Methods and compositions related to thioesterase enzymes
US-9587231-B2 · Mar 7, 2017 · US
Method for producing 3-hydroxypropionic acid and other products
US10100342B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10100342-B2 |
| Application number | US-201615167439-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 27, 2016 |
| Priority date | Sep 27, 2009 |
| Publication date | Oct 16, 2018 |
| Grant date | Oct 16, 2018 |
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- Title
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Abstract
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This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for producing a product having malonyl-CoA as a metabolic intermediate in a microbial production pathway of the product, the method comprising combining a carbon source and a microorganism cell culture to produce said product in a fermentation, wherein: a) said microorganism is genetically modified for reduced enzymatic activity of a thioesterase; b) said microorganism is genetically modified for overexpression of accA, accB, accC, accD, or any combination thereof; and an enzymatic activity of a fatty acid synthase pathway is reduced after the fermentation is initiated, wherein the enzymatic activity of the fatty acid synthase pathway is reduced by reducing the enzymatic activity of an enzyme selected from the group consisting of beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP reductase, beta-hydroxyacyl-ACP dehydratase, and enoyl-ACP reductase. 2. The method of claim 1 , wherein said microorganism additionally is genetically modified for reduced enzymatic activity of a phosphotransferase (PTS), or for increased enzymatic activity of a pyruvate dehydrogenase (Pdh), or both. 3. The method of claim 1 , wherein the beta-ketoacyl-ACP synthase is fabH, fahF, or fabB, and the enoyl-ACP reductase is fabI. 4. The method of claim 1 , wherein the enzymatic activity of the fatty acid synthase pathway is reduced by reducing the enzymatic activity of enoyl ACP reductase. 5. The method of claim 4 , wherein the enoyl-ACP reductase is fabI. 6. The method of claim 4 , wherein the enoyl-ACP reductase has temperature sensitive enzyme activity. 7. The method of claim 1 , wherein the enzymatic activity of the fatty acid synthase pathway is reduced by reducing the enzymatic activity of beta-ketoacyl-ACP synthase. 8. The method of claim 7 , wherein the beta-ketoacyl-ACP synthase has temperature sensitive enzyme activity. 9. The method of claim 1 , wherein overexpression of accA, accB, accC, accD, or any combination thereof occurs by introduction of a heterologous nucleic acid sequence coding for a polypeptide having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOS. 772, 774, 776, and 778.
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Classifications
acting on ester bonds (3.1) · CPC title
acting on the aldehyde or oxo group of donors (1.2) · CPC title
- C12P11/00Primary
Preparation of sulfur-containing organic compounds · CPC title
Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1) · CPC title
Transferases transferring phosphorus-containing groups (2.7) · CPC title
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Frequently asked questions
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- What does patent US10100342B2 cover?
- This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
- Who is the assignee on this patent?
- Cargill Inc, Univ Colorado Regents
- What technology area does this patent fall under?
- Primary CPC classification C12P11/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 16 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).