Microparticle sorting device and method of optimizing fluid stream therein

Patent metadata

Field	Value
Publication number	US-10132735-B2
Application number	US-201514737370-A
Country	US
Kind code	B2
Filing date	Jun 11, 2015
Priority date	Mar 30, 2012
Publication date	Nov 20, 2018
Grant date	Nov 20, 2018

Abstract

There is provided a microparticle sorting device that automatically optimizes a fluid stream.
There is provided a microparticle sorting device that includes a voltage supply unit that supplies a driving voltage to a vibratory element that applies vibration to an orifice that produces a fluid stream, a charge unit that imparts charge to at least some droplets ejected from the orifice, deflecting plates, arranged opposing each other with the fluid stream S therebetween, that vary a travel direction of the droplets, and a first image sensor that acquires an image of the droplets passing between the deflecting plates. The microparticle sorting device is equipped with a controller that detects the droplets in the image, sets a standard band corresponding to a width of the droplets before imparting the charge, and controls the driving voltage of the voltage supply unit so as to further decrease a quantity of the droplets detected in areas within a designated number of pixels from the standard band from among the droplets after imparting the charge.

First claim

The invention claimed is:

1. A microparticle sorting device comprising:
a voltage supply unit that supplies a driving voltage to a vibratory element that applies vibration to an orifice that produces a fluid stream;
an image sensor that acquires an image of droplets ejected from the orifice at a position where fluid exiting the orifice breaks into droplets; and
a controller configured to control the driving voltage based on a length of a satellite droplet positioned between the fluid and a microparticle-containing droplet immediately after dividing off from the fluid, and a distance between the microparticle-containing droplet and the fluid.

2. The microparticle sorting device according to claim 1 , wherein the controller controls the driving voltage such that the length along the travel direction becomes a designated length in the image.

3. The microparticle sorting device according to claim 2 , wherein the designated length is 30% to 70% of the distance between the microparticle-containing droplet and the fluid.

4. The microparticle sorting device according to claim 1 , wherein the orifice is formed on a replaceable microchip.

5. A method of optimizing a fluid stream in a microparticle sorting device, comprising:
acquiring an image of droplets ejected from an orifice to which vibration is applied by a vibratory element at a position where fluid exiting the orifice breaks into droplets; and
controlling a driving voltage of the vibratory element based on a length of a satellite droplet positioned between the fluid and a microparticle-containing droplet immediately after dividing off from the fluid, and a distance between the microparticle-containing droplet and the fluid.

6. The method of optimizing a fluid stream according to claim 5 , wherein controlling the driving voltage includes controlling the driving voltage such that the length along the travel direction becomes a designated length in the image.

7. The method of optimizing a fluid stream according to claim 6 , wherein the designated length is 30% to 70% of the distance between the microparticle-containing droplet and the fluid.

8. The method of optimizing a fluid stream according to claim 5 , wherein the orifice is formed on a replaceable microchip.

9. The method of optimizing a fluid stream according to claim 5 , wherein acquiring an image of droplets comprises acquiring a plurality of images of droplets with different driving voltages and wherein controlling the driving voltage comprises selecting the driving voltage for which a ratio of the length along the travel direction to the distance between the microparticle containing droplet and the fluid is in a prescribed range, and applying the selected driving voltage to the vibratory element.

Assignees

• Sony Corp

Inventors

• Muraki Yosuke

Classifications

• G01N2015/1415

  Control of particle position · CPC title

• G01N15/1404 Primary

  Handling flow, e.g. hydrodynamic focusing · CPC title

• B03C7/003

  Pretreatment of the solids prior to electrostatic separation · CPC title

• G06T7/0012

  Biomedical image inspection · CPC title

• G01N2015/1481

  Optical analysis of particles within droplets (sorting particles within droplets G01N15/1492) · CPC title