Assay for detection of human parvovirus nucleic acid

Patent metadata

Field	Value
Publication number	US-10087494-B2
Application number	US-201414569338-A
Country	US
Kind code	B2
Filing date	Dec 12, 2014
Priority date	Feb 26, 2009
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

First claim

I claim:

1. An amplification oligomer combination for amplifying human parvovirus genotypes 1, 2, and 3, the oligomer combination comprising:
a) at least one primer oligomer member comprising a target binding sequence that is SEQ ID NO:48 or SEQ ID NO:50;
b) a first promoter-based oligomer member comprising a sequence that is SEQ ID NO:73; and
c) a second promoter-based oligomer member comprising a sequence that is SEQ ID NO:78.

2. An amplification oligomer combination for amplifying human parvovirus genotypes 1, 2, and 3, the oligomer combination comprising at least one primer oligomer member and at least one promoter-based oligomer member, wherein the at least one primer oligomer member and the at least one promoter-based oligomer member hybridize to opposing strands of a human parvovirus genotype 1, 2 or 3 nucleic acid or amplicon thereof for transcription mediated amplification of the target nucleic acid or amplicon thereof between the hybridized primer and promoter-based oligomer members, wherein
A) the at least one promoter-based oligomer member is a first promoter-based oligomer member that is SEQ ID NO:76 and a second promoter-based oligomer member that is SEQ ID NO:81;
B) the at least one promoter-based oligomer member is selected from the group consisting of SEQ ID NOS:58, 63, 67, 68, 73, 78, 82, and combinations thereof;
C) the at least one primer oligomer member is SEQ ID NO:50 and the at least one promoter-based oligomer member is a first promoter-based oligomer member that is SEQ ID NO:73 and a second promoter-based oligomer member that is SEQ ID NO:78; or
D) the at least one primer oligomer member is SEQ ID NO:47 and the at least one promoter-based oligomer member is selected from the group consisting of SEQ ID NOs:73, 76, 78, 81 and combinations thereof.

3. The amplification oligomer combination of claim 1 , wherein the at least one primer oligomer member comprises the target-binding sequence that is SEQ ID NO:50.

4. The amplification oligomer combination of claim 1 , wherein the at least one primer oligomer member comprises the target-binding sequence that is SEQ ID NO:48.

5. The amplification oligomer combination of claim 1 , wherein the at least one primer oligomer member is SEQ ID NO:47.

6. The amplification oligomer combination of claim 1 , wherein the at least one primer member is selected from the group consisting of SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50 and combinations thereof.

7. A reaction mixture for performing a transcription mediated amplification reaction, wherein the reaction mixture comprises:
a) an amplification oligomer combination comprising:
i) at least one primer oligomer member comprising a target binding sequence that is SEQ ID NO:48 or SEQ ID NO:50;
ii) a first promoter-based oligomer member comprising a sequence that is SEQ ID NO:73; and
iii) a second promoter-based oligomer member comprising a sequence that is SEQ ID NO:78;
and

b) an enzyme comprising an activity selected from the group consisting of reverse transcriptase activity, RNA polymerase activity, and RNase H activity.

8. The reaction mixture of claim 7 , wherein the enzyme comprises reverse transcriptase activity and RNase H activity.

9. The amplification oligomer combination of claim 2 , wherein the at least one promoter-based oligomer member comprises a blocking moiety at its 3′ end.

Assignees

• Gen Probe Inc

Inventors

• Carrick James M

Classifications

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12Q1/701 Primary

  Specific hybridization probes · CPC title

• C12Q1/702 Primary

  for retroviruses · CPC title