Droplet fluid connections

Patent metadata

Field	Value
Publication number	US-10125342-B2
Application number	US-201615248509-A
Country	US
Kind code	B2
Filing date	Aug 26, 2016
Priority date	Aug 26, 2015
Publication date	Nov 13, 2018
Grant date	Nov 13, 2018

Abstract

Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.

First claim

The invention claimed is:

1. A system, comprising:
a) a fluid source in fluidic communication with a first fluid port positioned on a first mating surface, said first fluid port comprising a first protruding fluid droplet;
b) a microfluidic device comprising a microchannel in fluidic communication with a second fluid port on a second mating surface, said second fluid port comprising a second protruding fluid droplet; and
c) a mechanism configured for bringing said first protruding fluid droplet and said second fluid droplet together in a droplet-to-droplet connection by lowering or lifting either one of said mating surfaces into contact into the other one of said mating surfaces, so that fluid can flow from said fluid source through said first fluid port into said second fluid port of said microfluidic device.

2. The system of claim 1 , wherein the first protruding fluid droplet protrudes downward from said first mating surface and said second protruding fluid droplet protrudes upward from said second mating surface.

3. The system of claim 1 , wherein the first protruding fluid droplet protrudes upward from said first mating surface and said second protruding fluid droplet protrudes downward from said second mating surface.

4. The system of claim 1 , wherein said mechanism lifts the second mating surface upward into contact with said first mating surface.

5. The system of claim 1 , wherein said mechanism lifts the first mating surface upward into contact with said second mating surface.

6. The system of claim 1 , wherein said mechanism lowers the second mating surface into contact with said first mating surface.

7. The system of claim 1 , wherein said mechanism lowers the first mating surface into contact with said second mating surface.

8. The system of claim 1 , wherein said first mating surface comprises a region surrounding said first fluid port, and wherein said region is adapted to resist wetting by said fluid.

9. The system of claim 8 , wherein said region is adapted to be hydrophobic.

10. The system of claim 9 , wherein said region comprises a first material selected to resist wetting by said fluid.

11. The system of claim 10 , wherein the first material is selected from the group consisting of poly-tetrafluoroethylene (PTFE), a perfluoroalkoxy alkane (PFA), fluorinated ethylenepropylene (FEP), polydimethylsiloxane (PDMS), nylon, polypropylene, polystyrene and polyimide.

12. The system of claim 11 , wherein said first material is bonded, adhered, coated or sputtered onto said first mating surface.

13. The system of claim 10 , wherein said first material comprises a hydrophobic gasket.

14. The system of claim 8 , wherein said region of said first mating surface is adapted to resist wetting by means of plasma treatment, ion treatment, gas-phase deposition, liquid-phase deposition, adsorption, absorption or chemical reaction with one or more agents.

15. The system of claim 1 , wherein said first mating surface comprises a region surrounding said first fluid port, and wherein said region is adapted to promote wetting by said fluid.

16. The system of claim 15 , wherein said region is adapted to be hydrophilic.

17. The system of claim 15 , wherein said region comprises a first material selected to promote wetting by said first liquid.

18. The system of claim 17 , wherein the first material is selected from the group consisting of polymethylmethacrylate (PMMA), polyvinyl alcohol (PVOH), polycarbonate (PC), polyether ether ketone (PEEK), polyethylene terephthalate (PET), polyfulfone, polystyrene, polyvinyl acetate (PVA), nylon, polyvinyl fluoride (PVF), polyvinylidiene chloride (PVDC), polyvinyl chloride (PVC) and acrylonitrile-butadiene-styrene (ABS).

19. The system of claim 18 , wherein said first material is bonded, adhered, coated or sputtered onto said first mating surface.

20. The system of claim 17 , wherein said first material comprises a hydrophilic gasket.

21. The system of claim 15 , wherein said region of said first mating surface is adapted to promote wetting by means of plasma treatment, ion treatment, gas-phase deposition, liquid-phase deposition, adsorption, absorption or chemical reaction with one or more agents.

22. The system of claim 1 , wherein the first mating surface comprises one or more ridges surrounding said first fluid ports.

23. The system of claim 1 , wherein the first mating surface comprises one or more recesses surrounding said first fluid port.

24. The system of claim 1 , wherein said first mating surface is adapted to stably retain an aqueous protruding fluid droplet.

25. The system of claim 1 , wherein said first mating surface is adapted to stably retain a non-aqueous protruding fluid droplet.

26. The system of claim 1 , wherein said first mating surface is adapted to stably retain an oil protruding droplet.

27. A method of merging droplets, comprising:
a) providing
i) a fluid source in fluidic communication with a first fluid port positioned on a first mating surface, said first fluid port comprising a first protruding fluid droplet; and
ii) a microfluidic device comprising a micro channel in fluidic communication with a second fluid port on a second mating surface, said second fluid port comprising a second protruding fluid droplet; and

b) bringing said first protruding fluid droplet and said second fluid droplet together in a droplet-to-droplet connection by lifting or lowering either one of said mating surfaces into contact with the other one of said mating surfaces, whereby the first and second fluid droplets merge so that fluid flows from said fluid source through said first fluid port into said second fluid port of said microfluidic device.

28. The method of claim 27 , wherein the first protruding fluid droplet protrudes downward from said first mating surface and said second protruding fluid droplet protrudes upward from said second mating surface.

29. The method of claim 27 , wherein the first protruding fluid droplet protrudes upward from said first mating surface and said second protruding fluid droplet protrudes downward from said second mating surface.

30. The method of claim 27 , wherein step b) comprising lifting the second mating surface upward into contact with said first mating surface.

31. The method of claim 27 , wherein step b) comprises lifting the first mating surface upward into contact with said second mating surface.

32. The method of claim 27 , wherein step b) comprising lowering the second mating surface into contact with said first mating surface.

33. The method of claim 27 , wherein step b) comprises lowering the first mating surface into contact with said second mating surface.

34. The method of claim 27 , wherein said droplet-to-droplet connection does not permit air to enter said fluid inlet port.

35. The method of claim 27 , wherein said first mating surface comprises a region surrounding said first fluid port, wherein said region is adapted to promote wetting by said fluid.

36. The method of claim 35 , wherein said region is adapted to be hydrophilic.

37. The method of claim 35 , wherein said region comprises a first material selected to promote wetting by said fluid.

38. The method of

Assignees

• Emulate Inc

Inventors

• Levner Daniel
• Sliz Josiah Daniel
• Hinojosa Christopher David
• Thompson Ii Guy Robert
• Martinus van Ruijven Petrus Wilhelmus
• Solomon Matthew Daniel
• Potzner Christian Alexander
• Tuohy Patrick Sean

Classifications

• B01L2300/0681

  Filter · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• C12N2521/00

  Culture process characterised by the use of hydrostatic pressure, flow or shear forces · CPC title

• C12M23/40 Primary

  Manifolds; Distribution pieces (fluid transfer means B01L3/563) · CPC title

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title