Process and apparatus for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

Patent metadata

Field	Value
Publication number	US-10087475-B2
Application number	US-201515300278-A
Country	US
Kind code	B2
Filing date	Jan 29, 2015
Priority date	Apr 3, 2014
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

The disclosure relates to a process for the preparation of a sugar product from lignocellulosic material, comprising the following steps:
a) optionally, pretreatment of the lignocellulosic material; b) optionally, washing of the optionally pretreated lignocellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material in a hydrolysis reactor using an enzyme composition comprising at least two cellulase; and d) optionally, recovery of a sugar product; wherein during the enzymatic hydrolysis oxygen-containing gas is added to the lignocellulosic material in the hydrolysis reactor and wherein part of the oxygen-containing gas, added to the lignocellulosic material, is gas originating from the headspace of the reactor, preferably during part of the time of the enzymatic hydrolysis less oxygen is added to the lignocellulosic material compared to the other part of the time of the enzymatic hydrolysis, or preferably during part of the time of the enzymatic hydrolysis no oxygen is added to the lignocellulosic material.

First claim

The invention claimed is:

1. A process for the preparation of a sugar product from lignocellulosic material, comprising:
a) optionally, pretreatment of the lignocellulosic material;
b) optionally, washing of the optionally pretreated lignocellulosic material;
c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material in a hydrolysis reactor using an enzyme composition comprising at least two cellulases; and
d) optionally, recovery of a sugar product;
wherein during the enzymatic hydrolysis oxygen-containing gas is added to the lignocellulosic material in the hydrolysis reactor and wherein part of the oxygen-containing gas, added to the lignocellulosic material, is gas originating from the headspace of the reactor,
optionally during part of the time of the enzymatic hydrolysis less oxygen is added to the lignocellulosic material compared to the other part of the time of the enzymatic hydrolysis, or optionally during part of the time of the enzymatic hydrolysis no oxygen is added to the lignocellulosic material.

2. The process according to claim 1 , wherein oxygen is consumed in an amount corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material.

3. The process according to claim 1 , wherein the oxygen is added in the form of bubbles.

4. The process according to claim 1 , wherein the reactor for the enzymatic hydrolysis has a volume of 1 m 3 or more.

5. The process according to claim 1 , wherein the hydrolysis is conducted at a temperature of 45° C. or more.

6. The process according to claim 1 , wherein the enzyme composition:
is from a fungus;
is from a microorganism of the genus Rasamsonia
comprises a fungal enzyme; or
comprises a Rasamsonia enzyme.

7. The process according to claim 1 , wherein the dry matter content in the hydrolysis c) is 10 wt % or more.

8. The process according to claim 1 , in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.

9. The process according to claim 1 , in which oxygen is introduced as an oxygen-containing gas.

10. The process according to claim 9 , in which the oxygen-containing gas is air.

11. The process according to claim 1 , wherein during part of the time of the enzymatic hydrolysis less oxygen is added to the lignocellulosic material compared to the other part of the time of the enzymatic hydrolysis.

12. The process according to claim 11 , wherein the part of the time wherein less oxygen is added is 10 to 80% of the total enzymatic hydrolysis time.

13. The process according to claim 11 , wherein the other part of the time of the enzymatic hydrolysis is 2 to 80% of the total enzymatic hydrolysis time.

14. The process according to claim 13 , wherein the other part of the time is:
a) 12 to 50% of the total enzymatic hydrolysis time when the oxygen is added in the second half of time of the enzymatic hydrolysis;
b) 2 to 30% of the total enzymatic hydrolysis time when the oxygen is added in the first half of time of the enzymatic hydrolysis; or
c) a combination of a and b.

15. The process according to claim 13 , wherein the other part of the time is:
a) 20 to 40% when the oxygen is added in the second half of time of the enzymatic hydrolysis;
b) 4 to 25% of the total enzymatic hydrolysis time when the oxygen is added in the first half of time of the enzymatic hydrolysis; or
c) a combination of a and b.

16. The process according to claim 13 , wherein the other part of the time is:
a) 20 to 40% when the oxygen is added in the second half of time of the enzymatic hydrolysis;
b) 5 to 20% of the total enzymatic hydrolysis time when the oxygen is added in the first half of time of the enzymatic hydrolysis; or
c) a combination of a and b.

17. The process according to claim 11 , wherein the part of the time wherein less oxygen is added is 20 to 80% of the total enzymatic hydrolysis time.

18. The process according to claim 11 , wherein the part of the time wherein less oxygen is added is 30 to 80% of the total enzymatic hydrolysis time.

19. The process according to claim 11 , wherein the part of the time wherein less oxygen is added is 40 to 80% of the total enzymatic hydrolysis time.

20. The process according to claim 11 , wherein the other part of the time of the enzymatic hydrolysis is 4 to 60% of the total enzymatic hydrolysis time.

21. The process according to claim 11 , wherein the other part of the time of the enzymatic hydrolysis is 8 to 50% of the total enzymatic hydrolysis time.

22. The process according to claim 11 , wherein the other part of the time of the enzymatic hydrolysis is 10 to 50% of the total enzymatic hydrolysis time.

23. The process according to claim 1 , wherein during part of the time of the enzymatic hydrolysis no oxygen is added to the lignocellulosic material compared to the other part of the time of the enzymatic hydrolysis.

24. The process according to claim 23 , wherein the part of the time wherein no oxygen is added is 10 to 80% of the total enzymatic hydrolysis time.

25. The process according to claim 23 , wherein the part of the time wherein no oxygen is added is 20 to 80% of the total enzymatic hydrolysis time.

26. The process according to claim 23 , wherein the part of the time wherein no oxygen is added is 30 to 80% of the total enzymatic hydrolysis time.

27. The process according to claim 23 , wherein the part of the time wherein no oxygen is added is 40 to 80% of the total enzymatic hydrolysis time.

28. The process according to claim 23 , wherein the other part of the time of the enzymatic hydrolysis is 2 to 80% of the total enzymatic hydrolysis time.

29. The process according to claim 23 , wherein the other part of the time of the enzymatic hydrolysis is 4 to 60% of the total enzymatic hydrolysis time.

30. The process according to claim 23 , wherein the other part of the time of the enzymatic hydrolysis is 8 to 50% of the total enzymatic hydrolysis time.

31. The process according to claim 23 , wherein the other part of the time of the enzymatic hydrolysis is 10 to 50% of the total enzymatic hydrolysis time.

32. The process according to claim 1 , wherein oxygen is consumed in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material.

33. The process according to claim 1 , wherein oxygen is consumed in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material.

34. The process according to claim 1 , wherein oxygen is consumed in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material.

35. The process according to

Assignees

• Dsm Ip Assets Bv

Inventors

• Noordam Bertus
• Berkhout Michael Petrus Jozef
• Hofmeester Joseph Johannes Maria

Classifications

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• D21C5/005

  Treatment of cellulose-containing material with microorganisms or enzymes · CPC title

• C12M45/06

  by chemical means or hydrolysis · CPC title