Solution and method for adhering suspension components to a substrate

Patent metadata

Field	Value
Publication number	US-10101247-B2
Application number	US-201514956622-A
Country	US
Kind code	B2
Filing date	Dec 2, 2015
Priority date	Jul 19, 2013
Publication date	Oct 16, 2018
Grant date	Oct 16, 2018

Abstract

This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.

First claim

We claim:

1. A method comprising:
adding a solution to a sample to form a solution-sample mixture, the solution consisting essentially of
an alcohol having a final concentration of 60-90% by volume,
heparin having a final concentration of 10-1000 ug/mL,
acetylsalicylic acid having a final concentration of 100-1000 ug/mL, and water,
wherein the final concentrations are the concentrations of the respective components when in the solution-sample mixture.

2. The method of claim 1 , further comprising dispensing the sample-solution mixture onto a substrate.

3. The method of claim 2 , further comprising curing the sample-solution mixture on the substrate after the dispensing step.

4. The method of claim 3 , further comprising spreading the sample-solution mixture across the substrate after the dispensing step and before the curing step.

5. The method of claim 1 , wherein the alcohol is methanol.

Assignees

• Rarecyte Inc

Inventors

• Campton Daniel
• Nordberg Joshua
• Seubert Ronald
• Quarre Steve

Classifications

• G01N1/2813

  Producing thin layers of samples on a substrate, e.g. smearing, spinning-on (G01N1/30 takes precedence) · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title