Antimicrobial polyamide compositions and mastitis treatment

Patent metadata

Field	Value
Publication number	US-10131744-B2
Application number	US-201414204339-A
Country	US
Kind code	B2
Filing date	Mar 11, 2014
Priority date	Mar 15, 2013
Publication date	Nov 20, 2018
Grant date	Nov 20, 2018

Abstract

The present invention relates to veterinary compositions and methods of treating and/or preventing mastitis in non-human mammals. More particularly, the present invention relates to the treatment of mastitis in cows. The veterinary composition comprises a water soluble, locally-acting, antimicrobial amine functional polyamide polymer.

First claim

What is claimed is:

1. A veterinary composition for treating infections in non-human animals, comprising a suitable veterinary carrier and at least one antimicrobial polyamide: and wherein the polyamide is present in a bactericidally effective amount, wherein the composition is effective in treating infections or disease states caused by at least one of the following pathogens selected from the group consisting of Staphylococcus spp., Streptococcus spp., Mycoplasma bovis , and E. coli , wherein the polyamide is selected from the group consisting of:

and combinations thereof.

2. The veterinary composition of claim 1 , wherein the polyamide has the following structure:

and wherein the weight average molecular weight (WAMW) is from about 1.0 kDa to about 15.0 kDa, as measured by size exclusion chromatography.

3. The veterinary composition of claim 2 , wherein the polyamide has a WAMW from about 2.0 kDa to about 10 kDa.

4. The veterinary composition of claim 2 , wherein the polyamide has a WAMW from about 2.5 kDa to about 7.76 kDa, or has a WAMW of about 7.76 kDa.

5. The veterinary composition of claim 1 , wherein one dose of the composition, for the treatment of one udder quarter, contains from about 20 to about 3000 mg of the polyamide.

6. The veterinary composition of claim 5 , wherein the dose contains from about too to about 2000 mg; from about 200 to about 1500 mg; from about 250 to about 1000 mg; from about 300 to about 500 mg; or about 300 mg of the polyamide.

7. The veterinary composition of claim 6 , wherein the dose contains from about 300 to about 500 mg of the polyamide.

8. The veterinary composition of claim 1 , wherein the veterinarily acceptable carrier comprises a thickener or rheology modifier (TRM).

9. The veterinary composition of claim 8 , wherein the TRM is selected from the group consisting of any acceptable cellulose derivative, methyl cellulose (MC), ethylcellulose (EC), EC N50, hydroxymethyl cellulose (HMC), hydroxypropyl cellulose (HPC), hydroxypropyl methyl cellulose (HPMC), hydroxyethyl cellulose (HEC), polyethylene glycols (PEGs), poloxamers, block copolymers, cross-linked acrylic acid-based polymers, carbomers, CARBOPOL polymers, alkali-swellable emulsion (ASE) polymers, polysaccharides, modified polysaccharides, modified starches, partially or pre-gelatinized starch, aluminum stearate, 12-hydroxystearin, THIXCIN, beeswax, emulsifying waxes, hydrogenated peanut oil, castor oil, hydrogenated castor oil, hard/soft paraffin, metal salts of fatty acids, mucoadhesives, alkyl triammonium methosulfate, ceteraryl octanoate, polyvinyl alcohol, chitosan, chitosan derivatives, trimethylated chitosan, xanthum gum, guar gum, hyaluronic acid, thermo-gelling agents, shear-thinning agents, shear-gelling agents, polycarbophil, polyethylene oxide, silica, fumed silica, any fumed metal oxide, non-toxic heavy metal salts, hydrogenated oils, hydrogenated castor oil and combinations thereof.

10. The veterinary composition of claim 9 , wherein the TRM is a cellulose derivative selected from the group consisting of methyl cellulose (MC), ethylcellulose (EC), EC N50, hydroxymethyl cellulose (HMC), hydroxypropyl cellulose (HPC), hydroxypropyl methyl cellulose (HPMC), hydroxyethyl cellulose (HEC), and combinations thereof.

11. The veterinary composition of claim 10 , wherein the composition has a viscosity (measured at 20° C.) from about 200 cP to about 8,000 cP or from about 4,000 cP to about 6,000 cP; and wherein the viscosity is measured using a spindle viscometer.

12. The veterinary composition of claim 11 , wherein the TRM is HPMC having a WAMW of about 86 kDa, a methoxyl content of about 28 to about 30% and a hydroxypropoxyl content of about 7 to about 12% of the HPMC; and wherein the HPMC has the CAS number 9004-65-3.

13. The veterinary composition of claim 12 , wherein the viscosity decreases when the temperature increases from about 20° C. to about 33° C., or to about the temperature of a lactating animal's udder.

14. The veterinary composition of claim 12 , wherein the viscosity is about 4,000 to 5,000 Cp (at 20° C.); about 3,000 to about 4,000 (at 25° C.); and about 2,000 to about 3,000 (at 33° C.).

15. The veterinary composition of claim 12 , wherein the viscosity is about 1300 to about 1500 (at 20° C.); about 900 to about 1,200 (at 25° C.); and about 600 to about 800 (at 33° C.).

16. The veterinary composition of claim 11 or 12 , wherein the TRM is present in amount of about 1 to about 5% w/v of the composition.

17. The veterinary composition of claim 9 , wherein the TRM is one that causes the composition to increase viscosity when the temperature is increased from about 20° C. to about 33° C., or to about the temperature of a lactating animal's udder.

18. The veterinary composition of claim 17 , wherein the TRM is a poloxamer.

19. The veterinary composition of claim 1 , wherein the composition is a paste.

20. The veterinary composition of claim 19 , comprising the antimicrobial polyamide, a gel base, and a non-toxic heavy metal salt.

21. The veterinary composition of claim 20 , wherein the gel base comprise liquid paraffin and the heavy metal salt comprises bismuth subnitrate.

22. The veterinary composition of claim 19 , which comprises an antioxidant selected from the group consisting of alpha tocopherol, ascorbic acid, ascorbyl palmitate, fumaric acid, malic acid, sodium ascorbate, sodium metabisulfate, n-propyl gallate, BHA, BHT and monothioglycerol.

23. The veterinary composition of claim 19 , which comprises a preservative selected from the group consisting of parabens, benzalkonium chloride, benzethonium chloride, benzoic acid, benzyl alcohol, bronopol, cetrimide, chlorhexidine, chlorobutanol, chlorocresol, cresol, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric acetate, phenylmercuric borate, phenylmercuric nitrate, potassium sorbate, sodium benzoate, sodium propionate, sorbic acid, and thimerosal.

Assignees

• Merial Ltd
• Genzyme Corp
• Merial Inc

Inventors

• Cady Susan Mancini
• Galeska Izabela
• Dhal Pradeep K

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61P31/00

  Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics · CPC title

• A61P1/00

  Drugs for disorders of the alimentary tract or the digestive system · CPC title

• A61P1/02

  Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis · CPC title