Sample heating method and heating control device

Patent metadata

Field	Value
Publication number	US-10139134-B2
Application number	US-201113996949-A
Country	US
Kind code	B2
Filing date	Dec 19, 2011
Priority date	Dec 21, 2010
Publication date	Nov 27, 2018
Grant date	Nov 27, 2018

Abstract

A microchip includes a vessel portion, an elastic member, and a flow channel which leads a liquid sample to the vessel portion. After a liquid sample is put in the vessel portion, the liquid sample is heated while pressure is applied with respect to an inner portion of the vessel portion.

First claim

What is claimed is:

1. A sample heating method which uses a microchip, comprising the steps of:
wherein the microchip includes: a vessel portion in which at least a portion is configured of an elastic member; and a flow channel which leads a liquid sample to the vessel portion,
heating the liquid sample while applying pressure to the liquid sample from outside of the vessel portion,
wherein the microchip further includes an expansion limiting member which is provided around the vessel portion and limits expansion of the elastic member,
wherein at least a portion of the expansion limiting member is configured of either a heat generation member or a heat transfer member, and
wherein the liquid sample is heated in a state where pressure is applied with respect to the inner portion of the vessel portion and thus, the vessel portion contacts either the heat generation member or the heat transfer member of the expansion limiting member.

2. The sample heating method according to claim 1 ,
wherein the liquid sample is heated while pressure is applied with respect to the inner portion of the vessel portion through the flow channel.

3. The sample heating method according to claim 1 ,
wherein the expansion limiting member is configured of either the heat generation member or the heat transfer member, and the liquid sample is heated in a state where pressure is applied with respect to the inner portion of the vessel portion through the flow channel, and thus, the entire vessel portion contacts the expansion limiting member.

4. The sample heating method according to claim 1 ,
wherein a concave portion is provided in the expansion limiting member, and the liquid sample is heated in a state where at least a portion of the expanded vessel portion contacts the concave portion.

5. The sample heating method according to claim 1 ,
wherein the expansion limiting member includes an upper substrate provided over an upper surface side of the vessel portion and a lower substrate provided over a lower surface side of the vessel portion,
wherein at least one of the upper substrate and the lower substrate is configured of either the heat generation member or the heat transfer member, and the liquid sample is heated in a state where the vessel portion contacts the upper substrate or the lower substrate which configures either the heat generation member or the heat transfer member.

6. The sample heating method according to claim 5 ,
wherein both of the upper substrate and the lower substrate are configured of either the heat generation member or the heat transfer member, and the liquid sample is heated in a state where the upper surface side of the vessel portion closely contacts the upper substrate and the lower surface side of the vessel portion closely contacts the lower substrate.

7. The sample heating method according to claim 6 ,
wherein the upper substrate is configured of a first heat transfer member, the lower substrate is configured of a second heat transfer member having higher thermal conductivity than that of the first heat transfer member, and
wherein the lower substrate is controlled so as to heat the liquid sample at a first temperature and a second temperature lower than the first temperature, the upper member is controlled so as to heat the liquid sample at a constant temperature, and the liquid sample is alternately heated at the first temperature and the second temperature.

8. The sample heating method according to claim 5 ,
wherein a concave portion is provided over at least one of the upper substrate and the lower substrate, and at least a portion of the expanded vessel portion contacts the concave portion and thus, the liquid sample is heated.

9. The sample heating method according to claim 1 ,
wherein the microchip includes:
a first film-like elastic member; and
a second film-like elastic member which is stacked so as to contact the first elastic member,
wherein the first elastic member and the second elastic member contact each other around the vessel portion and the flow channel, and thus, the flow channel and the vessel portion are formed respectively in a gap between the first elastic member and the second elastic member.

10. The sample heating method according to claim 1 ,
wherein the sample includes at least DNA, and an amplification reaction (Polymerase Chain Reaction (PCR)) of DNA is performed by heating the sample.

Assignees

• Asogawa Minoru
• Hagiwara Hisashi
• Nec Corp

Inventors

• Asogawa Minoru
• Hagiwara Hisashi

Classifications

• G01N2035/00356

  Holding samples at elevated temperature (incubation) · CPC title

• B01L2300/1822

  using Peltier elements · CPC title

• B01L2200/142

  Preventing evaporation · CPC title

• B01L2300/18

  Means for temperature control · CPC title

• B01L2200/0684

  Venting, avoiding backpressure, avoid gas bubbles · CPC title