Protein purification in the presence of nonionic organic polymers and electropositive surfaces

Patent metadata

Field	Value
Publication number	US-10112971-B2
Application number	US-201414769101-A
Country	US
Kind code	B2
Filing date	Feb 26, 2014
Priority date	Feb 26, 2013
Publication date	Oct 30, 2018
Grant date	Oct 30, 2018

Abstract

A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.

First claim

What is claimed is:

1. A method of purifying an antibody from a preparation comprising:
(a) providing the preparation comprising an antibody;
(b) adding allantoin to the preparation to a supersaturating concentration, wherein after adding the allantoin, the preparation is supersaturated with allantoin;
(c) removing solids from the preparation;
(d) contacting the preparation with polyethylene glycol (PEG) having a molecular weight of 2,000 to 12,000 Daltons and a salt, wherein (i) a concentration of the PEG is sufficient to precipitate the antibody or cause its accretion on a first surface, or maintain it in a precipitated state or accreted on the first surface, and (ii) the salt concentration is sufficient to produce a conductivity between 50 mS/cm and 150 mS/cm; and
(e) contacting the preparation with at least one electropositive surface whereby the antibody does not adsorb to the at least one electropositive surface, wherein the contacting does not prevent adsorption of acidic contaminants to the at least one electropositive surface.

2. The method of claim 1 , further comprising (f) contacting the preparation with at least one nonionic surface.

3. The method of claim 1 , wherein the salt concentration of step (d) is the same as a concentration of the salt in the preparation of step (a).

4. The method of claim 1 , wherein the at least one electropositive surface is in the form of a porous membrane and the method further comprises retaining the precipitated antibody on the porous membrane while soluble contaminants are eliminated by passage there through.

5. The method of claim 1 , further comprising retaining the precipitated antibody on a microporous membrane that is substantially inert while soluble contaminants are eliminated by passage there through, independently from the salt concentration.

6. The method of claim 1 , further comprising retaining the precipitated antibody on a microporous membrane that is electropositive while soluble contaminants pass there through.

7. The method of claim 1 , wherein the method is conducted in a single integrated apparatus.

8. The method of claim 1 , wherein the polymer size of the nonionic organic polymer is in a range of 3,000 Daltons to 10,000 Daltons.

9. The method of claim 1 , wherein the salt is selected from the group consisting of sodium chloride, potassium chloride, sodium acetate, potassium acetate, sodium thiocyanate, potassium thiocyanate, guanidinium hydrochloride, and combinations thereof.

10. The method of claim 9 , wherein the salt comprises sodium chloride at a concentration from 0.5 M to 1.5 M.

11. The method of claim 1 , wherein the at least one electropositive surface comprises a membrane with pores of an average diameter from 100 nm to 2 microns.

12. The method of claim 1 , wherein the at least one electropositive surface comprises a plurality of particles.

13. The method of claim 1 , wherein the at least one electropositive surface is part of a chromatography device selected from the group comprising, a monolith-based chromatography device, a membrane-based chromatography device, a chromatography device comprising a plurality of particles, a macroreticulate skeleton supporting a hydrogel-based device, and combinations thereof.

14. The method of claim 1 , wherein a pH during step (a) is in a range from 6 to 9.

15. The method of claim 1 , wherein a pH during step (b) is in a range from 5 to 9.

16. The method of claim 1 , wherein the first surface or the at least one electropositive surface comprises one selected from the group consisting of a membrane, a monolith, and a plurality of particles, wherein the plurality particles are optionally magnetic.

17. The method of claim 1 , wherein the preparation comprises one selected from the group consisting of a cell culture medium, an extract from cultured organisms, and a bodily fluid.

Assignees

• Agency Science Tech & Res

Inventors

• Gagnon Peter Stanley

Classifications

• C07K1/30 Primary

  by precipitation · CPC title

• B01D15/3847

  Multimodal interactions · CPC title

• B01D15/363

  Anion-exchange · CPC title

• B01D15/34

  Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation · CPC title

• B01D15/305

  Hydrophilic interaction chromatography [HILIC] · CPC title