Cell culture medium and process for protein expression, said medium and process comprising a PAM inhibitor
US-9062337-B2 · Jun 23, 2015 · US
Cell culture medium and process for protein expression, said medium and process comprising a PAM inhibitor
US10106828B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10106828-B2 |
| Application number | US-201514721679-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 26, 2015 |
| Priority date | Nov 9, 2010 |
| Publication date | Oct 23, 2018 |
| Grant date | Oct 23, 2018 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention is related to a cell culture medium for the expression of a protein, which medium comprises a PAM inhibitor, or a physiological equivalent thereof, and to a cell culture process for the expression of a protein, in which process a PAM inhibitor, or a physiological equivalent thereof, is used (FIG. 1 ).
First claim
Opening claim text (preview).
What is claimed is: 1. A cell culture process for the heterologous expression of a protein comprising cultivating cells in the presence of a peptidylglycine alpha amidating monooxigenase (PAM) inhibitor, or a physiological equivalent thereof, wherein cultivating cells is carried out in suspension culture, wherein the PAM inhibitor is 4-phenyl-3-butenoic acid (PBA), or a physiological equivalent thereof. 2. The process according to claim 1 , wherein the expression of a heterologous protein takes place in a mammalian cell based expression system. 3. The process according claim 1 , wherein said protein is at least one protein selected from the group consisting of an antibody, or a fragment or derivative thereof, a fusion protein, and non-antibody proteins. 4. The process according to claim 2 , wherein said mammalian cell-based expression system is at least one selected from the group consisting of Baby hamster Kidney cell lines, Chinese hamster ovary cell lines, Murine myeloma cell lines, Mouse myeloma cell line, Human embryonic kidney cell lines, Human-retina-derived cell lines, and Amniocyte cell lines. 5. The process according to claim 1 , wherein said process takes place in at least one bioreactor or culture vessel selected from the group consisting of shake flasks, T-flasks, roller bottles, bags, bioreactors, and spinner flasks. 6. The process according to claim 1 , wherein the PAM inhibitor, or a physiological equivalent thereof, serves to affect the formation of amidated amino acid residues, particularly C-terminal proline amide residues. 7. The process according to claim 1 , wherein 4-phenyl-3-butenoic acid (PBA), or a physiological equivalent thereof, is used in a concentration of >0.01 μM and <3M.
Assignees
Inventors
Classifications
Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title
Enzymes · CPC title
Serum-free culture media · CPC title
- C12P21/00Primary
Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title
Oxidoreductases (EC 1.) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10106828B2 cover?
- The present invention is related to a cell culture medium for the expression of a protein, which medium comprises a PAM inhibitor, or a physiological equivalent thereof, and to a cell culture process for the expression of a protein, in which process a PAM inhibitor, or a physiological equivalent thereof, is used (FIG. 1 ).
- Who is the assignee on this patent?
- Sandoz Ag
- What technology area does this patent fall under?
- Primary CPC classification C12P21/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 23 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).