Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
Promoter exhibiting high expression activity in mortierella microorganisms
US10113173B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10113173-B2 |
| Application number | US-201715675916-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 14, 2017 |
| Priority date | Mar 27, 2013 |
| Publication date | Oct 30, 2018 |
| Grant date | Oct 30, 2018 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus Mortierella . The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 28, or a variant thereof.
First claim
Opening claim text (preview).
What is claimed is: 1. A vector comprising a polynucleotide selected from: (a) a polynucleotide which contains any one nucleotide sequence selected from SEQ ID NO: 10, SEQ ID NO:11 and SEQ ID NO: 12; or (b) a polynucleotide which has a nucleotide sequence sharing an identity of 90% or more with any one nucleotide sequence selected from SEQ ID NO: 10, SEQ ID NO:11 and SEQ ID NO: 12 and which shows promoter activity in cells of microorganisms belonging to the genus Mortierella. 2. The vector according to claim 1 , wherein the promoter activity is confirmed as β-D-glucuronidase (GUS) protein activity of at least 500 nmol/(mg·min) upon expression of GUS reporter gene in cells of microorganisms belonging to the genus Mortierella. 3. The vector according to claim 1 , which comprises any one nucleotide sequence selected from SEQ ID NO: 10, SEQ ID NO:11 and SEQ ID NO: 12. 4. The vector according to claim 1 , which is DNA. 5. A microbial transformant transformed with the vector according to claim 1 . 6. The transformant according to claim 5 , wherein the transformant is a lipid-producing fungus. 7. The transformant according to claim 6 , wherein the lipid-producing fungus is Mortierella alpina.
Assignees
Inventors
Classifications
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10113173B2 cover?
- The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus Mortierella . The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1 to 28, or a variant thereof.
- Who is the assignee on this patent?
- Suntory Holdings Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12N15/80. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 30 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).