Microorganisms having enhanced L-amino acids productivity and process for producing L-amino acids using the same

Patent metadata

Field	Value
Publication number	US-10113191-B2
Application number	US-201515112855-A
Country	US
Kind code	B2
Filing date	Mar 17, 2015
Priority date	Mar 21, 2014
Publication date	Oct 30, 2018
Grant date	Oct 30, 2018

Abstract

Disclosed are a recombinant microorganism having enhanced L-amino acid productivity, wherein the recombinant microorganism is transformed to have removed or decreased activity of at least one of adenosine deaminase and AMP nucleosidase, and a method of producing an L-amino acid using the recombinant microorganism. The use of the recombinant microorganism may enable the production of the L-amino acid in a highly efficient manner.

First claim

The invention claimed is:

1. A recombinant microorganism, wherein activity of at least one of adenosine deaminase comprising the amino acid sequence of SEQ ID NO: 14 and AMP nucleosidase comprising the amino acid sequence of SEQ ID NO: 16 is removed or decreased compared to that of a mother strain, wherein the recombinant microorganism belongs to the genus Escherichia and has enhanced producibility of an L-amino acid compared to that of the mother strain, wherein the L-amino acid is L-threonine or L-tryptophan, and wherein the recombinant microorganism does not contain chloramphenicol marker gene.

2. The recombinant microorganism of claim 1 , wherein the recombinant microorganism is Escherichia coli.

3. The recombinant microorganism of claim 1 , wherein activity of adenosine deaminase comprising the amino acid sequence of SEQ ID NO: 14 and AMP nucleosidase comprising the amino acid sequence of SEQ ID NO: 16 are removed or decreased compared to that of the mother strain.

4. The recombinant microorganism of claim 1 , wherein at least one of a gene encoding adenosine deaminase comprising the amino acid sequence of SEQ ID NO: 14 and a gene encoding AMP nucleosidase comprising the amino acid sequence of SEQ ID NO: 16 is deleted or modified to be disrupted.

5. The recombinant microorganism of claim 4 , wherein at least one of the genes is modified such that a part or all of the gene or regulatory factors on promoter or terminator regions of the genes are mutated, substituted, deleted, or inserted.

6. A method of producing L-amino acid, the method comprising:
culturing a recombinant microorganism; and
collecting an L-amino acid from the culture, wherein the L-amino acid is L-threonine or L-tryptophan,
wherein the recombinant microorganism has an activity of at least one of adenosine deaminase comprising the amino acid sequence of SEQ ID NO:14 and AMP nucleosidase comprising the amino acid sequence of SEQ ID NO:16 removed or decreased compared to that of a mother strain, does not contain chloramphenical marker gene, and has enhanced producibility of L-threonine or L-tryptophan compared to that of the mother strain.

7. The method of claim 6 , wherein the recombinant microorganism is an Escherichia.

8. The method of claim 6 , wherein the recombinant microorganism is an Escherichia coli.

9. The method of claim 6 , wherein activity of adenosine deaminase comprising the amino acid sequence of SEQ ID NO: 14 and AMP nucleosidase comprising the amino acid sequence of SEQ ID NO: 16 in the recombinant microorganism are removed or decreased compared to that of the mother strain.

10. The method of claim 6 , wherein at least one of a gene encoding adenosine deaminase comprising the amino acid sequence of SEQ ID NO: 14 and a gene encoding AMP nucleosidase comprising the amino acid sequence of SEQ ID NO: 16 in the recombinant microorganism is deleted or modified to be disrupted.

11. The method of claim 10 , wherein at least one of the genes is modified such that a part or all of the genes or regulatory factors on promoter or terminator regions of the genes are mutated, substituted, deleted, or inserted.

Assignees

• Cj Cheiljedang Corp

Inventors

• Koh Eun Sung
• Kwon Su Yon
• Lee Kwang Ho
• Lee Ji Sun
• Jang Juno
• Lee Keun Cheol
• Hong Hyeong Pyo

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12Y305/04004

  Adenosine deaminase (3.5.4.4) · CPC title

• C12N9/78

  acting on carbon to nitrogen bonds other than peptide bonds (3.5) · CPC title

• C12P13/227 Primary

  Tryptophan · CPC title