Separation device and separation method

Patent metadata

Field	Value
Publication number	US-10086313-B2
Application number	US-201414897844-A
Country	US
Kind code	B2
Filing date	Jun 19, 2014
Priority date	Jul 5, 2013
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

An object of the present invention is to separate a material to be separated at a low cost and constant accuracy when the material to be separated is separated from a mobile phase containing the material to be separated through the passing of the mobile phase through a stationary phase, even if the mobile phase has a large volume. A separation device characterized in that a separation column provided with a stationary phase having a volume capable of processing the entire volume of a mobile phase containing a material to be separated is provided, the separation column is replaceable, and the usage count of the stationary phase reaches a lifetime count through the processing of one batch.

First claim

The invention claimed is:

1. A method of separation with a separation device comprising:
determining a number of plural columns and a total column volume to be provided in the separation device is provided in an amount satisfying the following equations:
N×T 2≥( T 1+ T 2+ T 3+ T 4+ T 5), and

Ct·Vb≤Am·Vc·R·N,

where N is a number of plural columns, T1 is an equilibrium time of the separation column, T2 is an adsorption time of the material to be separated, T3 is a washing time, T4 is an elution time of the material to be separated, T5 is a regeneration time of the separation column, Ct is a concentration of the material to be separated in the mobile phase, Vb is a volume of the mobile phase per one batch, Am is a dynamic adsorption amount of the material to be separated per unit volume of the separation column, Vc is a volume of the stationary phase per one separation column, and R is a number of reuses of one separation column, one column volume being determined as 1/R;
providing a separation device composed of N plural columns provided with a stationary phase having a total volume sized (Vc·N) to process an entire volume of a selected mobile phase Vb containing a selected material to be separated.

2. The method of claim 1 , further comprising:
providing a plurality of pipes respectively connected to each of the plural columns;
providing a plurality of switching valves respectively provided on the plurality of pipes; and
providing a computer for controlling communication between the pipes by the plurality of switching valves.

Assignees

• Hitachi Ltd

Inventors

• Shibuya Keisuke
• Tada Yasuhiko
• Kondo Takeyuki
• Murakami Sei

Classifications

• C07K1/22

  Affinity chromatography or related techniques based upon selective absorption processes · CPC title

• B01D15/1885

  placed in parallel · CPC title

• G01N2030/8813

  biological materials · CPC title

• B01D15/20

  relating to the conditioning of the sorbent material · CPC title

• B01D15/1821

  Simulated moving beds · CPC title