Bioreactor sterilization method for multiple uses

Patent metadata

Field	Value
Publication number	US-10125346-B2
Application number	US-201615143012-A
Country	US
Kind code	B2
Filing date	Apr 29, 2016
Priority date	Mar 31, 2015
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

Some embodiments include a method. The method can include: conducting a first sterilization of an assembled bioreactor; after conducting the first sterilization of the assembled bioreactor, vitally supporting one or more first microorganisms with the assembled bioreactor; after vitally supporting the one or more first microorganisms with the assembled bioreactor, removing the one or more first microorganisms from the assembled bioreactor; after removing the one or more first microorganisms from the assembled bioreactor, gathering up the assembled bioreactor; after gathering up the assembled bioreactor, conducting a second sterilization of the assembled bioreactor; and after conducting the second sterilization of the assembled bioreactor, vitally supporting one or more second microorganisms with the assembled bioreactor. Other embodiments of related systems and methods are also disclosed.

First claim

What is claimed is:

1. A method comprising:
conducting a first sterilization of an assembled bioreactor, wherein sterilizing the assembled bioreactor comprises at least one of gamma irradiating the assembled bioreactor or autoclaving the assembled bioreactor;
after conducting the first sterilization of the assembled bioreactor, vitally supporting one or more first microorganisms with the assembled bioreactor;
after vitally supporting the one or more first microorganisms with the assembled bioreactor, removing the one or more first microorganisms from the assembled bioreactor;
after removing the one or more first microorganisms from the assembled bioreactor, applying water to the bioreactor and gathering up the assembled bioreactor, wherein gathering up the assembled bioreactor comprises at least one of folding or rolling up the assembled bioreactor after removing the one or more first microorganisms from the assembled bioreactor;
after gathering up the assembled bioreactor, conducting a second sterilization of the assembled bioreactor, wherein the second sterilization of the assembled bioreactor comprises autoclaving the bioreactor containing water; and
after conducting the second sterilization of the assembled bioreactor, vitally supporting one or more second microorganisms with the assembled bioreactor.

2. The method of claim 1 further comprising:
gathering up the assembled bioreactor before conducting the first sterilization of the assembled bioreactor, wherein gathering up the assembled bioreactor before conducting the first sterilization of the assembled bioreactor comprises at least one of folding the assembled bioreactor before sterilizing the bioreactor or rolling up the bioreactor before sterilizing the assembled bioreactor.

3. The method of claim 1 wherein:
vitally supporting the one or more first microorganisms with the assembled bioreactor comprises at least one of:
supplying light to the one or more first microorganisms; or
supplying organic carbon material to the one or more first microorganisms.

4. The method of claim 1 wherein:
vitally supporting the one or more first microorganisms with the assembled bioreactor comprises:
mixing the one or more first microorganisms within a fluidic support medium by injecting gas into the fluidic support medium; and
the gas comprises gas bubbles comprising a diameter greater than or equal to approximately 40 micrometers and less than or equal to approximately 2 millimeters.

5. The method of claim 1 wherein:
vitally supporting the one or more first microorganisms with the assembled bioreactor comprises:
mixing the one or more first microorganisms within a fluidic support medium by injecting gas into the fluidic support medium at a volumetric flow rate of greater than or equal to approximately 10 liters per minute and less than or equal to approximately 60 liters per minute.

6. The method of claim 1 wherein:
the assembled bioreactor comprises at least one of one or more bioreactor fittings, one or more flexible tubes, and one or more gas delivery devices.

7. The method of claim 1 wherein:
vitally supporting one or more first microorganisms with the assembled bioreactor further comprising operating the assembled bioreactor in axenic conditions.

8. The method of claim 7 wherein:
vitally supporting one or more second microorganisms with the assembled bioreactor further comprising operating the assembled bioreactor in the axenic conditions.

9. The method of claim 1 wherein:
the one or more first microorganisms and the one or more second microorganisms comprise at least one of microalgae or cyanobacteria.

10. The method of claim 1 wherein:
the one or more first microorganisms and the one or more second microorganisms comprise at least one of one or more phototrophic microorganisms, one or more mixotrophic microorganisms, or one or more heterotrophic microorganisms.

11. The method of claim 1 wherein:
the one or more first microorganisms and the one or more second microorganisms comprise one or more microalgae; and
the one or more microalgae are taxonomically classified in at least one of taxonomic family Chlorellaceae, taxonomic family Haematococcaceae, taxonomic family Scenedesmaceae, taxonomic family Porphyridiaceae, or taxonomic family Chlamydomonadaceae.

12. The method of claim 1 wherein:
the assembled bioreactor further comprises a greatest physical dimension; and
the assembled bioreactor is configured to be the at least one of folded up or rolled up such that the greatest physical dimension is reducible by at least approximately 75%.

13. A method comprising:
gathering up an assembled bioreactor before conducting a first sterilization of the assembled bioreactor, wherein gathering up the assembled bioreactor before conducting the first sterilization of the assembled bioreactor comprises at least one of folding the assembled bioreactor before sterilizing the bioreactor or rolling up the bioreactor before sterilizing the assembled bioreactor;
conducting the first sterilization of the assembled bioreactor, wherein sterilizing the assembled bioreactor comprises at least one of gamma irradiating the assembled bioreactor or autoclaving the assembled bioreactor;
after conducting the first sterilization of the assembled bioreactor, vitally supporting one or more first microorganisms with the assembled bioreactor;
after vitally supporting the one or more first microorganisms with the assembled bioreactor, removing the one or more first microorganisms from the assembled bioreactor;
after removing the one or more first microorganisms from the assembled bioreactor, applying water to the bioreactor and gathering up the assembled bioreactor, wherein gathering up the assembled bioreactor comprises at least one of folding or rolling up the assembled bioreactor after removing the one or more first microorganisms from the assembled bioreactor;
after gathering up the assembled bioreactor, conducting a second sterilization of the assembled bioreactor, wherein the second sterilization of the assembled bioreactor comprises autoclaving the bioreactor containing water; and
after conducting the second sterilization of the assembled bioreactor, vitally supporting one or more second microorganisms with the assembled bioreactor;
wherein:
the assembled bioreactor further comprises a greatest physical dimension; and
the assembled bioreactor is configured to be the at least one of folded up or rolled up such that the greatest physical dimension is reducible by at least approximately 75%.

14. The method of claim 13 wherein:
vitally supporting the one or more first microorganisms with the assembled bioreactor comprises at least one of:
supplying light to the one or more first microorganisms; or
supplying organic carbon material to the one or more first microorganisms.

15. The method of claim 14 wherein:
vitally supporting the one or more first microorganisms with the assembled bioreactor comprises:
mixing the one or more first microorganisms within a fluidic support medium by injecting gas into the fluidic support medium; and
the gas comprises gas bubbles comprising a diameter greater than or equal to approximately 40 micrometers and less than or equal to approximately 2 millimeters.

16. The method of claim 14 wherein:
vitally supporting the one or more first microorganisms with the assembled bioreactor comprises:
mixing the one or more first microorganisms within a fluidic support medium by injecting gas into the fluidic support medium at a volumetric flow rate of greater than or equal to approximate

Assignees

• Heliae Dev Llc
• Heliae Dev Llc

Inventors

• Cizek Luke
• Oelschlager Mason
• Hayden Justin
• Mccarty Mason

Classifications

• A61L2/081

  Gamma radiation · CPC title

• C12M41/22

  in contact with the bioreactor walls · CPC title

• C12M21/02 Primary

  Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12) · CPC title

• A61L2/07

  Steam · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title