Tissue processing reagent

Patent metadata

Field	Value
Publication number	US-10107727-B2
Application number	US-201615371588-A
Country	US
Kind code	B2
Filing date	Dec 7, 2016
Priority date	Dec 30, 2015
Publication date	Oct 23, 2018
Grant date	Oct 23, 2018

Abstract

A method including placing a biological sample taken from a body into a chamber; adding a composition including an acetal solvent to the chamber; and fixating the biological sample. A method including placing a tissue from a body into a chamber; and contacting the tissue with a composition including an acetal solvent as a fixating process.

First claim

What is claimed is:

1. A method comprising:
placing a biological sample taken from a body into a chamber;
adding a composition comprising an acetal solvent to the chamber;
fixating the biological sample; and
after fixating the biological sample, containing the biological sample with a paraffin.

2. The method of claim 1 , wherein the acetal solvent comprises a dioxolane.

3. The method of claim 1 , wherein the acetal solvent comprises 1,3-dioxolane.

4. The method of claim 3 , wherein the composition comprises a mixture comprising the 1,3-dioxolane, a catalyst and water.

5. The method of claim 4 , wherein the catalyst is an acid.

6. The method of claim 4 , wherein the catalyst is an acid based catalyst.

7. The method of claim 4 , wherein the catalyst is a Lewis acid.

8. The method of claim 1 , wherein after fixating the biological sample, the method comprises dehydrating and clearing the biological sample wherein at least a part of at least one of dehydrating and clearing comprises treating the biological sample with a composition comprising an acetal solvent.

9. The method of claim 8 , wherein treating the biological sample with a composition comprising an acetal solvent comprises clearing the biological sample and, prior to clearing, the method comprises dehydrating the fixated biological sample.

10. The method of claim 9 , wherein dehydrating comprises treating the fixated biological sample with a dehydrating composition comprising an alcohol.

11. The method of claim 9 , wherein the dehydrating composition comprises at least one of acetone and a glycol.

12. The method of claim 1 , wherein after fixating the biological sample, the method comprises dehydrating, clearing the biological sample and infiltrating the biological sample, and infiltrating the biological sample comprises treating the biological sample with a composition comprising an acetal solvent.

13. The method of claim 12 , wherein the composition further comprises paraffin.

14. A method comprising:
placing a tissue taken from a body into a chamber;
contacting the tissue with a composition comprising an acetal solvent as a fixating process; and
after the fixating process, infiltrating the tissue with an embedding agent in an infiltration process.

15. The method of claim 14 , wherein the acetal solvent comprises a dioxolane.

16. The method of claim 15 , wherein the dioxolane comprises 1,3-dioxolane.

17. The method of claim 15 , wherein the composition comprises a mixture of the dioxolane, water and a catalyst.

18. The method of claim 17 , wherein the catalyst is an acid.

19. The method of claim 14 , wherein after the fixating process, the method comprises a dehydrating process, a clearing process and then the infiltration process wherein at least one of the dehydrating process, the clearing process and the infiltration process comprises contacting the tissue with an acetal solvent.

20. The method of claim 19 , wherein the acetal solvent in the at least one of the dehydrating process, the clearing process and the infiltration process comprises 1,3-dioxolane.

Assignees

• Sakura Finetek Usa Inc

Inventors

• Shah Amit D

Classifications

• G01N2001/364

  using resins, epoxy · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• G01N2001/305

  Fixative compositions · CPC title

• G01N1/36 Primary

  Embedding or analogous mounting of samples · CPC title

• G01N1/28 Primary

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title