System and method for ionization of molecules for mass spectrometry and ion mobility spectrometry

Patent metadata

Field	Value
Publication number	US-10128096-B2
Application number	US-201715401253-A
Country	US
Kind code	B2
Filing date	Jan 9, 2017
Priority date	Sep 2, 2010
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

An ionizing system includes a channel and a heater coupled to the channel. The channel has an inlet disposed in a first pressure region having a first pressure and an outlet disposed in a second pressure region having a second pressure. The first pressure is greater than the second pressure. The heater is for heating the channel, and the channel is configured to generate charged particles of a sample in response to the sample being introduced into the channel.

First claim

What is claimed is:

1. An ionizing system, comprising:
a tube defining a channel, the tube having a first end, which defines an inlet of the channel, disposed in a first pressure region having a first pressure and a second end, which defines an outlet of the channel, disposed in a second pressure region having a second pressure, the first pressure being greater than the second pressure, wherein the inlet is configured to allow passage into the channel of a neutral analyte sample; and
a heater coupled to the tube for heating the channel within the tube,
wherein the channel is configured to facilitate the generation of a charged analyte sample including at least one of a protonated ion or a metal cationized ion of an analyte molecule from the neutral analyte sample in response to the neutral analyte sample being passed through the inlet and into the channel, wherein generation of the charged analyte sample is due to a pressure differential across the channel and due to heat from the heater.

2. The ionizing system of claim 1 , wherein the neutral analyte sample includes an analyte disposed within a matrix prior to being introduced into the channel.

3. The ionizing system of claim 2 , wherein the matrix includes a solid.

4. The ionizing system of claim 2 , wherein the matrix includes a solvent, and the solvent includes an effluent from a liquid separation device.

5. The ionizing system of claim 4 , wherein the effluent is introduced into the channel via a flow of at least one of nanoliters per minute and microliters per minute.

6. The ionizing system of claim 2 , further comprising a device for introducing the neutral analyte sample into the channel, the device including:
a container including the solvent in which the analyte is dissolved; and
a conduit having a first end disposed in the container such that the first end is in contact with the analyte dissolved in the solvent and a second end is disposed within the channel adjacent to the inlet of the channel.

7. The ionizing system of claim 1 , further comprising a device for introducing the neutral analyte sample into the channel by way of applying a force to the neutral analyte sample.

8. The ionizing system of claim 7 , wherein the device includes a laser configured to ablate the neutral analyte sample.

9. The ionizing system of claim 7 , wherein the device includes an ultrasonic probe.

10. The ionizing system of claim 7 , wherein the force is a piezoelectric force.

11. The ionizing system of claim 7 , wherein the device introduces the neutral analyte sample directly into the channel.

12. The ionizing system of claim 1 , wherein the tube is a transfer tube that is coupled to the heater such that the transfer tube is heated by the heater.

13. The ionizing system of claim 1 , wherein the tube is part of a heater such that the channel is defined by the heater.

14. The ionizing system of claim 1 , wherein a skimmer is disposed over the inlet of the channel and defines an opening that is in fluid communication with the channel.

15. The ionizing system of claim 1 , wherein the neutral analyte sample includes an analyte incorporated in a matrix prior to being introduced into the channel.

16. The ionizing system of claim 15 , wherein gas-phase analyte ions are generated upon loss of molecules of the matrix from the charged particles of the charged analyte sample.

17. A method, comprising:
creating a pressure differential across a channel;
heating the channel; and
generating a charged analyte sample including at least one of a protonated ion or a metal cationized ion of an analyte molecule from the neutral analyte sample in response to the neutral analyte sample being passed through an inlet and into the channel, wherein the charged analyte sample is generated due to heat within the channel and due to the pressure differential across the channel.

18. The method of claim 17 , wherein the neutral analyte sample is disposed in a solvent prior to being received in the channel.

19. The method of claim 16 , wherein the neutral analyte sample is incorporated into a matrix prior to being received in the channel.

20. The method of claim 19 , further comprising generating gas-phase analyte ions upon loss of neutral molecules of the matrix from charged particles of the charged analyte sample.

21. The method of claim 18 , wherein gas-phase analyte ions are generated in response to ions of the charged analyte sample contacting an obstruction disposed along an axis of the channel.

22. The method of claim 17 , wherein the neutral analyte sample includes at least one analyte molecule disposed within an aerosol droplet.

23. The method of claim 17 , wherein at least one of a multiply charged ion and a singly charged ion of the sample are generated in the channel.

24. The method of claim 17 , wherein the neutral analyte sample includes at least one of drugs, peptides, proteins, and tissue.

Assignees

• Univ Of The Sciences In Philadelphia
• Univ Wayne State

Inventors

• Trimpin Sarah
• Mcewen Charles Nehemiah
• Pagnotti Vincent Salvatore

Classifications

• H01J49/10 Primary

  Ion sources; Ion guns · CPC title

• H01J49/0468

  with means for heating or cooling the sample · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title