AXMI477, AXMI482, AXMI486 and AXMI525 toxin genes and methods for their use

Patent metadata

Field	Value
Publication number	US-10093946-B2
Application number	US-201415102785-A
Country	US
Kind code	B2
Filing date	Dec 8, 2014
Priority date	Dec 9, 2013
Publication date	Oct 9, 2018
Grant date	Oct 9, 2018

Abstract

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a Bacillus thuringiensis toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5-26, or the nucleotide sequence set forth in SEQ ID NO: 1-4, as well as variants and fragments thereof.

First claim

That which is claimed:

1. A construct comprising a heterologous promoter operably linked to a nucleotide sequence encoding an amino acid sequence having pesticidal activity against a lepidopteran pest, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO:5-10;
c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any one of SEQ ID NO:5-10.

2. The construct of claim 1 , wherein said nucleotide sequence is a synthetic sequence that has been designed for expression in a plant.

3. The construct of claim 1 , wherein said promoter is capable of directing expression of said nucleotide sequence in a plant cell.

4. A vector comprising the construct of claim 1 .

5. The vector of claim 4 , further comprising a nucleic acid molecule encoding a heterologous polypeptide.

6. A host cell that contains the construct of claim 1 .

7. The host cell of claim 6 that is a bacterial host cell.

8. The host cell of claim 6 that is a plant cell.

9. A transgenic plant comprising the host cell of claim 8 .

10. The transgenic plant of claim 9 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.

11. A transgenic seed comprising the construct of claim 1 .

12. A recombinant polypeptide with pesticidal activity against a lepidopteran pest, wherein said polypeptide comprises a heterologous leader or transit peptide and wherein said polypeptide is selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of any one of SEQ ID NO:5-10; and
b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any one of SEQ ID NO:5-10.

13. The polypeptide of claim 12 further comprising heterologous amino acid sequences.

14. A composition comprising the polypeptide of claim 12 .

15. The composition of claim 14 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.

16. The composition of claim 14 , wherein said composition is prepared by desiccation, lyophilization, homogenization, extraction, filtration, centrifugation, sedimentation, or concentration of a culture of bacterial cells.

17. The composition of claim 14 , comprising from about 1% to about 99% by weight of said polypeptide.

18. A method for controlling a lepidopteran pest population comprising contacting said population with a pesticidally-effective amount of the polypeptide of claim 12 .

19. A method for killing a lepidopteran pest, comprising contacting said pest with, or feeding to said pest, a pesticidally-effective amount of the polypeptide of claim 12 .

20. A method for producing a polypeptide with pesticidal activity against a lepidopteran pest, comprising culturing the host cell of claim 6 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

21. A plant or plant cell having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity against a lepidopteran pest, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO:5-10; and
c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any one of SEQ ID NO:5-10.

22. A method for protecting a plant from a lepidopteran pest, comprising expressing in a plant or cell thereof a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO:5-10; and
c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any one of SEQ ID NO:5-10.

23. The method of claim 22 , wherein said plant produces a pesticidal polypeptide having pesticidal activity against a lepidopteran pest.

24. A method for increasing yield in a plant comprising growing in a field a plant or a seed thereof having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity against a lepidopteran pest, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO:5-10; and
c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any one of SEQ ID NO:5-10;

wherein said field is infested with a lepidopteran pest against which said polypeptide has pesticidal activity and wherein said yield is increased relative to the yield of a plant that does not express a nucleotide sequence encoding any one of SEQ ID NO:5-10.

Assignees

• Athenix Corp
• Basf Se

Inventors

• Lehtinen Duane Alan
• Sampson Kimberly S
• Roberts Kira
• Dunn Ethan

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• A01N47/44

  Guanidine; Derivatives thereof · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• Y02A40/162

  Cross-Sectional Technologies · mapped topic