Apparatus and method for preparing a biological sample for analysis

Patent metadata

Field	Value
Publication number	US-10099217-B2
Application number	US-201415100806-A
Country	US
Kind code	B2
Filing date	Dec 8, 2014
Priority date	Dec 12, 2013
Publication date	Oct 16, 2018
Grant date	Oct 16, 2018

Abstract

An apparatus for processing a biological sample is provided. The apparatus includes a body having a processing chamber, a first reservoir, a second reservoir, a filter, a first one-way valve, a second one-way valve, a first fluid pathway, and a second fluid pathway. The first fluid pathway and the second fluid pathway pass through the filter. A method of processing a sample using the apparatus is also provided. The method includes placing a sample comprising a liquid into the processing chamber; urging at least a portion of the liquid through the filter; after urging the at least a portion of the liquid through the filter, urging a portion of a back-flush liquid through the filter into the process chamber to form a first processed sample; and analyzing at least a portion of the first processed sample to detect an indication of a microorganism.

First claim

The invention claimed is:

1. A method of processing a sample, comprising:
placing a sample comprising a liquid into a processing chamber of an apparatus, the apparatus comprising:
a body comprising a first end and a second end opposite the first end, the body comprising:
a processing chamber comprising an opening proximate the first end;
a first reservoir disposed in selective fluid communication with the processing chamber via a first fluid pathway;
a second reservoir disposed in selective fluid communication with the processing chamber via a second fluid pathway;
a filter disposed in the body between at least a portion of the processing chamber and the second reservoir;
wherein the first fluid pathway and the second fluid pathway pass through the filter;
wherein the filter has a first surface oriented in the fluid pathways toward the processing chamber;
a first one-way valve disposed in the first fluid pathway between the filter and the first reservoir, the first one-way valve providing selective fluid communication between the processing chamber and the first reservoir; and
a second one-way valve disposed in the second fluid pathway between the filter and the second reservoir, the second one-way valve providing selective fluid communication between the processing chamber and the second reservoir;

urging at least a portion of the liquid out of the processing chamber through the filter;
after urging the at least a portion of the liquid through the filter, urging a first portion of a back-flush liquid from the first reservoir through the filter and into the process chamber to form a first processed sample; and
analyzing at least a portion of the sample to detect an indication of a microorganism, wherein analyzing at least a portion of the sample comprises analyzing at least a portion of the first processed sample.

2. The method of claim 1 , wherein urging at least a portion of the liquid through the filter comprises inserting a plunger into the processing chamber and using the plunger to generate a positive pressure in the processing chamber.

3. The method of claim 2 , wherein urging a first portion of the back-flush liquid through the filter from the second side comprises using the plunger to generate a negative pressure in the processing chamber.

4. The method of claim 1 , wherein placing a sample comprising a liquid into the processing chamber comprises placing a first predetermined volume of liquid into the processing chamber, wherein forming a first processed sample comprises forming a first processed sample have a second predetermined volume, wherein the second predetermined volume is less than or equal to the first predetermined volume.

5. The method of claim 4 , wherein forming a first processed sample having a second predetermined volume comprises using a visible indicium on the processing chamber or the first reservoir to define the second predetermined volume.

6. The method of claim 1 , further comprising:
after forming the first processed sample, urging at least a portion of the first processed sample out of the processing chamber through the filter;
urging a second portion of back-flush liquid from the first reservoir through the filter and into the process chamber to form a second processed sample;
wherein analyzing at least a portion of the sample to detect an indication of a microorganism comprises analyzing at least a portion of the second processed sample.

7. The method of claim 6 , wherein placing a sample comprising a liquid into the processing chamber comprises placing a first predetermined volume of liquid into the processing chamber, wherein forming a second processed sample comprises forming a second processed sample having a third predetermined volume, wherein the third predetermined volume is smaller than the first predetermined volume.

8. The method of claim 7 , wherein forming a second processed sample having a third predetermined volume comprises using a visible indicium on the processing chamber or the first reservoir to define the third predetermined volume.

9. The method of claim 1 , wherein analyzing at least a portion of the first processed sample or analyzing at least a portion of the second processed sample comprises analyzing the portion of the first processed sample or second processed sample to detect an antigen, a nucleic acid, an enzyme activity, or a viable microorganism.

10. The method of claim 1 , further comprising:
after urging at least a portion of the liquid through the filter, adjusting the temperature of the processing chamber to about 95-100° C.

11. The method of claim 10 , wherein adjusting the temperature of the processing chamber comprises placing the apparatus in a heated device.

Assignees

• 3M Innovative Properties Co

Inventors

• Kirschhoffer Jon A
• Sitton Gregory W
• Tilstra Andrew H

Classifications

• B01L3/502 Primary

  with fluid transport, e.g. in multi-compartment structures · CPC title

• B01L2400/0611

  duck bill valves · CPC title

• B01L2200/0689

  Sealing · CPC title

• B01L2400/0478

  pistons · CPC title

• B01L2300/0681

  Filter · CPC title