Delivery system

Patent metadata

Field	Value
Publication number	US-10118005-B2
Application number	US-201314019714-A
Country	US
Kind code	B2
Filing date	Sep 6, 2013
Priority date	Mar 7, 2011
Publication date	Nov 6, 2018
Grant date	Nov 6, 2018

Abstract

Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.

First claim

That which is claimed is:

1. A method of dispensing a composition onto a bodily surface of a patient in need thereof, comprising:
(a) detecting said bodily surface with an optical detector to obtain three-dimensional coordinates thereof, wherein said optical detector further comprises a depth detector to account for movement of the patient;
(b) processing the three-dimensional coordinates by:
(i) interpreting the coordinates to form a model of the bodily surface;
(ii) transforming the model into a negative mold of the bodily surface, which mold is split into a plurality of Z-axis layers; and
(iii) overlaying each of said Z-axis layers in the negative mold with a series of lines which represent coverage of the bodily surface, wherein said lines provide a path for a dispenser; and

(c) dispensing the composition onto said bodily surface of said patient with said dispenser based upon said coordinates, wherein the dispensing step is carried out by:
(i) mixing fibroblast cells with a hydrogel to form a composition comprising fibroblast cells;
(ii) dispensing the composition comprising fibroblast cells onto the bodily surface;
(iii) crosslinking the composition comprising fibroblast cells to form a gel layer;
and
(iv) dispensing a composition comprising keratinocytes over the gel layer.

2. The method of claim 1 , wherein the dispensing step (ii) is carried out not more than five minutes after said mixing step (i).

3. The method of claim 1 , wherein said hydrogel comprises fibrinogen.

4. The method of claim 1 , wherein said hydrogel comprises collagen.

5. The method of claim 1 , wherein the composition comprising keratinocytes comprises a hydrogel.

6. The method of claim 5 , wherein the keratinocytes is mixed in the hydrogel not more than five minutes prior to the dispensing step (iv).

7. The method of claim 1 , wherein said fibroblast cells are delivered at a concentration of about 50,000 to 500,000 cells per cm 2 .

8. The method of claim 1 , wherein said keratinocytes are delivered at a concentration of about 50,000 to 500,000 cells per cm 2 .

9. The method of claim 1 , wherein said fibroblast cells and said keratinocytes are autologous with respect to said patient.

10. The method of claim 9 , wherein said fibroblast cells are produced by obtaining cells from said patient and culturing said cells to form a cell culture.

11. The method of claim 9 , wherein said keratinocytes are produced by obtaining cells from said patient and culturing said cells to form a cell culture.

12. The method of claim 1 , wherein said optical detector is a hand-held scanner.

13. The method of claim 1 , wherein the dispensing step (ii) or the dispensing step (iv) is carried out in a time of from 5 to 30 minutes.

14. The method of claim 1 , wherein the dispensing step (ii) or the dispensing step (iv) is carried out by depositing drops of said composition along said path, wherein said drops have a volume of from 1 to 500 microliters.

15. The method of claim 14 , wherein said drops have a volume of from 50 to 250 microliters.

16. The method of claim 1 , wherein said composition comprises viable cells and wherein said dispensing step comprises forming a tissue.

17. The method of claim 16 , wherein said tissue is skin tissue.

18. The method of claim 1 , wherein said optical detector is a scanner.

19. The method of claim 1 , wherein the Z-axis layers correspond to one or more tissue layers.

20. The method of claim 1 , wherein said depth detector comprises a range camera that collects three-dimensional scene information from a continuously-projected light.

21. The method of claim 20 , wherein said continuously-projected light is an infrared structured light.

22. The method of claim 1 , wherein the three-dimensional coordinates are updated in real time during said dispensing.

Assignees

• Univ Wake Forest Health Sciences

Inventors

• Yoo James J
• Atala Anthony
• Binder Kyle W
• Albanna Mohammad Z
• Zhao Weixin
• Dice Dennis
• Xu Tao

Classifications

• A61M35/20

  Non-portable devices, e.g. spraying booths · CPC title

• A61B90/50

  Supports for surgical instruments, e.g. articulated arms · CPC title

• A61B2034/105

  Modelling of the patient, e.g. for ligaments or bones · CPC title

• A61L27/225

  Fibrin; Fibrinogen · CPC title

• A61B5/0064 Primary

  Body surface scanning · CPC title