Culture container

Patent metadata

Field	Value
Publication number	US-10138454-B2
Application number	US-201515122906-A
Country	US
Kind code	B2
Filing date	Jun 10, 2015
Priority date	Jun 24, 2014
Publication date	Nov 27, 2018
Grant date	Nov 27, 2018

Abstract

A cell-culture container includes a container main body and a presser member. The container main body has a recessed part for containing a cell-culture solution. The presser member is a member that is removably fitted onto an upper side of the recessed part. The presser member has a lower surface, which makes contact with an upper surface of the cell-culture solution in a fitted state, and a bubble-discharge part, which enables the discharge of bubbles in the cell-culture solution to the exterior. Such an arrangement enables accurate observation by the elimination of a meniscus in a culture container.

First claim

The invention claimed is:

1. A culture container comprising:
a transparent container main body having a recessed part configured to contain a culture solution, the recessed part including a bottom part and a tubular part;
a transparent presser member that is removably fitted onto an upper side of the recessed part, the presser member having
a lower-surface part that makes contact with an upper surface of the culture solution in a fitted state, in which the presser member is fitted to the container main body, to press the upper surface of the culture solution, and
a tubular part having a lower end connected an outer circumferential edge of the lower-surface part and an outer circumferential surface proximately opposing an inner circumferential surface of the tubular part of the recessed part, the tubular part opening upward to directly and entirely expose an upper surface of the lower-surface part outside of the culture container through an opening of the tubular part such that the culture solution is observable from the outside of the culture container through the lower-surface part in the fitted state; and

a bubble-discharge passage that is formed by the outer circumferential surface of the tubular part of the presser member and the inner circumferential surface of the tubular part of the recessed part, the outer circumferential surface of the tubular part of the presser member facing the inner circumferential surface of the tubular part of the recessed part, the bubble-discharge passage enabling discharge of bubbles in the culture solution to an exterior.

2. The culture container according to claim 1 , wherein
the lower-surface part of the presser member has a planar end surface that extends horizontally.

3. The culture container according to claim 2 , wherein
the lower-surface part of the presser member is subject, at least partially, to a hydrophilization treatment.

4. The culture container according to claim 3 , wherein
the presser member further includes a liquid-supply passage, and
the liquid-supply passage is used to inject a liquid into the culture solution so as to move bubbles in the culture solution toward the bubble-discharge passage.

5. The culture container according to claim 2 , wherein
the presser member further includes a liquid-supply passage, and
the liquid-supply passage is used to inject a liquid into the culture solution so as to move bubbles in the culture solution toward the bubble-discharge passage.

6. The culture container according to claim 1 , wherein
the lower-surface part of the presser member is subject, at least partially, to a hydrophilization treatment.

7. The culture container according to claim 6 , wherein
the presser member further includes a liquid-supply passage, and
the liquid-supply passage is used to inject a liquid into the culture solution so as to move bubbles in the culture solution toward the bubble-discharge passage.

8. The culture container according to claim 1 , wherein
the presser member further includes a liquid-supply passage, and
the liquid-supply passage is used to inject a liquid into the culture solution so as to move bubbles in the culture solution toward the bubble-discharge passage.

Assignees

• Nissha Printing

Inventors

• Obi Naoko
• Onishi Atsushi
• Iga Toshihiro

Classifications

• C12M29/20 Primary

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• B01L2300/0854

  Double walls · CPC title

• B01L2300/0809

  rectangular shaped · CPC title