Imaging apparatus and imaging method

Patent metadata

Field	Value
Publication number	US-10113859-B2
Application number	US-201615264019-A
Country	US
Kind code	B2
Filing date	Sep 13, 2016
Priority date	Dec 11, 2015
Publication date	Oct 30, 2018
Grant date	Oct 30, 2018

Abstract

An OCT imaging technique capable of suppressing image noise due to reflection on a wall surface of a carrier for carrying an imaging object by a simple configuration is provided. A light regulating member 28 having a transmission pattern where the high transmission parts P1 and the low transmission parts P2 are alternately arranged is placed on a side opposite to the spheroid Sp (imaging object) across the objective lens 27. The transmission pattern is rotationally symmetric with respect to an optical axis AX of the objective lens 27 and a point located at a position point-symmetric with an arbitrary point in the high transmission part with respect to a point where the optical axis of the objective lens intersects with the light regulating surface is included in the low transmission part.

First claim

What is claimed is:

1. An imaging apparatus for tomographically imaging an imaging object carried in a carrier having an optically transparent wall surface, the imaging apparatus comprising:
a light source which emits low-coherence light to be split into illumination light and reference light, the illumination light being incident on the imaging object via an object optical system and reflected by the imaging object, reflected light from the imaging object passing through the object optical system;
an imaging unit which detects interference light generated by an interference of the reflected light and the reference light, and outputs an interference signal corresponding to the interference light; and
a signal processor which obtains a reflected light intensity distribution of the imaging object along an incident direction of the illumination light based on the interference signal, wherein
the object optical system includes an objective lens and a light regulator,
the objective lens is arranged to face the wall surface, converges the illumination light to the imaging object via the wall surface and collects the reflected light from the imaging object emitted via the wall surface,
the light regulator is arranged on an optical path of the illumination light on a side opposite to the imaging object across the objective lens and has a light regulating surface formed with a transmission pattern in which a high transmission part for transmitting the illumination light at a relatively high transmittance and a low transmission part having a lower transmittance to the illumination light than the high transmission part are regularly arranged,
the transmission pattern is rotationally symmetric with respect to an optical axis of the objective lens and a point located at a position point-symmetric with an arbitrary point in the high transmission part with respect to a point where the optical axis of the objective lens intersects with the light regulating surface is included in the low transmission part, and
the light regulating surface is arranged at a focus position of the objective lens.

2. The imaging apparatus according to claim 1 , wherein the transmission pattern is a pattern in which the high transmission part and the low transmission part are alternately allocated to a plurality of sector-shaped areas formed by dividing a circle centered on one point by three or more odd number of straight lines intersecting at the one point and arranged at equal angular intervals.

3. The imaging apparatus according to claim 1 , wherein a point located at a position point-symmetric with an arbitrary point in the low transmission part with respect to a point through which the optical axis of the objective lens passes is included in the high transmission part on the light regulating surface.

4. The imaging apparatus according to claim 1 , wherein an area of the high transmission part and an area of the low transmission part are equal in an area effectively functioning as the light regulating surface.

5. The imaging apparatus according to claim 1 , wherein the low transmission part has a light absorbing or scattering property.

6. The imaging apparatus according to claim 1 , wherein the light regulator includes a flat plate which has a light shielding property and on which an opening as the high transmission part is partially provided.

7. The imaging apparatus according to claim 1 , wherein the light regulator includes a flat plate which has optical transparency and on which light shielding surface layer as the low transmission part is partially formed.

8. The imaging apparatus according to claim 1 , wherein the objective lens is arranged such that the optical axis is perpendicular to the wall surface.

9. The imaging apparatus according to claim 1 , wherein
the light source emits light having a plurality of wavelength components and
the imaging unit spectrally diffracts the interference light and outputs a light intensity of each wavelength component as the interference signal.

10. The imaging apparatus according to claim 1 , wherein the imaging unit includes:
an optical device which has a function of dividing light from the light source into a plurality of branch lights and a function of mixing the reflected light and the reference light, one of the branch lights being the illumination light;
a reflector which reflects another branch light from the optical device toward the optical device, the reflected another branch light serving as the reference light, the reflector being positioned so that an optical path length of the reference light is approximately equivalent to an optical path length of the reflected light from the imaging object; and
a photo-detector which receives mixed light of the reflected light and the reference light emitted from the optical device and outputs the interference signal.

11. An imaging method for tomographically imaging an imaging object carried in a carrier having an optically transparent wall surface, the imaging method comprising:
dividing low-coherence light emitted from a light source into illumination light and reference light, the illumination light being incident on the imaging object via an object optical system and reflected by the imaging object, reflected light from the imaging object passing through the object optical system;
detecting interference light generated by an interference of the reflected light and the reference light; and
obtaining a reflected light intensity distribution of the imaging object along an incident direction of the illumination light based on the detected interference light, wherein
the object optical system includes an objective lens and a light regulator,
the objective lens is arranged to face the wall surface, converges the illumination light to the imaging object via the wall surface and collects the reflected light from the imaging object emitted via the wall surface,
the light regulator is arranged on an optical path of the illumination light on a side opposite to the imaging object across the objective lens and has a light regulating surface formed with a transmission pattern in which a high transmission part for transmitting the illumination light at a relatively high transmittance and a low transmission part having a lower transmittance to the illumination light than the high transmission part are regularly arranged,
the transmission pattern is rotationally symmetric with respect to an optical axis of the objective lens and a point located at a position point-symmetric with an arbitrary point in the high transmission part with respect to a point where the optical axis of the objective lens intersects with the light regulating surface is included in the low transmission part, and
the light regulating surface is arranged at a focus position of the objective lens.

Assignees

• Screen Holdings Co Ltd

Inventors

• Ueyama Kenji
• Fukue Kumiko

Classifications

• G01N33/4833

  of solid biological material, e.g. tissue samples, cell cultures (tissue in vivo A61B5/00; cell suspensions G01N33/48735) · CPC title

• G01B9/02091 Primary

  Tomographic interferometers, e.g. based on optical coherence · CPC title

• G01B9/02001

  characterised by controlling or generating intrinsic radiation properties · CPC title

• G01B9/02044

  Imaging in the frequency domain, e.g. by using a spectrometer · CPC title

• G01B9/02087

  Combining two or more images of the same region · CPC title