High numerical aperture telemicroscopy apparatus

Patent metadata

Field	Value
Publication number	US-10126539-B2
Application number	US-201615346923-A
Country	US
Kind code	B2
Filing date	Nov 9, 2016
Priority date	Jan 2, 2008
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

An imaging system consisting of a cell-phone with camera as the detection part of an optical train which includes other components. Optionally, an illumination system to create controlled contrast in the sample. Uses include but are not limited to disease diagnosis, symptom analysis, and post-procedure monitoring, and other applications to humans, animals, and plants.

First claim

What is claimed is:

1. A microscopy imaging apparatus, the apparatus comprising:
a microscope housing removably attachable to a wireless communications device having a camera comprising one or more device lenses and an image sensor; and
a microscope lens system mounted to the microscope housing, wherein the microscope lens system comprises a microscope lens that is aligned with the camera when the microscope housing is attached to the wireless communications device;
wherein the microscope housing positions the microscope lens system in alignment with an image sensor of the camera such that an image of a sample is projected onto the image sensor at a suitable numerical aperture (NA) and magnification (M) using a combination of the microscope lens system and one or more device lenses of the camera;
wherein the microscope lens system has a collection NA of at least approximately 0.01 and a M of at least about 1.

2. The microscopy imaging apparatus of claim 1 , wherein M≥4.

3. The microscopy imaging apparatus of claim 1 , further comprising a sample holder.

4. The microscopy imaging apparatus of claim 3 , wherein the sample holder comprises a microfluidic device or other small-volume fluid handling system that is removable from the sample holder.

5. The microscopy imaging apparatus of claim 3 , wherein the sample holder and comprises a stage allowing for indexed translation in x-y-z directions to allow sample multiple fields of view.

6. The microscopy imaging apparatus of claim 3 , wherein the sample holder and microscope lens system comprise a detection arm of a fluorescence assay.

7. The microscopy imaging apparatus of claim 6 , wherein the fluorescence assay configured for illumination comprising one or more of phase contrast, bright field, epi-illumination, or dark field microscopy.

8. The microscopy imaging apparatus of claim 6 , wherein the fluorescence assay comprises an emission filter associated with a Color Filter Array (CFA) of the camera.

9. The microscopy imaging apparatus of claim 1 , wherein the microscope lens system is telecentric on an object side, on an image side, or on both sides.

10. The microscopy imaging apparatus of claim 1 , further comprising a contrast selected from transillumination brightfield, darkfield, phase contrast, differential interference contrast, polarization contrast, Hoffman modulation contrast, fluorescence, Schlieren, and oblique transillumination.

11. The microscopy imaging apparatus of claim 1 , further comprising an illumination source coupled to the microscope lens system.

12. The microscopy imaging apparatus of claim 11 , wherein the illumination source comprises a LED for fluorescence excitation.

13. The microscopy imaging apparatus of claim 12 , wherein the illumination source comprises a monochromatic LED.

14. The microscopy imaging apparatus of claim 1 , wherein the microscope housing is configured to snap onto said communications device.

15. The microscopy imaging apparatus of claim 1 , wherein the microscope lens system is wirelessly coupled to the wireless communications device for at least one microscopy mode.

16. The microscopy imaging apparatus of claim 1 , wherein the apparatus is configured for scaling an image of received from the microscope lens system.

17. The microscopy imaging apparatus of claim 1 , wherein the microscope lens system comprises a high resolution condenser.

18. The microscopy imaging apparatus of claim 1 , wherein the apparatus is configured for wireless connection to a remote device for one or more of image upload and processing.

19. The microscopy imaging apparatus of claim 1 , wherein the microscope lens system comprises a zoom lens to adjust field of view.

20. The microscopy imaging apparatus of claim 19 , wherein the zoom lens is adjustable.

21. The microscopy imaging apparatus of claim 1 , wherein the apparatus is configured for imaging samples comprising skin or wounds.

Assignees

• Univ California

Inventors

• Fletcher Daniel
• Hansen Wendy
• Switz Neil
• Breslauer David N
• Douglas Erik
• Maamari Robi
• Lam Wilbur
• Dill Jesse

Classifications

• G01N21/8483

  Investigating reagent band (test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04) · CPC title

• G02B21/0092

  Polarisation microscopes · CPC title

• G02B21/36

  arranged for photographic purposes or projection purposes (G02B21/18 takes precedence){or digital imaging or video purposes including associated control and data processing arrangements (image data processing per se G06T)} · CPC title

• G02B21/16

  adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} · CPC title

• G02B21/365

  Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title