Solution and method for adhering suspension components to a substrate

Patent metadata

Field	Value
Publication number	US-10101248-B1
Application number	US-201715787185-A
Country	US
Kind code	B1
Filing date	Oct 18, 2017
Priority date	Dec 2, 2015
Publication date	Oct 16, 2018
Grant date	Oct 16, 2018

Abstract

This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.

First claim

We claim:

1. A solution consisting essentially of:
an alcohol having a final concentration of 60-70% by volume;
acetylsalicyclic acid having a final concentration of 100-1000 ug/mL; and
water,
wherein the final concentrations are the concentrations of the respective components when mixed with a sample.

2. The solution of claim 1 , wherein the alcohol is methanol.

3. The solution of claim 1 , further comprising a buffer.

4. The solution of claim 1 , further comprising a salt, wherein the solution is hypotonic relative to the sample.

Assignees

• Rarecyte Inc

Inventors

• Campton Daniel
• Nordberg Joshua
• Seubert Ronald

Classifications

• G01N2001/305

  Fixative compositions · CPC title

• G02B21/34

  Microscope slides, e.g. mounting specimens on microscope slides · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• A61K47/10

  Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers · CPC title

• A61K31/60

  Salicylic acid; Derivatives thereof · CPC title