Detection apparatus for differential-charged particle mobility analyzer

Patent metadata

Field	Value
Publication number	US-10119971-B2
Application number	US-201615147701-A
Country	US
Kind code	B2
Filing date	May 5, 2016
Priority date	Aug 7, 2008
Publication date	Nov 6, 2018
Grant date	Nov 6, 2018

Abstract

The present invention provides devices and methods for identification and/or quantitation of particles through detection of fluorescence labeled particles in an apparatus for differential charged particle mobility analysis and fluorescence detection.

First claim

What is claimed is:

1. An apparatus for differential-charged particle mobility analysis and detection of fluorescence, the apparatus comprising:
one or more pumps adapted to transport through a capillary;
an ionizer adapted to charge particles of a fluorophore labeled sample as the sample flows within a capillary;
a differential-charged particle mobility analyzer adapted to:
receive a sample of fluorophore labeled charged particles from an ionizer; and
perform a differential-charged particle mobility analysis on a sample of charged particles; and

a fluorescence detection system adapted to detect fluorescence of the charged particles.

2. The apparatus according to claim 1 , further comprising an autosampler adapted to provide a sample for differential-charged particle mobility analysis to the one or more pumps.

3. The apparatus according to claim 1 , wherein the fluorescence detection system comprises one or more excitation sources and one or more fluorescence detectors.

4. The apparatus according to claim 3 , wherein the one or more excitation sources comprise at least one laser excitation source.

5. The apparatus according to claim 4 , wherein at least one laser excitation source operates in pulse mode.

6. The apparatus according to claim 4 , wherein at least one laser excitation source operates in continuous mode.

7. The apparatus according to claim 1 , wherein the fluorescence detection system comprises a single excitation source and a single fluorescence detector positioned on either side of a flow of the sample of charged particles.

8. The apparatus according to claim 1 , wherein the fluorescence detection system comprises an excitation source and multiple fluorescence detectors.

9. The apparatus according to claim 8 , wherein the excitation source and fluorescence detectors are arranged in an annular array positioned around a flow of the sample of charged particles.

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Caulfield Michael P

Classifications

• G01N33/92

  involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title

• G01N27/624

  Differential mobility spectrometry [DMS]; Field asymmetric-waveform ion mobility spectrometry [FAIMS] · CPC title

• G01N31/00

  Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods · CPC title

• G01N2201/06113

  Coherent sources; lasers · CPC title

• G01N33/582 Primary

  with fluorescent label · CPC title