Polysaccharide fraction originating in persimmon leaf with immunostimulating activation and antitumor activation and method for manufacturing same

Patent metadata

Field	Value
Publication number	US-10105439-B2
Application number	US-201514871220-A
Country	US
Kind code	B2
Filing date	Sep 30, 2015
Priority date	Apr 2, 2013
Publication date	Oct 23, 2018
Grant date	Oct 23, 2018

Abstract

A persimmon leaf-derived polysaccharide fraction consisting of 60-80 wt % of neutral sugar and 18-39 wt % of uronic acid, and 0.5-10 wt % of 3-deoxy-D-manno-2-octulosonic acid (KDO) analogs, the wt % based on the total weight of the polysaccharide fraction.

First claim

What is claimed is:

1. A persimmon leaf-derived polysaccharide fraction consisting of 60-80 wt % of neutral sugar and 18-39 wt % of uronic acid, and 0.5-10 wt % of 3-deoxy-D-manno-2-octulosonic acid (KDO) analogs,
wherein the wt % is based on the total weight of the polysaccharide fraction,
wherein the persimmon leaf-derived polysaccharide fraction is prepared by a method comprising:
(a) treating a persimmon leaf powder with pectinase;
(b) collecting a fraction having a molecular weight in a range of 3 to 300 kDa from an enzymatically treated persimmon leaf powder; and
(c) collecting a fraction having a molecular weight in a range of 5 to 30 kDa from the collected fraction in step (b),

wherein the neutral sugar comprises 20-40 mole % of arabinose, 10-40 mole % of rhamnose, 10-40 mole % of galactose, 1-10 mole % of fucose, 1-10 mole % of glucose, and 0.4-26 mole % of Rhamnogalacturonan-II indicator polysaccharides,
wherein mole % is based on the total mole of the neutral sugar in the persimmon leaf-derived polysaccharide fraction, and
wherein the persimmon leaf-derived polysaccharide fraction has a molecular weight of 5-30 kDa.

2. The persimmon leaf-derived polysaccharide fraction of claim 1 , wherein the uronic acid consists of galacturonic acid and glucuronic acid.

3. The persimmon leaf-derived polysaccharide fraction of claim 1 , wherein KDO analogs consist of 3-deoxy-D-lyxo-2-heptulosaric acid (DHA) and KDO.

4. The persimmon leaf-derived polysaccharide fraction of claim 1 , wherein:
the Rhamnogalacturonan-II indicator polysaccharides consist of 0.1-8 mole % of 2-methylfucose, 0.1-8 mole % of 2-methylxylose, 0.1-5 mole % of apiose, and 0.1-5 mole % of aceric acid; and
mole % is based on the total mole of the neutral sugar in the persimmon leaf-derived polysaccharide fraction.

5. The persimmon leaf-derived polysaccharide fraction of claim 1 , wherein the persimmon leaf-derived polysaccharide fraction possesses an activity of enhancing immunity.

6. The persimmon leaf-derived polysaccharide fraction of claim 1 , wherein the persimmon leaf-derived polysaccharide fraction possesses an activity of inhibiting a metastasis of cancer.

7. A food composition for enhancing immunity comprising the persimmon leaf-derived polysaccharide fraction of claim 1 as an active ingredient.

8. A food composition for preventing or improving cancer comprising the persimmon leaf-derived polysaccharide fraction of claim 1 as an active ingredient.

9. An anti-cancer adjuvant preparation comprising the persimmon leaf-derived polysaccharide fraction of claim 1 as an active ingredient.

Assignees

• Korea Food Res Inst
• Kyonggi Univ Industry & Academy Cooperation Foundation

Inventors

• Hong Hee-Do
• Lee Young-Chul
• Cho Chang-Won
• Rhee Young-Kyoung
• Kim Young-Chan
• Sung Su Kyung
• Kim Hee Jung
• Shin Kwang-Soon
• Park Hye-Ryung
• Jo Sun Young

Classifications

• A61K2039/55583

  Polysaccharides · CPC title

• A23L33/105

  Plant extracts, their artificial duplicates or their derivatives · CPC title

• A23L33/22

  Comminuted fibrous parts of plants, e.g. bagasse or pulp · CPC title

• A61K39/39 Primary

  characterised by the immunostimulating additives, e.g. chemical adjuvants · CPC title

• A23L33/10

  using additives (addition of substantially indigestible substances A23L33/21) · CPC title