Method to predict heritable canine non-contact cruciate ligament rupture

Patent metadata

Field	Value
Publication number	US-10131950-B2
Application number	US-201615010491-A
Country	US
Kind code	B2
Filing date	Jan 29, 2016
Priority date	Jan 29, 2015
Publication date	Nov 20, 2018
Grant date	Nov 20, 2018

Abstract

Method and kits for diagnosing propensity to non-contact cranial cruciate ligament rupture (CCLR) in a dog are described. The method includes isolating genomic DNA from a dog and then analyzing the genomic DNA from step for a single nucleotide polymorphism occurring in selected loci that have been determined to be associated with the CCLR phenotype via a genome-wide association study.

First claim

What is claimed is:

1. A method for breeding a dog, comprising
a) isolating genomic DNA from a first dog;
b) assaying the genomic DNA of step (a) for one or more single nucleotide polymorphisms (SNPs) wherein the one or more SNPs comprises BICF2S23652189;
c) detecting an A allele at BICF2S23652189 in the genomic DNA of step (b); and
d) breeding the first dog having an “A” allele at BICF2S23652189.

2. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for five or more single nucleotide polymorphisms (SNPs).

3. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for ten or more single nucleotide polymorphisms (SNPs).

4. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for fifteen or more single nucleotide polymorphisms (SNPs).

5. The method of claim 1 , wherein step (b) comprises assaying the genomic DNA for twenty or more single nucleotide polymorphisms (SNPs).

6. The method of claim 1 , wherein step (b) further comprises assaying the genomic DNA of step (a) for one or more SNPs selected from the group consisting of: BICF2G630500368, BICF2P1121006, BICF2S2356299, BICF2P483191, BICF2P50610, BICF2P890246, BICF2S23324965, BICF2P544126, BICF2P526639, BICF2P1462185, BICF2P1208798, BICF2G630175389, BICF2S24415473, BICF2G630412697, BICF2P498515, BICF2P792911, BICF2G630810143, BICF2P564273, TIGRP2 P297337, BICF2G630658881, BICF2G630709791, BICF2S23147946, BICF2P181859, BICF2G630712921, BICF2G630713147, BICF2P818099, BICF2S23638642, BICF2P206910, BICF2S22959529, BICF2P1054044, BICF2S23533020, BICF2P720951, TIGRP2P31530, BICF2P247448, BICF2P1066899, BICF2G630464857, BICF2G630108404, BICF2S23148483, BICF2P866702, BICF2P1109077, BICF2P1431921, BICF2P241884, BICF2P564273, TIGRP2P46522, BICF2G630349775, BICF2S2342150, BICF2S24415473, BICF2G630359517, BICF2G63058646, BICF2P295392, BICF2G630168473, BICF2G630175389, BICF2G630810143, BICF2G630810159, BICF2G630810173, BICF2P170661, BICF2P1354767, BICF2P205255, BICF2P1358119, BICF2S23324965, BICF2S22961650, BICF2P498515, BICF2P1072682, BICF2P1090079, BCIF2P1208798, BICF2P890246, BICF2P139678, BICF2S23113199, BICF2P401973, BICF2P454456, BICF2P50610, BICF2P531097, BICF2P1290820, BICF2P65003, BICF2G630606359, BICF2S23620879, BICF2G630519882, BICF2P594418, BICF2G630422966, BICF2G630422956, BICF2G630422900, BICF2G630422895, BICF2P880005, BICF2P1121006, BICF2P888055, BICF2P582174, TIGRP2P270462, BICF2P716829, BICF2P1462185, BICF2P178583, BICF2S2328420, BICF2P420488, TIGRP2P277002, BICF2P111342, BICF2G630658881, BICF2G630658668, BICF2G630658620, BICF2G630658768, BICF2G630658756, BICF2G630658723, BICF2S2442023, BICF2S2361376, BICF2P321064,TIGRP2P293361, TIGRP2P297337, BICF2G630375268, BICF2S23730962, BICF2G630502225, BICF2G630500368, BICF2G630500363, BICF2G630500835, BICF2P544126, BICF2S24111418, BICF2G630799191, BICF2P792911, BICF2S2356299, BICF2P1332722, BICF2P1047447, BICF2P487060, BICF2P599881, BICF2P1410038,BICF2S23535135, TIGRP2P355298, BICF2S23255928, BICF2P526639,BICF2S23152419, BICF2P491441, TIGRP2P355396, BICF2P337576, BICF2P794117, BICF2P155064, BICF2P992747, BICF2S23652189, TIGRP2P362234, BICF2S23346408, 4 BICF2S23713161, BICF2S23410873, BICF2P1135545, BICF2P483191, BICF2P139173, BICF2P361907, BICF2P456086, BICF2P662502, BICF2G630412697, BICF2S2356993, BICF2P287265, BICF2S23054250, BICF2P1086740, and BICF2P708698.

7. The method of claim 6 , wherein step (b) comprises assaying the genomic DNA for five or more single nucleotide polymorphisms (SNPs).

8. The method of claim 6 , wherein step (b) comprises assaying the genomic DNA for ten or more single nucleotide polymorphisms (SNPs).

9. The method of claim 6 , wherein step (b) comprises assaying the genomic DNA for fifteen or more single nucleotide polymorphisms (SNPs).

10. The method of claim 6 , wherein step (b) comprises assaying the genomic DNA for twenty or more single nucleotide polymorphisms (SNPs).

11. The method of claim 1 , further comprising
a) isolating genomic DNA from a second dog;
b) assaying the genomic DNA from the second dog for one or more single nucleotide polymorphisms (SNPs) wherein the one or more SNPs comprises BICF2S23652189;
c) detecting an A allele at BICF2S23652189 in the genomic DNA of step (b); and
d) breeding the second dog with the first dog having an “A” allele at BICF2S23652189.

12. The method of claim 11 , wherein step (b) comprises assaying the genomic DNA of the second dog for five or more single nucleotide polymorphisms (SNPs).

13. The method of claim 11 , wherein step (b) comprises assaying the genomic DNA of the second dog for ten or more single nucleotide polymorphisms (SNPs).

14. The method of claim 11 , wherein step (b) comprises assaying the genomic DNA of the second dog for fifteen or more single nucleotide polymorphisms (SNPs).

15. The method of claim 11 , wherein step (b) comprises assaying the genomic DNA of the second dog for twenty or more single nucleotide polymorphisms (SNPs).

16. The method of claim 11 , wherein step (b) further comprises assaying the genomic DNA of the second dog for one or more SNPs selected from the group consisting of: BICF2G630500368, BICF2P1121006, BICF2S2356299, BICF2P483191, BICF2P50610, BICF2P890246, BICF2S23324965, BICF2P544126, BICF2P526639, BICF2P1462185, BICF2P1208798, BICF2G630175389, BICF2S24415473, BICF2G630412697, BICF2P498515, BICF2P792911, BICF2G630810143, BICF2P564273, TIGRP2P297337, BICF2G630658881, BICF2G630709791, BICF2S23147946, BICF2P181859, BICF2G630712921, BICF2G630713147, BICF2P818099, BICF2S23638642, BICF2P206910, BICF2S22959529, BICF2P1054044, BICF2S23533020, BICF2P720951, TIGRP2P31530, BICF2P247448,BICF2P1066899, BICF2G630464857, BICF2G630108404, BICF2S23148483,BICF2P866702, BICF2P1109077, BICF2P1431921, BICF2P241884, BICF2P564273, TIGRP2P46522, BICF2G630349775, BICF2S2342150, BICF2S24415473, BICF2G630359517, BICF2G63058646, BICF2P295392, BICF2G630168473, BICF2G630175389, BICF2G630810143, BICF2G630810159, BICF2G630810173, BICF2P170661, BICF2P1354767, BICF2P205255, BICF2P1358119, BICF2S23324965, BICF2S22961650, BICF2P498515, BICF2P1072682, BICF2P1090079, BICF2P1208798, BICF2P890246, BICF2P139678, BICF2S23113199, BICF2P401973, BICF2P454456, BICF2P50610, BICF2P531097, BICF2P1290820, BICF2P65003, BICF2G630606359, BICF2S23620879, BICF2G630519882, BICF2P594418, BICF2G630422966, BICF2G630422956, BICF2G630422900, BICF2G630422895, BICF2P880005, BICF2P1121006, BICF2P888055, BICF2P582174, TIGRP2P270462, BICF2P716829, BICF2P1462185, BICF2P178583, BICF2S2328420, BICF2P420488, TIGRP2P277002, BICF2P111342, BICF2G630658881, BICF2G630658668, BICF2G630658620, BICF2G630658768, BICF2G630658756, BICF2G630658723, BICF2S2442023, BICF2S2361376, BICF2P321064, TIGRP2P293361, TIGRP2P297337, BICF2G630375268, BICF2S23730962, BICF2G630502225, BICF2G630500368, BICF2G630500363, BICF2G630500835, BICF2P544126, BICF2S24111418, BICF2G630799191, BICF2P792911, BICF2S2356299, BICF2P1332722, BICF2P1047447, BICF2P487060, BICF2P599881, BICF2P1410038, BICF2S23535135, TIGRP2P355298, BICF2S23255928, BICF2P526639, BICF2S23152419, BICF2P491441, TIGRP2P355396, BICF2P337576, BICF2P794117, BICF2P155064, BICF2P992747, BICF2S23652189, TIGRP2P362234, BICF2S23346408, BICF2S23713161, BICF2S23410873, BICF2P1135545, BICF2P483191, BICF2P139173, BICF2P361907, BICF2P456086, BICF2P662502, BICF2G630412697, BICF2S2356993, BICF2P287265, BICF2S23054250, BICF2P1086740, and BICF2P708698.

17. The method of claim 16 , wherein step (b) comprises assaying the genomic DNA for five or more single nucleotide polymorphisms (SNPs).

18. The method of claim 16 , wherein step (b) comprises a

Assignees

• Wisconsin Alumni Res Found

Inventors

• Muir Peter
• Baker Lauren

Classifications

• C12Q2600/172

  Haplotypes · CPC title

• C12Q2600/16

  Primer sets for multiplex assays · CPC title

• C12Q2600/124

  Animal traits, i.e. production traits, including athletic performance or the like · CPC title

• C12Q2600/118

  Prognosis of disease development · CPC title

• C12Q1/6883 Primary

  for diseases caused by alterations of genetic material · CPC title