg/ml; and/or in the step of mixing the lysis agent with the milk to lyse the bacterium existing in the milk, final concentration of labiase is not lower than 0.05 mg/ml and not higher than 20 mg/ml.
Assignees
Inventors
Classifications
hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title
of the antigen-antibody type, e.g. protein A, G or L chromatography · CPC title
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96) · CPC title
relating to the preparation of the feed · CPC title
Lysozyme (3.2.1.17) · CPC title
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Frequently asked questions
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- What does patent US10139409B2 cover?
- The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of various Streptococcus bacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is a Streptococcus bacterium or not by using the milk, as well as a detection method using an immunochromatographi…
- Who is the assignee on this patent?
- Asahi Chemical Ind
- What technology area does this patent fall under?
- Primary CPC classification G01N33/56944. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Nov 27 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).