Method and device for amplifying and detecting gene using graphene heater

Patent metadata

Field	Value
Publication number	US-10138513-B2
Application number	US-201414554863-A
Country	US
Kind code	B2
Filing date	Nov 26, 2014
Priority date	Aug 29, 2014
Publication date	Nov 27, 2018
Grant date	Nov 27, 2018

Abstract

Provided is a gene amplifying and detecting device. The gene amplifying and detecting device includes: a gene amplifying chip including a chamber formed therein; a reaction solution filled in the chamber and including a fluorescent material; a light source located at one side of the gene amplifying chip; a light detector located at the other side of the gene amplifying chip; and a graphene heater formed on an inner surface or outer surface of the gene amplifying chip so as to heat the reaction solution.

First claim

The invention claimed is:

1. A gene amplifying and detecting device, comprising:
a gene amplifying chip including a chamber formed therein, the gene amplifying chip having a first sidewall and a second sidewall opposite to the first sidewall;
a reaction solution in the chamber and including a fluorescent material;
a light source adjacent to the first sidewall of the gene amplifying chip;
a light detector located adjacent to the second sidewall of the gene amplifying chip; and
a first graphene heater adjacent to the first sidewall of the gene amplifying chip so as to heat the reaction solution to cause a circulating flow of the reaction solution by convection,
wherein the first graphene heater is positioned between the light source and the chamber to transmit light generated from the light source into the chamber.

2. The device of claim 1 , wherein the first graphene heater is in contact with the first sidewall of the gene amplifying chip.

3. The device of claim 1 , wherein the first graphene heater is formed in the gene amplifying chip to be in contact with the chamber.

4. The device of claim 1 , wherein the first graphene heater is formed of a single layer graphene or a multilayer graphene having a transmittance of 80% or more.

5. The device of claim 1 , further comprising a second graphene heater adjacent to the second sidewall of the gene amplifying chip.

6. The gene amplifying and detecting device of claim 5 , wherein:
the second graphene heater is configured to heat at a different temperature from a temperature at which the first graphene heater is configured to heat.

7. The gene amplifying and detecting device of claim 5 , wherein:
the first graphene heater and the second graphene heater are configured to implement a plurality of heating sections respectively for denaturation and annealing.

8. The gene amplifying and detecting device of claim 5 , wherein the second graphene heater is in contact with the chamber or the second sidewall of the gene amplifying chip.

9. The device of claim 5 , wherein the second graphene heater is positioned between the chamber and the light detector, the transmitted light is adsorbed into the fluorescent material included in the reaction solution, the adsorbed light excites and is emitted from the fluorescent material, and the emitted light is detected by the light detector through the second graphene heater.

10. The device of claim 1 , wherein the fluorescent material emits light in proportion to a number of genes included in the reaction solution, and an amount of amplified gene products is measured through an intensity of emitted light measured by the light detector.

11. The device of claim 1 , wherein the gene amplifying chip is formed of a transparent material.

12. The device of claim 1 , wherein the reaction solution includes one of a first reaction solution for gene amplification and a second reaction solution for gene preprocessing for purification and enrichment of genes in a sample.

13. The device of claim 1 , further comprising a first light filter located between the light source and the gene amplifying chip and a second light filter located between the light detector and the gene amplifying chip.

14. The device of claim 1 , further comprising a temperature sensor formed in the gene amplifying chip and configured to sense a temperature of the reaction solution.

15. The device of claim 1 , further comprising a protection layer formed on the graphene heater and including a chemical doping material, a metal material, a glass material, graphene oxide, or a nanostructure.

16. The gene amplifying and detecting device of claim 1 , wherein:
the first graphene heater has a first width and the chamber has a second width parallel to the first width, and the first width is greater than the second width.

17. The gene amplifying and detecting device of claim 1 , wherein the chamber is in a ring shape.

Assignees

• Electronics & Telecommunications Res Inst

Inventors

• Chung Kwang Hyo
• Kim Jin Tae
• Choi Yo Han
• Choi Choon Gi
• Choi Hong Kyw
• Yu Young Jun
• Youn Doo Hyeb
• Choi Jin Sik

Classifications

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• H05B2203/013

  Heaters using resistive films or coatings · CPC title

• B01L2300/1827

  using resistive heater · CPC title

• H05B3/42

  non-flexible · CPC title

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title