Composition for preventing or treating atopic dermatitis comprising X-shape DNA structure as active ingredient

Patent metadata

Field	Value
Publication number	US-10125367-B2
Application number	US-201615562894-A
Country	US
Kind code	B2
Filing date	Apr 6, 2016
Priority date	Apr 7, 2015
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating atopic dermatitis, the pharmaceutical composition including, as an active ingredient, X-shaped DNA (X L -DNA) formed by complementary binding of oligonucleotides having nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 4. When the pharmaceutical composition is subcutaneously injected into an animal model of atopic dermatitis, effects of easing skin lesions, such as erythema, bleeding and edema, and the like, and ear edema, and reducing expression of immunoglobulin E (IgE) are exhibited. In this regard, the composition can be used as a pharmaceutical composition, a health food, or a cosmetic for atopic dermatitis.

First claim

The invention claimed is:

1. A method of preventing or treating atopic dermatitis in a subject in need thereof, the method comprising:
providing a pharmaceutical composition comprising X-shaped DNA (X L -DNA) formed by complementary binding of oligonucleotides having the nucleotide sequence of SEQ ID NO: 1 to SEQ ID NO: 4 as an active ingredient; and
administering the pharmaceutical composition to the subject, wherein the atopic dermatitis is prevented or treated.

2. The method of claim 1 , wherein the X L -DNA forms a polymer by complementary binding between two or more X L -DNAs.

3. The method of claim 2 , wherein the X L -DNA inhibits production of immunoglobulin E (IgE).

4. The method of claim 1 , wherein the X L -DNA reduces expression of thymic stromal lymphopoietin (TSLP) or thymus and activation-regulated chemokine (TARC).

5. A health food for preventing or treating atopic dermatitis, the health food comprising, as an active ingredient, X-shaped DNA formed by complementary binding of oligonucleotides having nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 4.

6. The health food of claim 5 , wherein the X-shaped DNA forms a polymer by complementary binding between two or more X L -DNAs.

7. A method of preventing or treating atopic dermatitis in a subject in need thereof, the method comprising:
providing a cosmetic composition comprising X-shaped DNA formed by complementary binding of oligonucleotides having the nucleotide sequence of SEQ ID NO: 1 to SEQ ID NO: 4 as an active ingredient; and
administering the cosmetic composition to the subject, wherein the atopic dermatitis is prevented or treated.

8. The method of claim 7 , wherein the X-shaped DNA forms a polymer by complementary binding between two or more X L -DNAs.

Assignees

• Catholic Univ Korea Ind Academic Cooperation Foundation

Inventors

• Lee Joo Young
• Yang Gabsik

Classifications

• C12N2310/17

  Immunomodulatory nucleic acids · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title

• C12N15/117 Primary

  Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs · CPC title

• A61K8/606

  Nucleosides; Nucleotides; Nucleic acids · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title