Bioactive compositions for high avidity cell capture

Patent metadata

Field	Value
Publication number	US-10131899-B2
Application number	US-201414215552-A
Country	US
Kind code	B2
Filing date	Mar 17, 2014
Priority date	Mar 17, 2014
Publication date	Nov 20, 2018
Grant date	Nov 20, 2018

Abstract

A composition useful for cell capture, the composition comprising a solid substrate on which is affixed a patterned polymer, and a cell-targeting agent attached to said patterned polymer, wherein said cell-targeting agent is exposed. Also described is a method for the preparation of the cell capturing composition, as well as flow through devices in which the cell capturing composition is incorporated. Further described is a method of capturing cells by contacting the cell-capturing composition with a liquid or gaseous sample containing cells. The method for capturing cells may also be a method for testing for the presence of one or more classes or species of cells or cellular organisms in a liquid or gaseous sample.

First claim

What is claimed is:

1. A composition useful for cell capture, the composition comprising a solid substrate on which is covalently affixed a patterned polymer having a substantially planar surface defined by x- and y-axes perpendicular to each other, and an array of regularly spaced protruded shapes on said substantially planar surface, wherein said array of regularly spaced protruded shapes has a set frequency of occurrence and a pattern density, each protruded shape being vertically raised along a z-axis perpendicular to the x- and y-axes of the substantially planar surface; having a height along the z-axis of at least 100 nm; and possessing an outer perimeter along the x- and y-axes, wherein said outer perimeter along the x- and y-axes for each protruded shape is vertically raised along the z-axis by a greater extent than portions of the z-axis of the protruded shape within the confines of the outer perimeter along the x- and y-axes, and cell-targeting agents covalently attached to said patterned polymer by a crosslinking group, wherein said cell-targeting agents are lectin and are exposed.

2. The composition of claim 1 , wherein said cell-targeting agent is a microbe-targeting agent.

3. The composition of claim 1 , wherein said patterned polymer is a block-copolymer.

4. The composition of claim 1 , wherein said patterned polymer is a vinyl addition polymer.

5. The composition of claim 1 , wherein said solid substrate is selected from the group consisting of metal, metal oxide, semiconductor, and plastic.

6. A method of capturing cells, the method comprising contacting a cell capturing composition with said cells, the cell-capturing composition comprising the composition of claim 1 .

7. The method of claim 6 , wherein said cell-targeting agent is a microbe-targeting agent.

8. The method of claim 6 , wherein said patterned polymer is a block-copolymer.

9. The method of claim 6 , wherein said patterned polymer is a vinyl addition polymer.

10. The method of claim 6 , wherein said solid substrate is selected from the group consisting of metal, metal oxide, semiconductor, and plastic.

11. The method of claim 6 , wherein said cells are microbes.

12. The method of claim 6 , wherein said cell-targeting agent is selective for a cell type.

13. The method of claim 6 , wherein said cell-targeting agent is selective for a genus or species of a microbe.

14. The method of claim 6 , wherein said cell-capturing composition is incorporated in a flow-through device.

15. The method of claim 6 , wherein said cells are within a liquid.

16. The method of claim 6 , wherein said cells are in air.

17. The composition of claim 1 , wherein said crosslinking group is a ring-opened azlactone group.

18. The composition of claim 1 , wherein each protruded shape has a height of at least 500 nm.

19. The composition of claim 1 , wherein said protruded shapes are selected from the group consisting of linear, circular, and polygonal protruded shapes.

Assignees

• Ut Battelle Llc

Inventors

• Hansen Ryan Robert
• Retterer Scott T
• Lokitz Bradley Steward
• Morrell-Falvey Jennifer L
• Salazar Juan Pablo Hinestrosa
• Messman Jamie Michael
• Kilbey Ii Sidney Michael
• Ankner John Francis

Classifications

• C08F293/00

  Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule (on to polymers modified by introduction of unsaturated end groups C08F290/02) · CPC title

• C12N11/06

  attached to the carrier via a bridging agent · CPC title

• C12N11/08 Primary

  the carrier being a synthetic polymer · CPC title

• G03F7/0002

  Lithographic processes using patterning methods other than those involving the exposure to radiation, e.g. by stamping · CPC title

• C12N11/087 Primary

  Acrylic polymers · CPC title