Nucleic acids encoding IL-22 Fc fusion proteins

Patent metadata

Field	Value
Publication number	US-10087227-B2
Application number	US-201615369236-A
Country	US
Kind code	B2
Filing date	Dec 5, 2016
Priority date	Mar 15, 2013
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

The invention relates to IL-22 polypeptides, IL-22 Fc fusion proteins and IL-22 agonists, composition comprising the same, methods of making and methods of using the composition for the treatment of diseases. The invention also relates to IL-22 receptor associated reagents and methods of use thereof.

First claim

What is claimed is:

1. An isolated nucleic acid encoding an interleukin (IL)-22 Fc fusion protein the IL-22 Fc fusion protein comprising an IL-22 polypeptide linked by a linker to an IgG4 Fc region that is not glycosylated, wherein the IL-22 Fc fusion protein binds to IL-22 receptor and comprises an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:8, and wherein the linker consists of the amino acid sequence of RVESKYGPP (SEQ ID NO:44).

2. The nucleic acid of claim 1 , wherein the amino acid sequence has at least 99% sequence identity to the amino acid sequence of SEQ ID NO:8.

3. The nucleic acid of claim 1 , wherein the amino acid residue at position 297 as in the EU index of the IgG4 Fc region is Gly.

4. The nucleic acid of claim 1 , wherein the amino acid residue at position 297 as in the EU index of the IgG4 Fc region is Ala.

5. The nucleic acid of claim 1 , wherein the IL-22 polypeptide is a human IL-22 polypeptide.

6. The nucleic acid of claim 1 , wherein the IL-22 polypeptide comprises the amino acid sequence of SEQ ID NO:4.

7. The nucleic acid of claim 1 , wherein the IL-22 receptor is a human IL-22 receptor.

8. A vector comprising the nucleic acid of claim 1 .

9. An isolated host cell comprising the vector of claim 8 .

10. The host cell of claim 9 , wherein the host cell is a prokaryotic cell or eukaryotic cell.

11. The host cell of claim 10 , wherein the host cell is an E. coli cell or a Chinese hamster ovary (CHO) cell.

12. The host cell of claim 11 , wherein the host cell is a CHO cell.

13. A method of making an IL-22 Fc fusion protein comprising the step of culturing the host cell of claim 9 under conditions suitable for expression of the IL-22 Fc fusion protein encoded by the nucleic acid.

14. The method of claim 13 , further comprising the step of obtaining the IL-22 Fc fusion protein from the cell culture or culture medium.

15. The method of claim 13 , wherein the host cell is an E. coli cell.

16. The method of claim 13 , wherein the host cell is a CHO cell.

17. An isolated nucleic acid encoding an IL-22 Fc fusion protein that comprises the amino acid sequence of SEQ ID NO:8.

18. An isolated nucleic acid encoding an IL-22 Fc fusion protein consisting of the amino acid sequence of SEQ ID NO:8.

19. An isolated nucleic acid comprising the polynucleotide sequence of SEQ ID NO:7.

20. An isolated nucleic acid encoding an IL-22 Fc fusion protein that comprises the amino acid sequence of SEQ ID NO:10.

21. The nucleic acid of claim 20 , comprising the polynucleotide sequence of SEQ ID NO:9.

22. An isolated nucleic acid encoding an IL-22 Fc fusion protein consisting of the amino acid sequence of SEQ ID NO:10.

23. An isolated nucleic acid encoding an IL-22 Fc fusion protein that comprises the amino acid sequence of SEQ ID NO:16.

24. An isolated nucleic acid encoding an IL-22 Fc fusion protein consisting of the amino acid sequence of SEQ ID NO:16.

25. An isolated nucleic acid comprising the polynucleotide sequence of SEQ ID NO:15.

26. An isolated nucleic acid encoding an IL-22 Fc fusion protein that comprises an IL-22 polypeptide linked to an IgG4 Fc region by a linker, wherein the Fc region is not glycosylated, and wherein the linker consists of the amino acid sequence RVESKYGPP (SEQ ID NO: 44).

Assignees

• Genentech Inc

Inventors

• Scheer Justin
• Ouyang Wenjun
• Vandlen Richard
• Hass Philip E
• Stefanich Eric Gary
• Wang Xiaoting

Classifications

• A61P3/06

  Antihyperlipidemics · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P9/12

  Antihypertensives · CPC title