Cell culture bag and method for manufacturing cell culture bag

Patent metadata

Field	Value
Publication number	US-10113143-B2
Application number	US-201514709553-A
Country	US
Kind code	B2
Filing date	May 12, 2015
Priority date	May 29, 2014
Publication date	Oct 30, 2018
Grant date	Oct 30, 2018

Abstract

A cell culture bag is provided. The cell culture bag includes: an external film of the cell culture bag; at least one sensor chip arranged on a culture fluid side of the external film, the at least one sensor chip including a layer reacting to an object to be detected; and a permeable film that covers a culture fluid side of the sensor chip, the permeable film permeating the object to be detected.

First claim

What is claimed is:

1. A cell culture bag comprising:
an external film;
at least one sensor chip arranged on an inner surface of the external film, the at least one sensor chip including a layer reacting to an object to be detected, the inner surface being on a culture fluid side of the external film; and
a permeable film that covers a culture fluid side of the sensor chip, the permeable film permeating the object to be detected,
wherein the permeable film covers the entire inner surface of the external film,
wherein the at least one sensor chip comprises a plurality of sensor chips arranged into a plurality of groups,
wherein a group of sensor chips having the largest size is arranged at the center of the plurality of groups, the remaining groups of sensor chips having a smaller size being arranged around the group of sensor chips having the largest size, and
wherein the group of sensor chips having the largest size is configured to generate a stronger signal than the remaining groups of sensor chips having a smaller size.

2. The cell culture bag according to claim 1 , wherein an arrangement of the group of sensor chips with largest size and the remaining groups of sensor chips having a smaller size aids in positioning an optical system with the group of sensor chips with the largest size, the optical system being located outside of the cell culture bag.

3. A method for manufacturing a cell culture bag comprising:
attaching a plurality of sensor chips including a layer reacting to an object to be detected to an inner surface of a roll material that becomes an external film, the inner surface being on a culture fluid side of the external film, the plurality of sensor chips being arranged into a plurality of groups;
stacking a roll material that becomes a permeable film to the roll material that becomes the external film to cover the plurality of sensor chips with the roll material that becomes the permeable film, the roll material that becomes the permeable film permeating the object to be detected, wherein the permeable film covers the entire inner surface of the external film of the cell culture bag; and
performing thermocompression bonding on both the roll materials,
wherein the plurality of sensor chips become embedded into the roll material that becomes the external film, by the performing thermocompression bonding on both the roll materials,
wherein a group of sensor chips having the largest size is arranged at the center of the plurality of groups, the remaining groups of sensor chips having a smaller size being arranged around the group of sensor chips having the largest size,
wherein the group of sensor chips having the largest size is configured to generate a stronger signal than the remaining groups of sensor chips having the smaller size, and
wherein an arrangement of the group of sensor chips with largest size and the remaining groups of sensor chips having the smaller size aids in positioning an optical system with the group of sensor chips with the largest size, the optical system being located outside of the cell culture bag.

4. A method for manufacturing a cell culture bag comprising:
attaching a plurality of sensor chips including a layer reacting to an object to be detected to an inner surface of a roll material that becomes an external film, the inner surface being on a culture fluid side of the external film, the plurality of sensor chips being arranged into a plurality of groups;
stacking a roll material that becomes a permeable film to the roll material that becomes the external film to cover the plurality of sensor chips with the roll material that becomes the permeable film, the roll material that becomes the permeable film permeating the object to be detected, wherein the permeable film covers the entire inner surface of the external film of the cell culture bag; and
performing thermocompression bonding on both the roll materials,
wherein the plurality of sensor chips become embedded into the roll material that becomes the external film, by the performing thermocompression bonding on both the roll materials,
wherein a group of sensor chips having the largest size is arranged at the center of the plurality of groups, the remaining groups of sensor chips having a smaller size being arranged around the group of sensor chips having the largest size, and
wherein the group of sensor chips having the largest size is configured to generate a stronger signal than the remaining groups of sensor chips having the smaller size.

Assignees

• Yokogawa Electric Corp

Inventors

• Iino Toshio
• Hioki Tango

Classifications

• C12M41/00

  Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N) · CPC title

• C12M23/14 Primary

  Bags · CPC title

• C12M29/04

  Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title

• C12M41/34

  of gas · CPC title

• C12M41/32

  of substances in solution · CPC title