Modified bovine G-CSF polypeptides and their uses

Patent metadata

Field	Value
Publication number	US-10138283-B2
Application number	US-50723709-A
Country	US
Kind code	B2
Filing date	Jul 22, 2009
Priority date	Jul 23, 2008
Publication date	Nov 27, 2018
Grant date	Nov 27, 2018

Abstract

Modified bovine G-CSF polypeptides and uses thereof are provided.

First claim

What is claimed is:

1. A bG-CSF polypeptide comprising the sequence of SEQ ID NO: 2, wherein position 134 of SEQ ID NO: 2 is substituted with a non-naturally encoded amino acid,
wherein the non-naturally encoded amino acid is para-acetylphenylalanine,
wherein the non-naturally encoded amino acid is linked to a water soluble polymer comprising a poly(ethylene) glycol moiety,
wherein said water soluble polymer has a molecular weight of about 20 kDa, and
wherein said bG-CSF polypeptide is characterized by inducing a maximal absolute neutrophil count (ANC) about 10-fold greater than pre-treatment ANC, as measured about 72 hours after a single sub-cutaneous injection at 40 μg/kg in a healthy bovine steer calf.

2. A pharmaceutical formulation comprising the bGCSF polypeptide of claim 1 and a pharmaceutically acceptable carrier.

3. A bG-CSF polypeptide comprising the sequence of SEQ ID NO: 2, wherein position 134 of SEQ ID NO: 2 is substituted with a non-naturally encoded amino acid,
wherein the non-naturally encoded amino acid is para-acetylphenylalanine,
wherein the non-naturally encoded amino acid is linked to a water soluble polymer comprising a poly(ethylene) glycol moiety,
wherein said water soluble polymer has a molecular weight of about 20 kDa, and
wherein said bG-CSF polypeptide is characterized by inducing an absolute neutrophil count (ANC) about 7-fold to about 12-fold greater than pre-treatment ANC from about 12 hours to about 96 hours after a single sub-cutaneous injection at 40 μg/kg in a healthy bovine steer calf.

4. A pharmaceutical formulation comprising the bGCSF polypeptide of claim 3 and a pharmaceutically acceptable carrier.

Assignees

• Hays Putnam Anna Maria A
• Knudsen Nick
• Norman Thea
• Koder Alan
• Kraynov Vadim
• Ho Lillian
• Canning Peter C
• Ambrx Inc
• Elanco Us Inc

Inventors

• Hays Putnam Anna-Maria A
• Knudsen Nick
• Norman Thea
• Koder Alan
• Kraynov Vadim
• Ho Lillian
• Canning Peter C

Classifications

• A61P37/04

  Immunostimulants · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• A61P31/00

  Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61P15/14

  for lactation disorders, e.g. galactorrhoea · CPC title