Cancer antigen-specific T-cell receptor gene, peptide encoded by the gene, and use of them

Patent metadata

Field	Value
Publication number	US-10093977-B2
Application number	US-52970108-A
Country	US
Kind code	B2
Filing date	Feb 28, 2008
Priority date	Mar 5, 2007
Publication date	Oct 9, 2018
Grant date	Oct 9, 2018

Abstract

Disclosed are: a nucleotide sequence and an amino acid sequence for CDR3 region of T-cell receptor (TCR) gene of WT1-specific cytotoxic T-cell (CTL) for WT1 protein; a method for the detection or treatment of cancer using the nucleotide sequence or the amino acid sequence; and a chip, a primer set, a kit, an apparatus and the like for use in the detection of cancer, each of which comprises the nucleotide sequence or the amino acid sequence.

First claim

The invention claimed is:

1. A synthesized cDNA, comprising:
(1) a nucleotide sequence encoding the entire amino acid sequence of a complementarity determining region 3 (CDR3) of a vβ chain of a T-cell receptor (TCR) of a WT1-specific HLA-A*2402-positive cytotoxic T-cell (CTL), wherein the nucleotide sequence is selected from the group consisting of SEQ ID NOs: 1-200, or
(2) the nucleotide sequence that is complementary to the full length of the nucleotide sequence of (1),
wherein the cDNA further comprises a label attached to the cDNA, said label selected from fluorescent labels, radioactive labels, enzyme labels, and chromophore labels.

2. The cDNA according to claim 1 , wherein said cDNA consists of:
(1) the nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-200, or
(2) the nucleotide sequence that is complementary to the full length of the nucleotide sequence of (1): and
the label.

3. The cDNA according to claim 1 , wherein the CDR3 is identified by comparing with a human TCR gene database.

Assignees

• Sugiyama Haruo
• Int Inst Cancer Immunology Inc

Inventors

• Sugiyama Haruo

Classifications

• C12Q1/6886

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• C07K14/705 Primary

  Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title

• C12Q2600/106

  Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism · CPC title

• C12Q1/6881 Primary

  for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes · CPC title

• C07K14/7051 Primary

  T-cell receptor (TcR)-CD3 complex · CPC title