Bipodal-peptide binder

Patent metadata

Field	Value
Publication number	US-10131693-B2
Application number	US-99971709-A
Country	US
Kind code	B2
Filing date	Oct 20, 2009
Priority date	Oct 20, 2008
Publication date	Nov 20, 2018
Grant date	Nov 20, 2018

Abstract

The present invention deals with a bipodal-peptide binder that specifically binds with a target including (a) a structure stabilizing region that includes parallel, antiparallel or parallel and antiparallel amino acid strands wherein interstrand non-covalent bonds are formed; and (b) a target binding region I and a target binding region II that are bonded at both terminals of said structure stabilizing region and respectively include n and m amino acids, and a method of preparing same; the bipodal-peptide binder of the present invention exhibits the KD value (dissociation constant) of a very low level (for example, nM level) and, therefore, exhibits very high affinity toward a target. The bipodal-peptide binder of the present invention has applications not only in pharmaceuticals but also in in-vivo imaging, in vitro cell imaging, and drug delivery targeting, and can be very usefully employed as an escort molecule.

First claim

What is claimed is:

1. A bipodal peptide binder which specifically binds to a target, comprising:
(a) a tryptophan zipper motif as a structure stabilizing region; and
(b) a target binding region I linked to one terminus of the tryptophan zipper motif and a target binding region II linked to the other terminus of the tryptophan zipper motif, wherein the target binding region I and the target binding region II are covalently linked through the structure stabilizing region and are each a peptide that has binding affinity for the target, and wherein the number of amino acid residues of each of target binding region I and target binding region II is an integer of 2-50,
wherein the amino acid sequence of said bipodal peptide binder is non-naturally occurring.

2. The bipodal peptide binder according to claim 1 , wherein the tryptophan zipper motif comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-3 and 5-10.

3. The bipodal peptide binder according to claim 1 , wherein the tryptophan zipper motif is represented by the following Formula I:
X 1 -Trp(X 2 )X 3 -X 4 -X 5 (X′ 2 )X 6 -X 7   Formula I

wherein X 1 represents Ser or Gly-Glu, and X 2 and X′ 2 independently represent Thr, His, Val, Ile, Phe or Tyr, and X 3 represents Trp or Tyr, and X 4 represents type I, type I′, type II, type II′, type III or type III′ turn sequence, and X 5 represents Trp or Phe, and X 6 represents Trp or Val, and X 7 represents Lys or Thr-Glu.

4. The bipodal peptide binder according to claim 3 , wherein the tryptophan zipper motif is represented by the Formula I in which X 1 represents Ser or Gly-Glu, and X 2 and X′ 2 independently represent Thr, His or Val, and X 3 represents Trp or Tyr, and X 4 represents type I, type I′, type II or type II′ turn sequence, and X 5 represents Trp or Phe, and X 6 represents Trp or Val, and X 7 represents Lys or Thr-Glu.

5. The bipodal peptide binder according to claim 1 , wherein the number of amino acid residues of each of the target binding region I and the target binding region II is an integer of 2-20.

6. The bipodal peptide binder according to claim 1 , wherein the target binding region I and the target binding region II bind in a cooperative manner to the target.

7. The bipodal peptide binder according to claim 1 , wherein the structure stabilizing region, the target binding region I or the target binding region II further comprises a functional molecule.

8. The bipodal peptide binder according to claim 7 , wherein the functional molecule comprises a label capable of generating a detectable signal, a chemical drug, a biodrug, a cell penetrating peptide (CPP) or a nanoparticle.

9. The bipodal peptide binder according to claim 1 , wherein the target comprises a biochemical material, a peptide, a polypeptide, a nucleic acid, a carbohydrate, a lipid, a cell, a tissue, a compound, a metal material or a non-metal material.

10. The bipodal peptide binder according to claim 1 , wherein the number of amino acid residues of each of the target binding region I and the target binding region II is an integer of 3-10.

Assignees

• Jon Sang Yong
• Kim Sung Hyun
• Park Se Ho
• Gwangju Inst Science & Tech

Inventors

• Jon Sang Yong
• Kim Sung Hyun
• Park Se Ho

Classifications

• A61K49/0056

  Peptides, proteins, polyamino acids · CPC title

• A61K49/0032

  Methine dyes, e.g. cyanine dyes · CPC title

• C07K14/001 Primary

  by chemical synthesis · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title

• C07K7/06 Primary

  having 5 to 11 amino acids · CPC title