Tobacco inbred and hybrid plants and uses thereof

Patent metadata

Field	Value
Publication number	US-10091963-B2
Application number	US-201715420799-A
Country	US
Kind code	B2
Filing date	Jan 31, 2017
Priority date	Mar 5, 2013
Publication date	Oct 9, 2018
Grant date	Oct 9, 2018

Abstract

The present disclosure provides tobacco inbred plants TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, and CMS TN86 SRC, and hybrids KY14×L8 SRC, NC7 SRC, and NCBH129 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

First claim

What is claimed is:

1. A method of producing an F1 progeny tobacco seed comprising:
a. crossing a first tobacco plant with a second tobacco plant, wherein said first tobacco plant is a tobacco plant produced by growing the seed of a tobacco cultivar elected from the group consisting of TN90 SRC, CMS TN90 SRC, KY14SRC, CMS KY14 SRC, L8 SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, CMS TN86 SRC, KY14×L8 SRC, NC7 SRC, and NCBH129 SRC; wherein a representative sample seed of said TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86SRC, CMS TN86 SRC, KY14×L8 SRC, NC7 SRC, and NCBH129 SRC has been deposited at the ATCC with the following ATCC Accession Nos.: ATCC Accession No. PTA-13567 for TN90 SRC, ATCC Accession No. PTA-13573 for CMS TN90 SRC, ATCC Accession No. PTA-120311 for KY14 SRC, ATCC Accession No. PTA-13571 for CMS KY14 SRC, ATCC Accession No. PTA-13572 for L8 SRC, ATCC Accession No. PTA-13569 for hybrid cultivar KY14×L8 SRC, ATCC Accession No. PTA-120312 for NC775 SRC, ATCC Accession No. PTA-13563 for CMS NC775 SRC, ATCC Accession No. PTA-13566 for NC645 SRC, ATCC Accession No. PTA-13564 for hybrid cultivar NC7 SRC, ATCC Accession No. PTA-120313 for NC638 SRC, ATCC Accession No. PTA-13568 for CMS NC638 SRC, ATCC Accession No. PTA-13565 for TN86 SRC, ATCC Accession No. PTA-13570 for CMS TN86 SRC, and ATCC Accession No. PTA-13562 for hybrid cultivar NCBH129 SRC; and
b. harvesting the resultant tobacco seed.

2. The method of producing a tobacco seed of claim 1 , wherein said first tobacco plant is the male parent.

3. The method of producing a tobacco seed of claim 1 , wherein said first tobacco plant is the female parent.

4. The method of producing a tobacco seed of claim 1 , wherein at least one said tobacco plant is a cytoplasmic male sterile (CMS).

5. A container of F1 progeny seeds of claim 1 .

6. The container of F1 progeny seeds of claim 5 , wherein said container is a box, a bag, a packet, a pouch, a tape roll, a pail, a foil, or a tube.

7. The, container of F1 progeny seeds of claim 5 , wherein greater than about 50% of plants grown from said F1 progeny seeds have decreased nornicotine.

8. The container of F1 progeny seeds of claim 7 , wherein greater than 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% of plants grown from said F1progeny seeds have decreased nornicotine.

9. The container of F1 progeny seeds of claim 5 , wherein alkaloids obtained from tobacco plants grown from greater than about 50% of said F1progeny seeds have decreased nornicotine.

10. A harvested leaf of an F1 progeny plant, wherein said F1 progeny plant is of a tobacco cultivar selected from the group consisting of TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, CMS TN86 SRC, KY14×L8 SRC, NC7 SRC, and NCBH129 SRC; wherein a representative sample seed of said TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, CMS TN86 SRC, KY14×L8 SRC, NC7 SRC, and NCBH129 SRC has been deposited at the ATCC with the following ATCC Accession Nos.: ATCC Accession No. PTA-13567 for TN90 SRC, ATCC Accession No. PTA-13573 for CMS TN90 SRC, ATCC Accession No. PTA-120311 for KY14 SRC, ATCC Accession No. PTA-13571 for CMS KY14 SRC, ATCC Accession No. PTA-13572 for L8 SRC, ATCC Accession No. PTA-13569 for hybrid cultivar KY14×L8SRC, ATCC Accession No. PTA-120312 for NC775 SRC, ATCC Accession No. PTA-13563 for CMS NC775 SRC, ATCC Accession No. PTA-13566 for NC645SRC, ATCC Accession No. PTA-13564 for hybrid cultivar NC7 SRC, ATCC Accession No. PTA-120313 for NC638 SRC, ATCC Accession No. PTA-13568 for CMS NC638 SRC, ATCC Accession No. PTA-13565 for TN86 SRC, ATCC Accession No. PTA-13570 for CMS TN86 SRC, and ATCC Accession No. PTA-13562 for hybrid cultivar NCBH129 SRC,
wherein said harvested leaf has a reduced amount of nornicotine and/or N′-nitrosonornicotine (NNN) compared to a control leaf having a similar genetic background without any of the cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S alleles.

11. The harvested leaf of claim 10 , wherein said reduced amount of nornicotine and/or N-nitrosonornicotine (NNN) is reduced in a smoke stream produced from said leaf compared to a control leaf having a similar genetic background without any of the cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S alleles.

12. A tobacco product prepared from an F1 progeny tobacco plant, or part thereof,
wherein said F1 progeny plant is of a tobacco cultivar selected from the group consisting of TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8 SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, CMS TN86 SRC, KY14×L8 SRC, NC7 SRC, and NCBH129 SRC; wherein a representative sample seed of said TN90 SRC, CMS TN90 SRC, KY14 SRC, CMS KY14 SRC, L8SRC, NC775 SRC, CMS NC775 SRC, NC645 SRC, NC638 SRC, CMS NC638 SRC, TN86 SRC, CMS TN86 SRC, KY14×L8 SRC, NC7 SRC, and NCBH129 SRC has been deposited at the ATCC with the following ATCC Accession Nos.: ATCC Accession No. PTA-13567 for TN90 SRC, ATCC Accession No. PTA-13573 for CMS TN90 SRC, ATCC Accession No. PTA-120311 for KY14 SRC, ATCC Accession No. PTA-13571for CMS KY14 SRC, ATCC Accession No. PTA-13572 for L8 SRC, ATCC Accession No. PTA-13569 for hybrid cultivar KY14×L8 SRC, ATCC Accession No. PTA-120312for NC775 SRC, ATCC Accession No. PTA-13563 for CMS NC775 SRC, ATCC Accession No. PTA-13566 for NC645 SRC, ATCC Accession No. PTA-13564 for hybrid cultivar NC7 SRC, ATCC Accession No. PTA-120313 for NC638 SRC, ATCC Accession No. PTA-13568 for CMS NC638 SRC, ATCC Accession No. PTA-13565 for TN86 SRC, ATCC Accession No. PTA-13570 for CMS TN86 SRC, and ATCC Accession No. PTA-13562 for hybrid cultivar NCBH129 SRC.

13. The F1 progeny plant of claim 12 , wherein said F1 plant is male sterile.

14. The tobacco product of claim 12 , wherein said tobacco product has an amount of nornicotine of less than about 3 mg/g.

Assignees

• Univ North Carolina State

Inventors

• Lewis Ramsey S
• Dewey Ralph E

Classifications

• A24B15/10

  Chemical features of tobacco products or tobacco substitutes · CPC title

• A01H5/12 Primary

  Leaves · CPC title

• A01H6/823 Primary

  Nicotiana, e.g. tobacco · CPC title

• C12N15/8243

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C12N9/1007

  Methyltransferases (general) (2.1.1.) · CPC title