Cell culturing device, culturing vessel, and holding vessel

Patent metadata

Field	Value
Publication number	US-10087410-B2
Application number	US-201314770627-A
Country	US
Kind code	B2
Filing date	Mar 25, 2013
Priority date	Mar 25, 2013
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610 , and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.

First claim

The invention claimed is:

1. A cell culture apparatus, comprising:
a culture vessel base which is arranged in a culture space and on which a plurality of culture vessels are placed; and
a plurality of heat storage portions for warming the plurality of culture vessels, respectively, each heat storage portion including a heat storage material and a receiving portion to be in contact with a culture vessel placed thereon to conduct heat from the heat storage material to the culture vessel placed thereon;
wherein the plurality of heat storage portions, which are placed on the culture vessel base in the culture space during culture and contains the respective culture vessels, are provided so that at least one storage portion is removable from the culture space.

2. The cell culture apparatus according to claim 1 , wherein
the culture vessel base includes a guide portion for allowing the plurality of heat storage portions to be arranged in a predetermined region of the culture space so that culture can be performed in a state in which the culture vessels are contained.

3. The cell culture apparatus according to claim 1 , further comprising:
an observation portion for observing the plurality of culture vessels,
wherein the culture vessel base has a U-shape and is provided so that the plurality of placed culture vessels surround the observation portion in a state in which the culture vessel base is arranged in a predetermined region of the culture space.

4. The cell culture apparatus according to claim 3 ,
wherein each culture vessel is eccentrically provided in the heat storage portion to be positioned on the observation portion side of the cell culture apparatus.

5. The cell culture apparatus according to claim 3 ,
wherein the observation portion is movable relative to the plurality of culture vessels for observing the plurality of culture vessels.

6. The cell culture apparatus according to claim 5 ,
wherein the observation portion includes a microscope for observing the plurality of culture vessels.

7. The cell culture apparatus according to claim 1 , further comprising
a microscope for observing the plurality of culture vessels,
wherein, in the case where observation is performed with the use of the microscope in the cell culture apparatus, the heat storage portions in a direction in which the microscope approaches are thinner than the heat storage portions in directions other than the direction.

8. The cell culture apparatus according to claim 1 ,
wherein, in the case where at least one of the plurality of heat storage portions which are placed on the culture vessel base during culture and contain the respective culture vessels is taken out from the culture space, a temperature of the culture vessels which are not taken out from the culture vessel base is maintainable.

9. The cell culture apparatus according to claim 1 , wherein
the culture vessels other than the culture vessel to be taken out and the heat storage portions containing the respective culture vessels are integrally provided and are integrally removable from the culture space.

10. The cell culture apparatus according to claim 1 , further comprising:
a heat insulation portion for, in the case where at least one of the plurality of heat storage portions containing the respective culture vessels is taken out from the culture space, covering an outer circumference of the heat storage portion to be taken out.

11. The cell culture apparatus according to claim 10 ,
wherein the heat storage material is provided between each of the plurality of culture vessels provided with the heat storage material and the corresponding heat insulation portion.

12. The cell culture apparatus according to claim 10 ,
wherein the heat insulation portion includes an upper-surface heat insulation portion, a lower-surface heat insulation portion, and a side-surface heat insulation portion
wherein the upper-surface heat insulation portion covers an upper part of the side-surface heat insulation portion, and the lower-surface heat insulation portion cover a lower part of the side-surface heat insulation portion.

13. The cell culture apparatus according to claim 10 ,
wherein a part of the heat insulation portion is removable; and
wherein the heat storage portion which is exposed by removing the part of the heat insulation portion can be heated.

14. The cell culture apparatus according to claim 13 ,
wherein the culture vessel which is exposed by removing the part of the heat insulation portion includes an optically transparent window through which a biological sample in the culture vessel is observable.

15. The cell culture apparatus according to claim 1 ,
wherein each heat storage portion surrounds the culture vessel which is placed on the receiving portion thereof.

16. The cell culture apparatus according to claim 1 ,
wherein each heat storage portion includes a heat storage portion vessel which contains the heat storage material thereof.

17. The cell culture apparatus according to claim 16 ,
wherein the heat storage portion vessel is made of polycarbonate and the receiving portion is made of metal.

18. The cell culture apparatus according to claim 1 ,
wherein each heat storage portion has a trapezoidal shape and the plurality of heat storage portions are arranged in a circular shape.

19. The cell culture apparatus according to claim 1 ,
wherein each heat storage portion has a pair of side surfaces, and the culture vessel contained in said each heat storage portion is eccentrically provided on one side of the pair of side surfaces.

20. The cell culture apparatus according to claim 19 ,
wherein each heat storage portion has a trapezoidal shape and the plurality of heat storage portions are arranged in a circular shape; and
wherein the culture vessel is eccentric on the side surface which is a short side of the trapezoidal shape.

Assignees

• Hitachi Ltd

Inventors

• Nozaki Takayuki
• Zhou Guangbin
• Kiyama Masaharu
• Nakamura Taku
• Takeda Shizu
• Nakajima Ryota
• Sugaya Masakazu
• Terada Koichi

Classifications

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• C12M41/14 Primary

  Incubators; Climatic chambers (per se B01L1/00) · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M23/48

  Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00) · CPC title

• C12M23/58

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title