Specific detection and quantification of cardiolipin and isolated mitochondria by positively charged AIE fluorogens and method of manufacturing thereof

Patent metadata

Field	Value
Publication number	US-10113968-B2
Application number	US-201415100707-A
Country	US
Kind code	B2
Filing date	Dec 3, 2014
Priority date	Dec 3, 2013
Publication date	Oct 30, 2018
Grant date	Oct 30, 2018

Abstract

The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

First claim

We claim:

1. A one-step method of detecting and quantifying cardiolipin in a sample using a positively charged aggregation induced emission (AIE) luminogen comprising:
introducing the AIE luminogen to a solution containing the sample; and
measuring fluorescence intensity of the solution, wherein
the AIE luminogen binds to cardiolipin in vesicles and comprises a structure having a formula of

2. The method of claim 1 , wherein the AIE luminogen binds negatively charged cardiolipin solely by electrostatic interaction.

3. A method of quantifying isolated mitochondria using a positively charged AIE luminogen comprising:
staining a sample containing isolated mitochondria with the AIE luminogen; and
measuring the fluorescence intensity wherein the AIE luminogen comprises a structure having a formula of

4. The method of claim 3 , wherein the quantified isolated mitochondria is from Saccharomyces cerevisiae strain YPH 500 of yeast.

5. A method of quantifying isolated mitochondria using a positively charged AIE luminogen comprising:
introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria; and
identifying the stained isolated mitochondria under microscope wherein the AIE luminogen comprises a structure having a formula of

6. The method of claim 5 , wherein the quantified isolated mitochondria is from Saccharomyces cerevisiae strain YPH 500 of yeast.

Assignees

• Univ Hong Kong Sci & Tech

Inventors

• Tang Benzhong
• Hong Yuning
• Leung Wai Tung

Classifications

• G01N33/483

  Physical analysis of biological material · CPC title

• G01N21/6458 Primary

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title